(Circular) (Six-base) MAPSORT of: pFN21AE5512.seq Check: 7988 from: 1 to: 5785 pFN21AE5512 5785 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 3128 1937 Size: 1086 105 4594 Fragments arranged by size: 4594 1086 105 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2325 3848 5232 5500 1632 Size: 591 102 1523 1384 268 1917 Fragments arranged by size: 1917 1523 1384 591 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2026 3528 5100 2026 Size: 1502 1572 2711 Fragments arranged by size: 2711 1572 1502 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2687 3094 3320 3472 5581 1493 Size: 1194 407 226 152 2109 1697 Fragments arranged by size: 2109 1697 1194 407 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2913 3106 4561 618 Size: 325 217 627 1126 193 1455 1842 Fragments arranged by size: 1842 1455 1126 627 325 217 193 BanII G_rGCy'C Cuts at: 1391 2395 3104 1391 Size: 1004 709 4072 Fragments arranged by size: 4072 1004 709 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 928 Size: 971 301 4513 Fragments arranged by size: 4513 971 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BfrBI ATG'CAT Cuts at: 2629 2629 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2053 5780 2053 Size: 3727 2058 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2378 2916 3003 3846 5002 1790 Size: 588 538 87 843 1156 2573 Fragments arranged by size: 2573 1156 843 588 538 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 2666 3516 Size: 416 53 83 399 24 669 580 850 Cuts at: 3516 4474 442 Size: 958 1753 Fragments arranged by size: 1753 958 850 669 580 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2392 2906 4445 1239 Size: 1153 514 1539 2579 Fragments arranged by size: 2579 1539 1153 514 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2034 3910 4775 5073 2034 Size: 1876 865 298 2746 Fragments arranged by size: 2746 1876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3005 3035 1178 Size: 30 165 30 1602 30 3928 Fragments arranged by size: 3928 1602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5785 BseYI C'CCAG_C Cuts at: 2909 4988 5484 2909 Size: 2079 496 3210 Fragments arranged by size: 3210 2079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2940 3018 3846 3931 5002 2940 Size: 78 828 85 1071 3723 Fragments arranged by size: 3723 1071 828 85 78 BsmI GAATG_Cn' Cuts at: 2629 3296 3389 2629 Size: 667 93 5025 Fragments arranged by size: 5025 667 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2378 2395 2916 2940 3003 3018 3104 Size: 399 588 17 521 24 63 15 86 Cuts at: 3104 3846 3931 5002 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 521 399 86 85 63 24 17 15 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3140 844 Size: 2296 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2875 4429 4616 1700 Size: 110 1065 1554 187 2869 Fragments arranged by size: 2869 1554 1065 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2197 2170 Size: 27 5758 BstYI r'GATC_y Cuts at: 1972 2053 3115 3482 3867 3884 5336 5422 5434 Size: 81 1062 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1062 385 367 346 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 3301 4095 4115 3301 Size: 794 20 4971 Fragments arranged by size: 4971 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 2686 3089 2686 Size: 403 5382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750 Size: 54 1425 50 275 18 1402 889 1629 Cuts at: 5750 8 Size: 43 Fragments arranged by size: 1629 1425 1402 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2783 2939 3715 1353 Size: 651 779 156 776 3423 Fragments arranged by size: 3423 779 776 651 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2325 2392 2906 3848 4445 5232 Size: 393 591 102 67 514 942 597 787 Cuts at: 5232 5500 1239 Size: 268 1524 Fragments arranged by size: 1524 942 787 597 591 514 393 268 102 67 EcoO109I rG'GnC_Cy Cuts at: 2023 2023 Size: 5785 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2134 4932 1678 Size: 456 2798 2531 Fragments arranged by size: 2798 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2382 2414 2726 2758 3005 Size: 32 135 32 810 32 312 32 247 Cuts at: 3005 3037 4426 4458 4500 4532 1373 Size: 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1389 810 312 247 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2452 3129 3375 669 Size: 988 281 514 677 246 3079 Fragments arranged by size: 3079 988 677 514 281 246 HindIII A'AGCT_T Cuts at: 748 2672 748 Size: 1924 3861 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 2306 3384 2306 Size: 1078 4707 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2362 4899 5083 1305 Size: 1057 2537 184 2007 Fragments arranged by size: 2537 2007 1057 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3963 Fragments arranged by size: 3963 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3743 4102 4261 518 Size: 648 2577 359 159 2042 Fragments arranged by size: 2577 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2887 3201 3878 5026 5271 1606 Size: 463 818 314 677 1148 245 2120 Fragments arranged by size: 2120 1148 818 677 463 314 245 NaeI GCC'GGC Cuts at: 2877 2877 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NgoMIV G'CCGG_C Cuts at: 2875 2875 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NsiI A_TGCA'T Cuts at: 2631 2631 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 2157 3137 830 Size: 1327 980 3478 Fragments arranged by size: 3478 1327 980 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2069 2069 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SapI GCTCTTCn'nnn_ Cuts at: 2939 2939 Size: 5785 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2153 2800 3133 4268 4949 5140 826 Size: 219 1108 647 333 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 681 647 333 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2049 3889 4790 5052 820 Size: 197 926 106 1840 901 262 1553 Fragments arranged by size: 1840 1553 926 901 262 197 106 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 2987 1517 Size: 1470 4315 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2820 2860 3224 513 Size: 552 939 111 705 40 364 3074 Fragments arranged by size: 3074 939 705 552 364 111 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 2106 4031 Size: 274 80 33 51 1443 117 12 1925 Cuts at: 4031 5743 96 Size: 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 117 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 2555 2587 5745 5777 2212 Size: 32 311 32 3158 32 2220 Fragments arranged by size: 3158 2220 311 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2273 1316 Size: 957 4828 XbaI T'CTAG_A Cuts at: 2518 3121 2518 Size: 603 5182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI