################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AfaI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRII EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspA1I MspI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 3 GACGTC GACGT^C AcyI 3 GACGTC G[GA]^CG[CT]C AhdI 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 674 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 395 GGTCC G^G[AT]CC BalI 966 TGGCCA TGG^CCA BbsI 124 GAAGAC GAAGAC(2/6) BcgI 174 CGAATATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 956 GTATCC GTATCC(6/5) BfaI 692 CTAG C^TAG BpuAI 124 GAAGAC GAAGAC(2/6) BsaHI 3 GACGTC G[GA]^CG[CT]C BsaI 930 GGTCTC GGTCTC(1/5) BsaJI 795 CCAAGG C^C[ACGT][ACGT]GG BsiYI 111 CCAAATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 111 CCAAATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 396 GTCCC GGGAC(10/14) BstYI 290 AGATCC [GA]^GATC[CT] Cfr13I 395 GGTCC G^G[ACGT]CC DraII 394 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 966 TGGCCA [CT]^GGCC[GA] Eam1105I 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 297 GAAGAG CTCTTC(1/4) EcoO109I 394 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 201 GAATTC G^AATTC EcoT14I 795 CCAAGG C^C[AT][AT]GG HaeIII 967 GGCC GG^CC Hin1I 3 GACGTC G[GA]^CG[CT]C Ksp632I 297 GAAGAG CTCTTC(1/4) MaeI 692 CTAG C^TAG MaeII 4 ACGT A^CGT MfeI 423 CAATTG C^AATTG MflI 290 AGATCC [GA]^GATC[CT] MluNI 966 TGGCCA TGG^CCA MscI 966 TGGCCA TGG^CCA MunI 423 CAATTG C^AATTG NlaIV 395 GGTCCC GG[ACGT]^[ACGT]CC NspI 373 GCATGC [GA]CATG^[CT] PflMI 111 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 529 GAGTC GAGTC(4/5) PpuMI 394 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 3 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau96I 395 GGTCC G^G[ACGT]CC SphI 373 GCATGC GCATG^C SspI 19 AATATT AAT^ATT StyI 795 CCAAGG C^C[AT][AT]GG TaqI 173 TCGA T^CGA TthHB8I 173 TCGA T^CGA Van91I 111 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 290 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AlwI 291 GATCC GGATC(4/5) 855 GGATC Asp700I 16 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 159 GAAAAAATTC BpmI 454 CTGGAG CTGGAG(16/14) 893 CTGGAG BsgI 593 CTGCAC GTGCAG(16/14) 1005 CTGCAC DdeI 218 CTCAG C^T[ACGT]AG 1016 CTGAG MaeIII 386 GTGAC ^GT[ACGT]AC 835 GTCAC SfcI 62 CTGTAG C^T[GA][CT]AG 821 CTACAG Tsp45I 386 GTGAC ^GT[GC]AC 835 GTCAC XmnI 16 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 159 GAAAAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AluI ApoI BbvI BsmAI BsrI BstF5I Cac8I DpnII EcoT22I Fnu4HI FokI HinfI HphI ItaI MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NsiI Sau3AI SfaNI TfiI Tru9I TseI Tsp509I TspRI