################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BciVI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsgI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 718 GTCTAC GT^[AC][GT]AC AflIII 773 ACGTGT A^C[GA][CT]GT AlwNI 427 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 799 GAGCTC G[AT]GC[AT]^C BbeI 25 GGCGCC GGCGC^C BbvCI 419 CCTCAGC CCTCAGC(-5/-2) BcgI 395 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 308 TGATCA T^GATCA BlpI 468 GCTGAGC GC^T[ACGT]AGC Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC BseRI 586 GAGGAG GAGGAG(10/8) BsiHKAI 799 GAGCTC G[AT]GC[AT]^C BsiWI 38 CGTACG C^GTACG BsmAI 121 GAGAC GTCTC(1/5) BsmBI 121 GAGACG CGTCTC(1/5) BsrFI 561 GCCGGC [GA]^CCGG[CT] BsrI 830 CCAGT ACTGG(1/-1) CelII 468 GCTGAGC GC^T[ACGT]AGC Cfr10I 561 GCCGGC [GA]^CCGG[CT] DraIII 514 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 203 CGGCCG C^GGCCG EclXI 203 CGGCCG C^GGCCG Eco52I 203 CGGCCG C^GGCCG FbaI 308 TGATCA T^GATCA HphI 311 TCACC GGTGA(8/7) KasI 25 GGCGCC G^GCGCC MslI 312 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 136 CAGCTG C[AC]G^C[GT]G NaeI 561 GCCGGC GCC^GGC NarI 25 GGCGCC GG^CGCC NcoI 743 CCATGG C^CATGG NgoMIV 561 GCCGGC G^CCGGC NspI 473 GCATGC [GA]CATG^[CT] PaeR7I 583 CTCGAG C^TCGAG PpuMI 488 GGGACCC [GA]G^G[AT]CC[CT] PstI 757 CTGCAG CTGCA^G PvuI 852 CGATCG CGAT^CG PvuII 136 CAGCTG CAG^CTG SacI 799 GAGCTC GAGCT^C SexAI 50 ACCTGGT A^CC[AT]GGT SfcI 757 CTGCAG C^T[GA][CT]AG SfoI 25 GGCGCC GGC^GCC SmaI 664 CCCGGG CCC^GGG SmlI 583 CTCGAG C^T[CT][GA]AG SphI 473 GCATGC GCATG^C TaqI 584 TCGA T^CGA TthHB8I 584 TCGA T^CGA XcmI 638 CCACCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 583 CTCGAG C^TCGAG XmaCI 664 CCCGGG C^CCGGG XmaI 664 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 25 GGCGCC G[GA]^CG[CT]C 178 GACGCC ApaI 692 GGGCCC GGGCC^C 870 GGGCCC AvaI 583 CTCGAG C^[CT]CG[GA]G 664 CCCGGG BalI 678 TGGCCA TGG^CCA 740 TGGCCA BanI 25 GGCGCC G^G[CT][GA]CC 558 GGTGCC BbrPI 451 CACGTG CAC^GTG 772 CACGTG BsaHI 25 GGCGCC G[GA]^CG[CT]C 178 GACGCC BsiEI 203 CGGCCG CG[GA][CT]^CG 852 CGATCG BsmFI 272 GGGAC GGGAC(10/14) 488 GGGAC BsoBI 583 CTCGAG C^[CT]CG[GA]G 664 CCCGGG BssHII 11 GCGCGC G^CGCGC 62 GCGCGC BstXI 641 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 743 CCATGGTGCTGG DdeI 420 CTCAG C^T[ACGT]AG 469 CTGAG EarI 289 CTCTTC CTCTTC(1/4) 724 CTCTTC EcoNI 582 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 696 CCTCTACAAGG EcoT14I 29 CCTTGG C^C[AT][AT]GG 743 CCATGG HaeII 25 GGCGCC [GA]GCGC^[CT] 428 AGCGCC HgaI 123 GACGC GACGC(5/10) 178 GACGC Hin1I 25 GGCGCC G[GA]^CG[CT]C 178 GACGCC HinfI 447 GACTC G^A[ACGT]TC 883 GAGTC Ksp632I 289 CTCTTC CTCTTC(1/4) 724 CTCTTC MluNI 678 TGGCCA TGG^CCA 740 TGGCCA MscI 678 TGGCCA TGG^CCA 740 TGGCCA PleI 447 GACTC GAGTC(4/5) 883 GAGTC PmaCI 451 CACGTG CAC^GTG 772 CACGTG PmlI 451 CACGTG CAC^GTG 772 CACGTG SfaNI 349 GATGC GCATC(5/9) 607 GATGC StuI 359 AGGCCT AGG^CCT 590 AGGCCT StyI 29 CCTTGG C^C[AT][AT]GG 743 CCATGG TspRI 341 CACTG CA[GC]TG[ACGT][ACGT]^ 437 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I