################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI MaeI MamI MfeI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 1156 GACGTC GACGT^C Acc65I 446 GGTACC G^GTACC AflIII 455 ACGCGT A^C[GA][CT]GT AlwI 1164 GGATC GGATC(4/5) Asp718I 446 GGTACC G^GTACC AspHI 751 GAGCTC G[AT]GC[AT]^C AvaI 523 CCCGAG C^[CT]CG[GA]G BanI 446 GGTACC G^G[CT][GA]CC BbrPI 352 CACGTG CAC^GTG BbsI 46 GAAGAC GAAGAC(2/6) BbvCI 57 GCTGAGG CCTCAGC(-5/-2) BlpI 622 GCTGAGC GC^T[ACGT]AGC Bpu1102I 622 GCTGAGC GC^T[ACGT]AGC BpuAI 46 GAAGAC GAAGAC(2/6) BsaAI 352 CACGTG [CT]AC^GT[GA] BseRI 315 CTCCTC GAGGAG(10/8) BsiEI 600 CGACCG CG[GA][CT]^CG BsiHKAI 751 GAGCTC G[AT]GC[AT]^C BsmFI 1012 GTCCC GGGAC(10/14) BsoBI 523 CCCGAG C^[CT]CG[GA]G BspMI 289 ACCTGC ACCTGC(4/8) BsrBI 568 GAGCGG CCGCTC(-3/-3) BssHII 618 GCGCGC G^CGCGC BssSI 193 CACGAG CACGAG(-5/-1) BstEII 846 GGTGACC G^GT[ACGT]ACC BstPI 846 GGTGACC G^GT[ACGT]ACC BstXI 239 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1164 GGATCT [GA]^GATC[CT] CelII 622 GCTGAGC GC^T[ACGT]AGC DpnII 1165 GATC ^GATC DraIII 841 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 922 CCGCGG C^C[GA][CT]GG EaeI 401 TGGCCG [CT]^GGCC[GA] EcoNI 219 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 846 GGTGACC G^GT[ACGT]ACC HincII 253 GTCAAC GT[CT]^[GA]AC HindII 253 GTCAAC GT[CT]^[GA]AC KpnI 446 GGTACC GGTAC^C KspI 922 CCGCGG CCGC^GG MboI 1165 GATC ^GATC MflI 1164 GGATCT [GA]^GATC[CT] MluI 455 ACGCGT A^CGCGT NaeI 740 GCCGGC GCC^GGC NdeII 1165 GATC ^GATC NgoMIV 740 GCCGGC G^CCGGC NlaIII 990 CATG CATG^ NruI 1174 TCGCGA TCG^CGA PmaCI 352 CACGTG CAC^GTG PmlI 352 CACGTG CAC^GTG PpuMI 983 AGGACCC [GA]G^G[AT]CC[CT] PstI 970 CTGCAG CTGCA^G SacI 751 GAGCTC GAGCT^C SacII 922 CCGCGG CCGC^GG SapI 588 GCTCTTC GCTCTTC(1/4) Sau3AI 1165 GATC ^GATC ScaI 197 AGTACT AGT^ACT StuI 61 AGGCCT AGG^CCT TfiI 996 GATTC G^A[AT]TC Tsp509I 171 AATT ^AATT XcmI 450 CCAGGACGCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1164 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AocI 1015 CCTGAGG CC^T[ACGT]AGG 1054 CCTGAGG BsaI 953 GAGACC GGTCTC(1/5) 1122 GGTCTC BsrFI 740 GCCGGC [GA]^CCGG[CT] 942 GCCGGT Bsu36I 1015 CCTGAGG CC^T[ACGT]AGG 1054 CCTGAGG Cfr10I 740 GCCGGC [GA]^CCGG[CT] 942 GCCGGT DrdI 496 GACGCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1063 GACCTGGGTGTC EarI 326 CTCTTC CTCTTC(1/4) 589 CTCTTC Eco81I 1015 CCTGAGG CC^T[ACGT]AGG 1054 CCTGAGG EcoT14I 68 CCTTGG C^C[AT][AT]GG 1217 CCAAGG HaeII 813 GGCGCT [GA]GCGC^[CT] 915 GGCGCT Ksp632I 326 CTCTTC CTCTTC(1/4) 589 CTCTTC MslI 672 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1111 CACACCAGTG SexAI 50 ACCAGGT A^CC[AT]GGT 842 ACCTGGT StyI 68 CCTTGG C^C[AT][AT]GG 1217 CCAAGG TaqI 782 TCGA T^CGA 1002 TCGA TthHB8I 782 TCGA T^CGA 1002 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI PvuII RsaI Sau96I ScrFI SfcI TseI Tsp45I TspRI