(Circular) (Six-base) MAPSORT of: pFN21AE4334.seq Check: 107 from: 1 to: 5737 pFN21AE4334 5737 bp With 182 enzymes: SgfI * March 28, 2012 13:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3800 5184 5452 1632 Size: 2168 1384 268 1917 Fragments arranged by size: 2168 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 2285 2537 4636 5613 1203 Size: 1082 252 2099 977 1327 Fragments arranged by size: 2099 1327 1082 977 252 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 2836 4466 2836 Size: 1630 4107 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4363 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2460 Size: 32 5705 AlwNI CAG_nnn'CTG Cuts at: 2137 2600 2655 3480 5052 2137 Size: 463 55 825 1572 2822 Fragments arranged by size: 2822 1572 825 463 55 ApaLI G'TGCA_C Cuts at: 2433 2752 3794 4950 2433 Size: 319 1042 1156 3220 Fragments arranged by size: 3220 1156 1042 319 ApoI r'AATT_y Cuts at: 1493 3046 3272 3424 5533 1493 Size: 1553 226 152 2109 1697 Fragments arranged by size: 2109 1697 1553 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 3062 1870 Size: 73 1119 4545 Fragments arranged by size: 4545 1119 73 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2255 3058 4513 618 Size: 325 217 627 232 236 803 1455 1842 Fragments arranged by size: 1842 1455 803 627 325 236 232 217 BanII G_rGCy'C Cuts at: 1391 3056 1391 Size: 1665 4072 BbeI G_GCGC'C Cuts at: 2259 2259 Size: 5737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2973 3000 928 Size: 971 1074 27 3665 Fragments arranged by size: 3665 1074 971 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 1903 Size: 34 1991 34 3678 Fragments arranged by size: 3678 1991 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 2685 4348 Size: 107 122 71 631 605 360 653 1663 Cuts at: 4348 136 Size: 1525 Fragments arranged by size: 1663 1525 653 631 605 360 122 107 71 BglII A'GATC_T Cuts at: 2690 5732 2690 Size: 3042 2695 BlpI GC'TnA_GC Cuts at: 2186 3154 2186 Size: 968 4769 Bme1580I G_kGCm'C Cuts at: 1790 2086 2437 2756 2828 3798 4954 1790 Size: 296 351 319 72 970 1156 2573 Fragments arranged by size: 2573 1156 970 351 319 296 72 BmgBI CAC'GTC Cuts at: 1318 2212 1318 Size: 894 4843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2632 4397 1239 Size: 1393 1765 2579 Fragments arranged by size: 2579 1765 1393 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4400 882 Size: 617 2901 2219 Fragments arranged by size: 2901 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3528 493 Size: 711 2324 2702 Fragments arranged by size: 2702 2324 711 BsaBI GATnn'nnATC Cuts at: 5731 5731 Size: 5737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 3544 3926 4561 Size: 53 83 186 936 723 1288 382 635 Cuts at: 4561 275 Size: 1451 Fragments arranged by size: 1451 1288 936 723 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2942 4170 4568 4842 4989 5400 2942 Size: 1228 398 274 147 411 3279 Fragments arranged by size: 3279 1228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5512 Fragments arranged by size: 5512 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2219 2902 2996 1186 Size: 991 42 683 94 3927 Fragments arranged by size: 3927 991 683 94 42 BseYI C'CCAG_C Cuts at: 2090 2683 4940 5436 2090 Size: 593 2257 496 2391 Fragments arranged by size: 2391 2257 593 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 2182 3187 3948 4976 1540 Size: 275 186 124 57 1005 761 1028 2301 Fragments arranged by size: 2301 1028 1005 761 275 186 124 57 BsiHKAI G_wGCw'C Cuts at: 2192 2437 2756 3798 3883 4954 2192 Size: 245 319 1042 85 1071 2975 Fragments arranged by size: 2975 1071 1042 319 245 85 BsmI GAATG_Cn' Cuts at: 3248 3341 3248 Size: 93 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2192 2437 2756 2828 3056 3798 Size: 399 296 106 245 319 72 228 742 Cuts at: 3798 3883 4954 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 296 245 228 106 85 72 BspEI T'CCGG_A Cuts at: 2942 5400 2942 Size: 2458 3279 BspHI T'CATG_A Cuts at: 3626 5356 3626 Size: 1730 4007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2684 3092 844 Size: 1840 408 3489 Fragments arranged by size: 3489 1840 408 BsrBI CCG'CTC Cuts at: 1394 1823 3624 1394 Size: 429 1801 3507 Fragments arranged by size: 3507 1801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2431 4232 4406 65 Size: 1183 1183 1801 174 1396 Fragments arranged by size: 1801 1396 1183 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2177 2181 2799 4381 4568 1700 Size: 110 367 4 618 1582 187 2869 Fragments arranged by size: 2869 1582 618 367 187 110 4 BsrGI T'GTAC_A Cuts at: 96 2139 96 Size: 2043 3694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 2194 3797 4809 2194 Size: 1603 1012 3122 Fragments arranged by size: 3122 1603 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstXI CCAn_nnnn'nTGG Cuts at: 2030 2542 2756 2030 Size: 512 214 5011 Fragments arranged by size: 5011 512 214 BstYI r'GATC_y Cuts at: 1972 2690 2945 3067 3434 3819 3836 5288 5374 Size: 718 255 122 367 385 17 1452 86 Cuts at: 5374 5386 5732 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 255 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2914 2914 Size: 5737 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4246 Fragments arranged by size: 4246 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4998 Fragments arranged by size: 4998 683 56 BtsI GCAGTG_nn' Cuts at: 2086 3253 4047 4067 2086 Size: 1167 794 20 3756 Fragments arranged by size: 3756 1167 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DraIII CAC_nnn'GTG Cuts at: 2196 2196 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2179 3184 Size: 54 1425 50 275 18 292 57 1005 Cuts at: 3184 4073 5702 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1005 889 292 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2122 2179 3184 1537 Size: 275 310 57 1005 4090 Fragments arranged by size: 4090 1005 310 275 57 EarI CTCTTCn'nnn_ Cuts at: 1353 2234 2973 3667 1353 Size: 881 739 694 3423 Fragments arranged by size: 3423 881 739 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2632 3800 4397 5184 5452 1239 Size: 393 1000 1168 597 787 268 1524 Fragments arranged by size: 1524 1168 1000 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2666 2666 Size: 5737 EcoO109I rG'GnC_Cy Cuts at: 2134 2134 Size: 5737 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 EcoRV GAT'ATC Cuts at: 2746 2746 Size: 5737 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 2259 4884 1678 Size: 581 2625 2531 Fragments arranged by size: 2625 2531 581 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2566 2598 2822 2854 4378 Size: 32 135 32 994 32 224 32 1524 Cuts at: 4378 4410 4452 4484 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 994 224 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5737 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KasI G'GCGC_C Cuts at: 2255 2255 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 2820 4851 5035 1305 Size: 1092 423 2031 184 2007 Fragments arranged by size: 2031 2007 1092 423 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3915 Fragments arranged by size: 3915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 3695 4054 4213 518 Size: 648 1374 1155 359 159 2042 Fragments arranged by size: 2042 1374 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2127 2349 3153 3830 4978 5223 1606 Size: 521 222 804 677 1148 245 2120 Fragments arranged by size: 2120 1148 804 677 521 245 222 NaeI GCC'GGC Cuts at: 2183 2183 Size: 5737 NarI GG'CG_CC Cuts at: 2256 2256 Size: 5737 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4246 Fragments arranged by size: 4246 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NgoMIV G'CCGG_C Cuts at: 2181 2181 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 2541 3095 4640 1132 Size: 97 1312 554 1545 2229 Fragments arranged by size: 2229 1545 1312 554 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5737 PciI A'CATG_T Cuts at: 2537 4636 2537 Size: 2099 3638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2068 1108 Size: 960 4777 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2134 Size: 5737 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 2174 3089 830 Size: 1344 915 3478 Fragments arranged by size: 3478 1344 915 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2170 3004 3085 4220 4901 5092 826 Size: 219 1125 834 81 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 834 681 219 191 81 SfoI GGC'GCC Cuts at: 2257 2257 Size: 5737 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4742 5004 820 Size: 197 926 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 3095 1132 Size: 1963 3774 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 2004 2485 3176 513 Size: 552 939 481 691 3074 Fragments arranged by size: 3074 939 691 552 481 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 2216 3764 3781 3934 4119 4564 Size: 1255 582 54 1548 17 153 185 445 Cuts at: 4564 325 Size: 1498 Fragments arranged by size: 1548 1498 1255 582 445 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 3983 5695 Size: 274 80 33 51 1443 162 1844 1712 Cuts at: 5695 96 Size: 138 Fragments arranged by size: 1844 1712 1443 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5697 5729 5697 Size: 32 5705 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 3028 3073 3028 Size: 45 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2542 2756 2542 Size: 214 5523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 3866 3866 Size: 5737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI