(Circular) (Six-base) MAPSORT of: pFN21AB7768.seq Check: 3126 from: 1 to: 5935 pFN21AB7768 5935 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2303 2303 Size: 5935 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3165 3745 278 Size: 53 83 186 936 1629 580 2468 Fragments arranged by size: 2468 1629 936 580 186 83 53 AccI GT'mk_AC Cuts at: 1937 3278 1937 Size: 1341 4594 Acc65I G'GTAC_C Cuts at: 2451 3256 2451 Size: 805 5130 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2070 2124 2295 2318 3998 5382 5650 1632 Size: 438 54 171 23 1680 1384 268 1917 Fragments arranged by size: 1917 1680 1384 438 268 171 54 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 2460 4834 5811 1203 Size: 1257 2374 977 1327 Fragments arranged by size: 2374 1327 1257 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 3120 1166 Size: 1954 3981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2525 2557 2525 Size: 32 5903 AlwNI CAG_nnn'CTG Cuts at: 2062 2152 2523 3678 5250 2062 Size: 90 371 1155 1572 2747 Fragments arranged by size: 2747 1572 1155 371 90 ApaI G_GGCC'C Cuts at: 2029 2124 3034 2029 Size: 95 910 4930 Fragments arranged by size: 4930 910 95 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 3244 3470 3622 5731 1493 Size: 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2528 3260 1870 Size: 73 585 732 4545 Fragments arranged by size: 4545 732 585 73 BamHI G'GATC_C Cuts at: 3265 3632 3265 Size: 367 5568 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2451 3256 4711 618 Size: 325 217 627 664 805 1455 1842 Fragments arranged by size: 1842 1455 805 664 627 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2124 2634 2760 3034 3254 1391 Size: 638 95 510 126 274 220 4072 Fragments arranged by size: 4072 638 510 274 220 126 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 928 Size: 971 159 4805 Fragments arranged by size: 4805 971 159 BbvCI CC'TCA_GC Cuts at: 2063 2063 Size: 5935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4126 4160 1903 Size: 34 50 34 2105 34 3678 Fragments arranged by size: 3678 2105 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 136 Size: 107 122 71 631 605 2874 1525 Fragments arranged by size: 2874 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 2628 3352 2628 Size: 724 5211 Bme1580I G_kGCm'C Cuts at: 1790 2029 2124 3034 3196 3996 5152 1790 Size: 239 95 910 162 800 1156 2573 Fragments arranged by size: 2573 1156 910 800 239 162 95 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3666 4624 442 Size: 416 53 83 399 24 2249 958 1753 Fragments arranged by size: 2249 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2591 2774 2887 4595 1239 Size: 1352 183 113 1708 2579 Fragments arranged by size: 2579 1708 1352 183 113 Bpu10I CC'TnA_GC Cuts at: 2063 2657 2702 2063 Size: 594 45 5296 Fragments arranged by size: 5296 594 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4060 4925 5223 4060 Size: 865 298 4772 Fragments arranged by size: 4772 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2952 3133 4598 882 Size: 617 1453 181 1465 2219 Fragments arranged by size: 2219 1465 1453 617 181 BsaAI yAC'GTr Cuts at: 493 1204 2359 3726 493 Size: 711 1155 1367 2702 Fragments arranged by size: 2702 1367 1155 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2502 2578 2671 2694 Size: 53 83 186 936 969 76 93 23 Cuts at: 2694 3162 3742 4124 4759 275 Size: 468 580 382 635 1451 Fragments arranged by size: 1451 969 936 635 580 468 382 186 93 83 76 53 23 BsaWI w'CCGG_w Cuts at: 4368 4766 5040 5187 5598 4368 Size: 398 274 147 411 4705 Fragments arranged by size: 4705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 2189 2219 2726 Size: 30 165 30 621 30 135 30 507 Cuts at: 2726 2756 1178 Size: 30 4357 Fragments arranged by size: 4357 621 507 165 135 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2311 1186 Size: 1125 4810 BseYI C'CCAG_C Cuts at: 3133 5138 5634 3133 Size: 2005 496 3434 Fragments arranged by size: 3434 2005 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2083 2737 2933 1163 Size: 573 347 654 196 4165 Fragments arranged by size: 4165 654 573 347 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2608 3385 4146 5174 1540 Size: 275 186 607 777 761 1028 2301 Fragments arranged by size: 2301 1028 777 761 607 275 186 BsiHKAI G_wGCw'C Cuts at: 2760 3996 4081 5152 2760 Size: 1236 85 1071 3543 Fragments arranged by size: 3543 1236 1071 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 2438 2515 3169 2438 Size: 77 654 5204 Fragments arranged by size: 5204 654 77 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2124 2634 2760 3034 3196 3254 Size: 399 239 95 510 126 274 162 58 Cuts at: 3254 3996 4081 5152 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 399 274 239 162 126 95 85 58 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2303 3290 844 Size: 1459 987 3489 Fragments arranged by size: 3489 1459 987 BsrBI CCG'CTC Cuts at: 1394 1823 2575 3822 1394 Size: 429 752 1247 3507 Fragments arranged by size: 3507 1247 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2745 2947 4579 4766 1700 Size: 110 935 202 1632 187 2869 Fragments arranged by size: 2869 1632 935 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 2623 1925 Size: 698 5237 BssSI C'ACGA_G Cuts at: 2198 3995 5007 2198 Size: 1797 1012 3126 Fragments arranged by size: 3126 1797 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5935 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 2851 1184 Size: 1667 4268 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 5935 BstYI r'GATC_y Cuts at: 1972 3169 3265 3632 4017 4034 5486 5572 5584 Size: 1197 96 367 385 17 1452 86 12 Cuts at: 5584 5930 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3021 3060 3021 Size: 39 5896 BtgI C'CryG_G Cuts at: 513 1065 2927 513 Size: 552 1862 3521 Fragments arranged by size: 3521 1862 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 2125 3451 4245 4265 2125 Size: 1326 794 20 3795 Fragments arranged by size: 3795 1326 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2340 2375 529 Size: 35 1776 35 4089 Fragments arranged by size: 4089 1776 35 35 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DraIII CAC_nnn'GTG Cuts at: 2851 2851 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2507 3074 4942 809 Size: 507 1191 567 1868 1802 Fragments arranged by size: 1868 1802 1191 567 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2406 3382 4271 Size: 54 1425 50 275 18 576 976 889 Cuts at: 4271 5900 8 Size: 1629 43 Fragments arranged by size: 1629 1425 976 889 576 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2600 3865 1353 Size: 984 263 1265 3423 Fragments arranged by size: 3423 1265 984 263 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2626 2813 4522 4896 5042 387 Size: 1064 138 1037 187 1709 374 146 1280 Fragments arranged by size: 1709 1280 1064 1037 374 187 146 138 EcoICRI GAG'CTC Cuts at: 2758 2758 Size: 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2124 2295 2318 2591 2774 2887 Size: 393 438 54 171 23 273 183 113 Cuts at: 2887 3998 4595 5382 5650 1239 Size: 1111 597 787 268 1524 Fragments arranged by size: 1524 1111 787 597 438 393 273 268 183 171 113 54 23 EcoNI CCTnn'n_nnAGG Cuts at: 2228 2228 Size: 5935 EcoO109I rG'GnC_Cy Cuts at: 2025 2026 2121 2286 2737 2989 3031 3083 2025 Size: 1 95 165 451 252 42 52 4877 Fragments arranged by size: 4877 451 252 165 95 52 42 1 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2180 2180 Size: 5935 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 2822 2924 5082 1678 Size: 1144 102 2158 2531 Fragments arranged by size: 2531 2158 1144 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2661 2693 3000 Size: 32 135 32 452 32 605 32 307 Cuts at: 3000 3032 3161 3193 4576 4608 4650 4682 1373 Size: 32 129 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 605 452 307 135 129 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 3279 3525 669 Size: 988 281 322 1019 246 3079 Fragments arranged by size: 3079 1019 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 2455 3260 2455 Size: 805 5130 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 2460 5811 2460 Size: 3351 2584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5049 5233 1305 Size: 3744 184 2007 Fragments arranged by size: 3744 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4113 Fragments arranged by size: 4113 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2681 3120 3893 4252 4411 518 Size: 648 1515 439 773 359 159 2042 Fragments arranged by size: 2042 1515 773 648 439 359 159 MspA1I CmG'CkG Cuts at: 1606 2569 2664 2917 2929 2974 3351 4028 5176 Size: 963 95 253 12 45 377 677 1148 Cuts at: 5176 5421 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 963 677 377 253 245 95 45 12 NaeI GCC'GGC Cuts at: 2747 2747 Size: 5935 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5383 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NgoMIV G'CCGG_C Cuts at: 2745 2745 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NruI TCG'CGA Cuts at: 3181 3181 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 3293 4838 1132 Size: 97 2064 1545 2229 Fragments arranged by size: 2229 2064 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5935 PfoI T'CCnGG_A Cuts at: 2598 2730 2598 Size: 132 5803 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PmlI CAC'GTG Cuts at: 2359 2359 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2989 2989 Size: 5935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PspOMI G'GGCC_C Cuts at: 2025 2120 3030 2025 Size: 95 910 4930 Fragments arranged by size: 4930 910 95 PstI C_TGCA'G Cuts at: 830 2979 3287 830 Size: 2149 308 3478 Fragments arranged by size: 3478 2149 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2569 2917 2974 2569 Size: 348 57 5530 Fragments arranged by size: 5530 348 57 SacI G_AGCT'C Cuts at: 2760 2760 Size: 5935 SacII CC_GC'GG Cuts at: 2930 2930 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SapI GCTCTTCn'nnn_ Cuts at: 2600 2600 Size: 5935 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 2204 4183 5895 2204 Size: 1979 1712 2244 Fragments arranged by size: 2244 1979 1712 SexAI A'CCwGG_T Cuts at: 2055 2847 2055 Size: 792 5143 SfcI C'TryA_G Cuts at: 826 1045 2188 2515 2975 3187 3283 4418 5099 Size: 219 1143 327 460 212 96 1135 681 Cuts at: 5099 5290 826 Size: 191 1471 Fragments arranged by size: 1471 1143 1135 681 460 327 219 212 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4039 4940 5202 820 Size: 197 926 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 2068 1517 Size: 551 5384 StyI C'CwwG_G Cuts at: 513 1065 2073 3222 3374 513 Size: 552 1008 1149 152 3074 Fragments arranged by size: 3074 1149 1008 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 3962 3979 4132 4317 4762 325 Size: 1255 1482 900 17 153 185 445 1498 Fragments arranged by size: 1498 1482 1255 900 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 4181 5893 Size: 274 80 33 51 1443 225 1979 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1979 1712 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 2726 2758 5895 5927 2189 Size: 32 505 32 3137 32 2197 Fragments arranged by size: 3137 2197 505 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2462 3229 2462 Size: 767 5168 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 4064 4064 Size: 5935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3163 3743 276 Size: 53 83 186 936 1629 580 2468 Fragments arranged by size: 2468 1629 936 580 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NsiI PacI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI