Restriction map for
pFN21AE4351
(Circular) (Six-base) MAPSORT of: pFN21AE4351.seq Check: 5211 from: 1 to: 5800
pFN21AE4351 5800 bp
With 182 enzymes: SgfI *
March 28, 2012 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3022 3022
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 2688 3927 4300 1563
Size: 1125 1239 373 3063
Fragments arranged by size:
3063 1239 1125 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 2412 2478 3863 5247 5515 1632
Size: 468 312 66 1385 1384 268 1917
Fragments arranged by size:
1917 1385 1384 468 312 268 66
AfeI AGC'GCT
Cuts at: 2757 2757
Size: 5800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2674 3543 5115 2674
Size: 869 1572 3359
Fragments arranged by size:
3359 1572 869
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2129 2238 2329 3109 3335 3487 5596 1493
Size: 636 109 91 780 226 152 2109 1697
Fragments arranged by size:
2109 1697 780 636 226 152 109 91
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3121 4576 618
Size: 325 217 627 1334 1455 1842
Fragments arranged by size:
1842 1455 1334 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2663 3119 1391
Size: 1272 456 4072
Fragments arranged by size:
4072 1272 456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 2982 928
Size: 971 255 828 3746
Fragments arranged by size:
3746 971 828 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2665 1548
Size: 1117 4683
BfrBI ATG'CAT
Cuts at: 2312 2312
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 5795 2260
Size: 3535 2265
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3021 3021
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2250 4460 1239
Size: 1011 2210 2579
Fragments arranged by size:
2579 2210 1011
Bpu10I CC'TnA_GC
Cuts at: 3018 3018
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2982 1186
Size: 1796 4004
BseYI C'CCAG_C
Cuts at: 5003 5499 5003
Size: 496 5304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3861 3946 5017 2031
Size: 1830 85 1071 2814
Fragments arranged by size:
2814 1830 1071 85
BsiWI C'GTAC_G
Cuts at: 2864 2864
Size: 5800
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2663 3119 3861 3946 5017 1391
Size: 399 241 632 456 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 632 456 399 241 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3022 3155 844
Size: 2178 133 3489
Fragments arranged by size:
3489 2178 133
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 2639 96
Size: 2543 3257
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2260 2453 3130 3497 3882 3899 5351 5437
Size: 288 193 677 367 385 17 1452 86
Cuts at: 5437 5449 5795 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 677 385 367 346 288 193
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2871 3316 4110 4130 2871
Size: 445 794 20 4541
Fragments arranged by size:
4541 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3247 4136 5765
Size: 54 1425 50 275 18 1417 889 1629
Cuts at: 5765 8
Size: 43
Fragments arranged by size:
1629 1425 1417 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2617 3730 1353
Size: 723 541 1113 3423
Fragments arranged by size:
3423 1113 723 541
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2250 2412 2478 3863 4460 5247
Size: 393 468 150 162 66 1385 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 1385 787 597 468 393 268 162
150 66
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
EcoRV GAT'ATC
Cuts at: 2181 2833 2181
Size: 652 5148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2525 2525
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2012 2759 4947 1678
Size: 334 747 2188 2531
Fragments arranged by size:
2531 2188 747 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2285 2310 2317 4441
Size: 32 135 32 706 7 25 7 2124
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2124 706 135 42 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2952 3026 4914 5098 1305
Size: 1647 74 1888 184 2007
Fragments arranged by size:
2007 1888 1647 184 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2359 3758 4117 4276 518
Size: 648 1193 1399 359 159 2042
Fragments arranged by size:
2042 1399 1193 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2189 2637 3216 3893 5041 5286 1606
Size: 555 28 448 579 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 555 448 245 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NsiI A_TGCA'T
Cuts at: 2314 2314
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2908 1348
Size: 1560 4240
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2565 1108
Size: 1457 4343
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2189 2637 2189
Size: 448 5352
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 2621 4048 5760 2621
Size: 1427 1712 2661
Fragments arranged by size:
2661 1712 1427
SfcI C'TryA_G
Cuts at: 826 1045 3093 3148 4283 4964 5155 826
Size: 219 2048 55 1135 681 191 1471
Fragments arranged by size:
2048 1471 1135 681 219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2004 3239 513
Size: 552 939 1235 3074
Fragments arranged by size:
3074 1235 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3038 3827 3844 3997 4182 4627 325
Size: 1255 1458 789 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2275 2619 2639
Size: 274 80 33 51 1443 298 344 20
Cuts at: 2639 4046 5758 96
Size: 1407 1712 138
Fragments arranged by size:
1712 1443 1407 344 298 274 138 80
51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2156 2188 5760 5792 2156
Size: 32 3572 32 2164
Fragments arranged by size:
3572 2164 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI