(Linear) (Six-base) MAPSORT of: KIBB7768.seq Check: 1764 from: 1 to: 1230 KIBB7768.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 298 1230 Size: 298 932 AatII G_ACGT'C Cuts at: 0 1160 1230 Size: 1160 70 Acc65I G'GTAC_C Cuts at: 0 446 1230 Size: 446 784 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 119 290 313 1230 Size: 65 54 171 23 917 Fragments arranged by size: 917 171 65 54 23 AflIII A'CryG_T Cuts at: 0 455 1230 Size: 455 775 AleI CACnn'nnGTG Cuts at: 0 1115 1230 Size: 1115 115 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 520 552 1230 Size: 520 32 678 Fragments arranged by size: 678 520 32 AlwNI CAG_nnn'CTG Cuts at: 0 57 147 518 1230 Size: 57 90 371 712 Fragments arranged by size: 712 371 90 57 ApaI G_GGCC'C Cuts at: 0 24 119 1029 1230 Size: 24 95 910 201 Fragments arranged by size: 910 201 95 24 AvaI C'yCGr_G Cuts at: 0 523 1230 Size: 523 707 BanI G'GyrC_C Cuts at: 0 446 1230 Size: 446 784 BanII G_rGCy'C Cuts at: 0 24 119 629 755 1029 1230 Size: 24 95 510 126 274 201 Fragments arranged by size: 510 274 201 126 95 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 53 1230 Size: 53 1177 BbvCI CC'TCA_GC Cuts at: 0 58 1230 Size: 58 1172 BlpI GC'TnA_GC Cuts at: 0 623 1230 Size: 623 607 Bme1580I G_kGCm'C Cuts at: 0 24 119 1029 1191 1230 Size: 24 95 910 162 39 Fragments arranged by size: 910 162 95 39 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 769 882 1230 Size: 586 183 113 348 Fragments arranged by size: 586 348 183 113 Bpu10I CC'TnA_GC Cuts at: 0 58 652 697 1230 Size: 58 594 45 533 Fragments arranged by size: 594 533 58 45 BsaI GGTCTCn'nnnn_ Cuts at: 0 947 1128 1230 Size: 947 181 102 Fragments arranged by size: 947 181 102 BsaAI yAC'GTr Cuts at: 0 354 1230 Size: 354 876 BsaHI Gr'CG_yC Cuts at: 0 497 573 666 689 1157 1230 Size: 497 76 93 23 468 73 Fragments arranged by size: 497 468 93 76 73 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 49 184 214 721 751 1230 Size: 19 30 135 30 507 30 479 Fragments arranged by size: 507 479 135 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 306 1230 Size: 306 924 BseYI C'CCAG_C Cuts at: 0 1128 1230 Size: 1128 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 732 928 1230 Size: 78 654 196 302 Fragments arranged by size: 654 302 196 78 BsiEI CG_ry'CG Cuts at: 0 603 1230 Size: 603 627 BsiHKAI G_wGCw'C Cuts at: 0 755 1230 Size: 755 475 BsmBI CGTCTCn'nnnn_ Cuts at: 0 433 510 1164 1230 Size: 433 77 654 66 Fragments arranged by size: 654 433 77 66 Bsp1286I G_dGCh'C Cuts at: 0 24 119 629 755 1029 1191 1230 Size: 24 95 510 126 274 162 39 Fragments arranged by size: 510 274 162 126 95 39 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 298 1230 Size: 298 932 BsrBI CCG'CTC Cuts at: 0 570 1230 Size: 570 660 BsrFI r'CCGG_y Cuts at: 0 740 942 1230 Size: 740 202 288 Fragments arranged by size: 740 288 202 BssHII G'CGCG_C Cuts at: 0 618 1230 Size: 618 612 BssSI C'ACGA_G Cuts at: 0 193 1230 Size: 193 1037 BstEII G'GTnAC_C Cuts at: 0 846 1230 Size: 846 384 BstXI CCAn_nnnn'nTGG Cuts at: 0 246 1230 Size: 246 984 BstYI r'GATC_y Cuts at: 0 1164 1230 Size: 1164 66 Bsu36I CC'TnA_GG Cuts at: 0 1016 1055 1230 Size: 1016 39 175 Fragments arranged by size: 1016 175 39 BtgI C'CryG_G Cuts at: 0 922 1230 Size: 922 308 BtsI GCAGTG_nn' Cuts at: 0 120 1230 Size: 120 1110 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 335 370 1230 Size: 335 35 860 Fragments arranged by size: 860 335 35 DraIII CAC_nnn'GTG Cuts at: 0 846 1230 Size: 846 384 DrdI GACnn_nn'nnGTC Cuts at: 0 502 1069 1230 Size: 502 567 161 Fragments arranged by size: 567 502 161 EaeI y'GGCC_r Cuts at: 0 401 1230 Size: 401 829 EarI CTCTTCn'nnn_ Cuts at: 0 332 595 1230 Size: 332 263 635 Fragments arranged by size: 635 332 263 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 621 808 1230 Size: 621 187 422 Fragments arranged by size: 621 422 187 EcoICRI GAG'CTC Cuts at: 0 753 1230 Size: 753 477 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 119 290 313 586 769 882 1230 Size: 65 54 171 23 273 183 113 348 Fragments arranged by size: 348 273 183 171 113 65 54 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 223 1230 Size: 223 1007 EcoO109I rG'GnC_Cy Cuts at: 0 20 21 116 281 732 984 1026 1078 Size: 20 1 95 165 451 252 42 52 Cuts at: 1078 1230 Size: 152 Fragments arranged by size: 451 252 165 152 95 52 42 20 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 175 1230 Size: 175 1055 HaeII r_GCGC'y Cuts at: 0 817 919 1230 Size: 817 102 311 Fragments arranged by size: 817 311 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 19 51 656 688 995 1027 1156 1188 Size: 19 32 605 32 307 32 129 32 Cuts at: 1188 1230 Size: 42 Fragments arranged by size: 605 307 129 42 32 32 32 32 19 HincII GTy'rAC Cuts at: 0 255 1230 Size: 255 975 KpnI G_GTAC'C Cuts at: 0 450 1230 Size: 450 780 MluI A'CGCG_T Cuts at: 0 455 1230 Size: 455 775 MslI CAynn'nnrTG Cuts at: 0 676 1115 1230 Size: 676 439 115 Fragments arranged by size: 676 439 115 MspA1I CmG'CkG Cuts at: 0 564 659 912 924 969 1230 Size: 564 95 253 12 45 261 Fragments arranged by size: 564 261 253 95 45 12 NaeI GCC'GGC Cuts at: 0 742 1230 Size: 742 488 NgoMIV G'CCGG_C Cuts at: 0 740 1230 Size: 740 490 NruI TCG'CGA Cuts at: 0 1176 1230 Size: 1176 54 PfoI T'CCnGG_A Cuts at: 0 593 725 1230 Size: 593 132 505 Fragments arranged by size: 593 505 132 PmlI CAC'GTG Cuts at: 0 354 1230 Size: 354 876 PpuMI rG'GwC_Cy Cuts at: 0 984 1230 Size: 984 246 PspOMI G'GGCC_C Cuts at: 0 20 115 1025 1230 Size: 20 95 910 205 Fragments arranged by size: 910 205 95 20 PstI C_TGCA'G Cuts at: 0 974 1230 Size: 974 256 PvuII CAG'CTG Cuts at: 0 564 912 969 1230 Size: 564 348 57 261 Fragments arranged by size: 564 348 261 57 SacI G_AGCT'C Cuts at: 0 755 1230 Size: 755 475 SacII CC_GC'GG Cuts at: 0 925 1230 Size: 925 305 SapI GCTCTTCn'nnn_ Cuts at: 0 595 1230 Size: 595 635 ScaI AGT'ACT Cuts at: 0 199 1230 Size: 199 1031 SexAI A'CCwGG_T Cuts at: 0 50 842 1230 Size: 50 792 388 Fragments arranged by size: 792 388 50 SfcI C'TryA_G Cuts at: 0 183 510 970 1182 1230 Size: 183 327 460 212 48 Fragments arranged by size: 460 327 212 183 48 StuI AGG'CCT Cuts at: 0 63 1230 Size: 63 1167 StyI C'CwwG_G Cuts at: 0 68 1217 1230 Size: 68 1149 13 Fragments arranged by size: 1149 68 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1057 1230 Size: 1057 173 TatI w'GTAC_w Cuts at: 0 197 1230 Size: 197 1033 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 184 216 721 753 1230 Size: 184 32 505 32 477 Fragments arranged by size: 505 477 184 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 457 1230 Size: 457 773 ZraI GAC'GTC Cuts at: 0 1158 1230 Size: 1158 72 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AleI AloI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MluI MslI MspA1I NaeI NgoMIV NruI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI StuI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstZ17I BtgZI ClaI DraI EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MmeI MscI NarI NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI