Restriction map for
pFN21AB7613
(Linear) (Six-base) MAPSORT of: KIBB7613.seq Check: 5125 from: 1 to: 909
KIBB7613.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 0 230 909
Size: 230 679
AccI GT'mk_AC
Cuts at: 0 287 909
Size: 287 622
Acc65I G'GTAC_C
Cuts at: 0 536 780 909
Size: 536 244 129
Fragments arranged by size:
536 244 129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 674 909
Size: 442 232 235
Fragments arranged by size:
442 235 232
AflIII A'CryG_T
Cuts at: 0 587 909
Size: 587 322
AlwNI CAG_nnn'CTG
Cuts at: 0 28 813 909
Size: 28 785 96
Fragments arranged by size:
785 96 28
ApaI G_GGCC'C
Cuts at: 0 761 909
Size: 761 148
AvaI C'yCGr_G
Cuts at: 0 716 909
Size: 716 193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 520 553 909
Size: 520 33 356
Fragments arranged by size:
520 356 33
BanI G'GyrC_C
Cuts at: 0 43 318 536 780 909
Size: 43 275 218 244 129
Fragments arranged by size:
275 244 218 129 43
BanII G_rGCy'C
Cuts at: 0 546 761 867 909
Size: 546 215 106 42
Fragments arranged by size:
546 215 106 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 735 909
Size: 735 174
BglII A'GATC_T
Cuts at: 0 417 909
Size: 417 492
Bme1580I G_kGCm'C
Cuts at: 0 761 909
Size: 761 148
BmrI ACTGGGnnnn_n'
Cuts at: 0 760 909
Size: 760 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 683 909
Size: 208 475 226
Fragments arranged by size:
475 226 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 909
Size: 85 824
BsaHI Gr'CG_yC
Cuts at: 0 227 909
Size: 227 682
BsaWI w'CCGG_w
Cuts at: 0 903 909
Size: 903 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 140 143 152 808 909
Size: 140 3 9 656 101
Fragments arranged by size:
656 140 101 9 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 909
Size: 790 119
BsiHKAI G_wGCw'C
Cuts at: 0 546 807 867 909
Size: 546 261 60 42
Fragments arranged by size:
546 261 60 42
Bsp1286I G_dGCh'C
Cuts at: 0 546 761 807 867 909
Size: 546 215 46 60 42
Fragments arranged by size:
546 215 60 46 42
BspEI T'CCGG_A
Cuts at: 0 903 909
Size: 903 6
BsrGI T'GTAC_A
Cuts at: 0 59 909
Size: 59 850
BstEII G'GTnAC_C
Cuts at: 0 264 909
Size: 264 645
BstYI r'GATC_y
Cuts at: 0 354 417 909
Size: 354 63 492
Fragments arranged by size:
492 354 63
Bsu36I CC'TnA_GG
Cuts at: 0 298 909
Size: 298 611
BtgI C'CryG_G
Cuts at: 0 518 735 909
Size: 518 217 174
Fragments arranged by size:
518 217 174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 314 349 563 598 909
Size: 314 35 214 35 311
Fragments arranged by size:
314 311 214 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 93 909
Size: 93 816
EaeI y'GGCC_r
Cuts at: 0 84 482 909
Size: 84 398 427
Fragments arranged by size:
427 398 84
EarI CTCTTCn'nnn_
Cuts at: 0 643 909
Size: 643 266
EcoICRI GAG'CTC
Cuts at: 0 544 865 909
Size: 544 321 44
Fragments arranged by size:
544 321 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 442 674 683 909
Size: 208 234 232 9 226
Fragments arranged by size:
234 232 226 208 9
EcoO109I rG'GnC_Cy
Cuts at: 0 294 757 758 909
Size: 294 463 1 151
Fragments arranged by size:
463 294 151 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 654 909
Size: 654 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 609 641 909
Size: 339 32 238 32 268
Fragments arranged by size:
339 268 238 32 32
KpnI G_GTAC'C
Cuts at: 0 540 784 909
Size: 540 244 125
Fragments arranged by size:
540 244 125
MfeI C'AATT_G
Cuts at: 0 399 909
Size: 399 510
MscI TGG'CCA
Cuts at: 0 86 484 909
Size: 86 398 425
Fragments arranged by size:
425 398 86
MslI CAynn'nnrTG
Cuts at: 0 206 439 890 909
Size: 206 233 451 19
Fragments arranged by size:
451 233 206 19
MspA1I CmG'CkG
Cuts at: 0 810 909
Size: 810 99
NdeI CA'TA_TG
Cuts at: 0 570 909
Size: 570 339
NspI r_CATG'y
Cuts at: 0 286 591 909
Size: 286 305 318
Fragments arranged by size:
318 305 286
PciI A'CATG_T
Cuts at: 0 587 909
Size: 587 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 909
Size: 93 816
PpuMI rG'GwC_Cy
Cuts at: 0 294 909
Size: 294 615
PspOMI G'GGCC_C
Cuts at: 0 757 909
Size: 757 152
PstI C_TGCA'G
Cuts at: 0 563 872 909
Size: 563 309 37
Fragments arranged by size:
563 309 37
PvuII CAG'CTG
Cuts at: 0 810 909
Size: 810 99
SacI G_AGCT'C
Cuts at: 0 546 867 909
Size: 546 321 42
Fragments arranged by size:
546 321 42
SfcI C'TryA_G
Cuts at: 0 492 559 868 909
Size: 492 67 309 41
Fragments arranged by size:
492 309 67 41
SmlI C'TyrA_G
Cuts at: 0 100 716 909
Size: 100 616 193
Fragments arranged by size:
616 193 100
StuI AGG'CCT
Cuts at: 0 143 380 909
Size: 143 237 529
Fragments arranged by size:
529 237 143
TatI w'GTAC_w
Cuts at: 0 59 909
Size: 59 850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 707 909
Size: 707 202
XhoI C'TCGA_G
Cuts at: 0 716 909
Size: 716 193
XmnI GAAnn'nnTTC
Cuts at: 0 554 909
Size: 554 355
ZraI GAC'GTC
Cuts at: 0 228 909
Size: 228 681
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlwNI ApaI AvaI
BaeI BanI BanII BciVI BglII Bme1580I BmrI BpmI
BpuEI BsaHI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspEI
BsrGI BstEII BstYI Bsu36I BtgI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I KpnI MfeI
MscI MslI MspA1I NdeI NspI PciI PflMI PpuMI
PspOMI PstI PvuII SacI SfcI SmlI StuI TatI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgZI BtsI ClaI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MluI MmeI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TstI Tth111I XbaI XmaI