(Circular) (Six-base) MAPSORT of: pFN21AB7613.seq Check: 3960 from: 1 to: 5614 pFN21AB7613 5614 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2235 3424 278 Size: 53 83 186 936 699 1189 2468 Fragments arranged by size: 2468 1189 936 699 186 83 53 AccI GT'mk_AC Cuts at: 1937 2292 2957 1937 Size: 355 665 4594 Fragments arranged by size: 4594 665 355 Acc65I G'GTAC_C Cuts at: 2541 2785 2935 2541 Size: 244 150 5220 Fragments arranged by size: 5220 244 150 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2679 3677 5061 5329 1632 Size: 815 232 998 1384 268 1917 Fragments arranged by size: 1917 1384 998 815 268 232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 2592 4513 5490 1203 Size: 1389 1921 977 1327 Fragments arranged by size: 1921 1389 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AlwNI CAG_nnn'CTG Cuts at: 2033 2818 3357 4929 2033 Size: 785 539 1572 2718 Fragments arranged by size: 2718 1572 785 539 ApaI G_GGCC'C Cuts at: 2766 2766 Size: 5614 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2923 3149 3301 5410 1493 Size: 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2721 2939 1870 Size: 73 778 218 4545 Fragments arranged by size: 4545 778 218 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2525 2558 2525 Size: 33 5581 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2323 2541 2785 2935 Size: 325 217 627 261 275 218 244 150 Cuts at: 2935 4390 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 275 261 244 218 217 150 BanII G_rGCy'C Cuts at: 1391 2551 2766 2872 2933 1391 Size: 1160 215 106 61 4072 Fragments arranged by size: 4072 1160 215 106 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2740 3506 4716 2740 Size: 766 1210 3638 Fragments arranged by size: 3638 1210 766 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4225 136 Size: 107 122 71 631 605 2553 1525 Fragments arranged by size: 2553 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2422 5609 2422 Size: 3187 2427 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2766 3675 4831 1790 Size: 976 909 1156 2573 Fragments arranged by size: 2573 1156 976 909 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2765 3345 4303 Size: 416 53 83 399 24 1348 580 958 Cuts at: 4303 442 Size: 1753 Fragments arranged by size: 1753 1348 958 580 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2688 4274 1239 Size: 974 475 1586 2579 Fragments arranged by size: 2579 1586 974 475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 3739 4604 4902 2090 Size: 1649 865 298 2802 Fragments arranged by size: 2802 1649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4277 882 Size: 617 2778 2219 Fragments arranged by size: 2778 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 3421 3803 4438 Size: 53 83 186 936 699 1189 382 635 Cuts at: 4438 275 Size: 1451 Fragments arranged by size: 1451 1189 936 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2908 4047 4445 4719 4866 5277 2908 Size: 1139 398 274 147 411 3245 Fragments arranged by size: 3245 1139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2145 2148 2157 2813 1186 Size: 959 3 9 656 3987 Fragments arranged by size: 3987 959 656 9 3 BseYI C'CCAG_C Cuts at: 4817 5313 4817 Size: 496 5118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 1163 Size: 573 1059 3982 Fragments arranged by size: 3982 1059 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2551 2812 2872 3675 3760 4831 2551 Size: 261 60 803 85 1071 3334 Fragments arranged by size: 3334 1071 803 261 85 60 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2551 2766 2812 2872 2933 3675 3760 Size: 399 761 215 46 60 61 742 85 Cuts at: 3760 4831 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 761 742 399 215 85 61 60 46 BspEI T'CCGG_A Cuts at: 2908 5277 2908 Size: 2369 3245 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2969 844 Size: 2125 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4109 4283 65 Size: 1183 2861 174 1396 Fragments arranged by size: 2861 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4258 4445 1700 Size: 110 2448 187 2869 Fragments arranged by size: 2869 2448 187 110 BsrGI T'GTAC_A Cuts at: 96 2064 96 Size: 1968 3646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 3674 4686 3674 Size: 1012 4602 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5614 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 2269 1184 Size: 1085 4529 BstYI r'GATC_y Cuts at: 1972 2359 2422 2944 3311 3696 3713 5165 5251 Size: 387 63 522 367 385 17 1452 86 Cuts at: 5251 5263 5609 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 522 387 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2303 2303 Size: 5614 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 2740 513 Size: 552 939 519 217 3387 Fragments arranged by size: 3387 939 552 519 217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2319 2354 2568 2603 529 Size: 35 1755 35 214 35 3540 Fragments arranged by size: 3540 1755 214 35 35 35 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DraIII CAC_nnn'GTG Cuts at: 2098 2098 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2487 3061 Size: 54 1425 50 275 18 259 398 574 Cuts at: 3061 3950 5579 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 574 398 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2648 3544 1353 Size: 1295 896 3423 Fragments arranged by size: 3423 1295 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2549 2870 2549 Size: 321 5293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2447 2679 2688 3677 4274 5061 Size: 393 581 234 232 9 989 597 787 Cuts at: 5061 5329 1239 Size: 268 1524 Fragments arranged by size: 1524 989 787 597 581 393 268 234 232 9 EcoO109I rG'GnC_Cy Cuts at: 2299 2762 2763 2299 Size: 463 1 5150 Fragments arranged by size: 5150 463 1 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2659 2659 Size: 5614 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 4761 1678 Size: 3083 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2344 2376 2614 2646 4255 Size: 32 135 32 772 32 238 32 1609 Cuts at: 4255 4287 4329 4361 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1609 772 238 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2545 2789 2939 2545 Size: 244 150 5220 Fragments arranged by size: 5220 244 150 MfeI C'AATT_G Cuts at: 2404 3213 2404 Size: 809 4805 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 2489 10 Size: 54 1425 343 259 398 3135 Fragments arranged by size: 3135 1425 398 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 2444 2895 3572 3931 4090 518 Size: 648 1045 233 451 677 359 159 2042 Fragments arranged by size: 2042 1045 677 648 451 359 233 159 MspA1I CmG'CkG Cuts at: 1606 2815 3030 3707 4855 5100 1606 Size: 1209 215 677 1148 245 2120 Fragments arranged by size: 2120 1209 1148 677 245 215 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4123 Fragments arranged by size: 4123 939 552 NdeI CA'TA_TG Cuts at: 387 2575 387 Size: 2188 3426 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2291 2596 2972 4517 1132 Size: 97 1062 305 376 1545 2229 Fragments arranged by size: 2229 1545 1062 376 305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 2592 4513 2592 Size: 1921 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 2098 1108 Size: 990 4624 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2299 Size: 5614 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PspOMI G'GGCC_C Cuts at: 2762 2762 Size: 5614 PstI C_TGCA'G Cuts at: 830 2568 2877 2966 830 Size: 1738 309 89 3478 Fragments arranged by size: 3478 1738 309 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 PvuII CAG'CTG Cuts at: 2815 2815 Size: 5614 SacI G_AGCT'C Cuts at: 2551 2872 2551 Size: 321 5293 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2497 2564 2873 2962 4097 4778 4969 Size: 219 1452 67 309 89 1135 681 191 Cuts at: 4969 826 Size: 1471 Fragments arranged by size: 1471 1452 1135 681 309 219 191 89 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2721 3718 4619 4881 820 Size: 197 926 162 616 997 901 262 1553 Fragments arranged by size: 1553 997 926 901 616 262 197 162 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 2148 2385 1517 Size: 631 237 4746 Fragments arranged by size: 4746 631 237 StyI C'CwwG_G Cuts at: 513 1065 2004 3053 513 Size: 552 939 1049 3074 Fragments arranged by size: 3074 1049 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 3658 3811 3996 4441 325 Size: 1255 2061 17 153 185 445 1498 Fragments arranged by size: 2061 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2064 3860 5572 Size: 274 80 33 51 1443 87 1796 1712 Cuts at: 5572 96 Size: 138 Fragments arranged by size: 1796 1712 1443 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5574 5606 5574 Size: 32 5582 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2712 2712 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 2721 1943 Size: 778 4836 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 2559 3743 2559 Size: 1184 4430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2233 3422 276 Size: 53 83 186 936 699 1189 2468 Fragments arranged by size: 2468 1189 936 699 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI