(Circular) (Six-base) MAPSORT of: pFN21AE6344.seq Check: 7931 from: 1 to: 5770 pFN21AE6344 5770 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2892 2892 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2039 3580 278 Size: 53 83 186 936 503 1541 2468 Fragments arranged by size: 2468 1541 936 503 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2286 2479 2852 3086 3833 5217 5485 Size: 410 244 193 373 234 747 1384 268 Cuts at: 5485 1632 Size: 1917 Fragments arranged by size: 1917 1384 747 410 373 268 244 234 193 AfeI AGC'GCT Cuts at: 2486 2486 Size: 5770 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 3041 1166 Size: 1875 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2438 1184 Size: 1254 4516 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2085 2351 3079 3305 3457 5566 1493 Size: 592 266 728 226 152 2109 1697 Fragments arranged by size: 2109 1697 728 592 266 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2049 3095 1870 Size: 73 106 1046 4545 Fragments arranged by size: 4545 1046 106 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 3091 4546 618 Size: 325 217 627 279 1025 1455 1842 Fragments arranged by size: 1842 1455 1025 627 325 279 217 BanII G_rGCy'C Cuts at: 1391 2968 3089 1391 Size: 1577 121 4072 Fragments arranged by size: 4072 1577 121 BbeI G_GCGC'C Cuts at: 2070 2070 Size: 5770 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2849 2883 3961 3995 1903 Size: 34 912 34 1078 34 3678 Fragments arranged by size: 3678 1078 912 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2634 3831 4987 1790 Size: 844 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 844 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 4430 1239 Size: 1295 1896 2579 Fragments arranged by size: 2579 1896 1295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2778 2840 3895 4760 5058 2778 Size: 62 1055 865 298 3490 Fragments arranged by size: 3490 1055 865 298 62 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 2621 5764 2621 Size: 3143 2627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2067 3577 3959 Size: 53 83 186 936 503 31 1510 382 Cuts at: 3959 4594 275 Size: 635 1451 Fragments arranged by size: 1510 1451 936 635 503 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4411 Fragments arranged by size: 4411 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5770 BseYI C'CCAG_C Cuts at: 2070 4973 5469 2070 Size: 2903 496 2371 Fragments arranged by size: 2903 2371 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2120 1163 Size: 573 384 4813 Fragments arranged by size: 4813 573 384 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 2968 3831 3916 4987 2451 Size: 517 863 85 1071 3234 Fragments arranged by size: 3234 1071 863 517 85 BsmI GAATG_Cn' Cuts at: 2725 2931 3281 3374 2725 Size: 206 350 93 5121 Fragments arranged by size: 5121 350 206 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2634 2968 3089 3831 3916 4987 Size: 399 661 183 334 121 742 85 1071 Cuts at: 4987 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 661 399 334 183 121 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 2892 3125 844 Size: 1763 285 233 3489 Fragments arranged by size: 3489 1763 285 233 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2380 4265 4439 65 Size: 1183 1132 1885 174 1396 Fragments arranged by size: 1885 1396 1183 1132 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4414 4601 1700 Size: 110 2604 187 2869 Fragments arranged by size: 2869 2604 187 110 BsrGI T'GTAC_A Cuts at: 96 2409 2523 96 Size: 2313 114 3343 Fragments arranged by size: 3343 2313 114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2671 3057 3100 3467 3852 3869 5321 5407 Size: 699 386 43 367 385 17 1452 86 Cuts at: 5407 5419 5765 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 699 386 385 367 346 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2849 513 Size: 552 939 845 3434 Fragments arranged by size: 3434 939 845 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2534 487 Size: 683 56 1308 3723 Fragments arranged by size: 3723 1308 683 56 BtsI GCAGTG_nn' Cuts at: 2101 2108 2555 3286 4080 4100 2101 Size: 7 447 731 794 20 3771 Fragments arranged by size: 3771 794 731 447 20 7 ClaI AT'CG_AT Cuts at: 1554 2625 1554 Size: 1071 4699 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2790 2825 529 Size: 35 2226 35 3474 Fragments arranged by size: 3474 2226 35 35 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DraIII CAC_nnn'GTG Cuts at: 2614 2614 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2041 4777 809 Size: 507 725 2736 1802 Fragments arranged by size: 2736 1802 725 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735 Size: 54 1425 50 275 18 1387 889 1629 Cuts at: 5735 8 Size: 43 Fragments arranged by size: 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2570 3700 1353 Size: 1217 1130 3423 Fragments arranged by size: 3423 1217 1130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2035 2668 4357 4731 4877 387 Size: 1064 138 446 633 1689 374 146 1280 Fragments arranged by size: 1689 1280 1064 633 446 374 146 138 EcoICRI GAG'CTC Cuts at: 2966 2966 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2286 2479 2534 2852 3086 3833 Size: 393 410 244 193 55 318 234 747 Cuts at: 3833 4430 5217 5485 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 747 597 410 393 318 268 244 234 193 55 EcoRI G'AATT_C Cuts at: 2085 3079 3457 2085 Size: 994 378 4398 Fragments arranged by size: 4398 994 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2237 2752 2237 Size: 515 5255 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2070 2488 4917 1678 Size: 392 418 2429 2531 Fragments arranged by size: 2531 2429 418 392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2266 2298 4411 4443 4485 Size: 32 135 32 694 32 2113 32 42 Cuts at: 4485 4517 1373 Size: 32 2626 Fragments arranged by size: 2626 2113 694 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KasI G'GCGC_C Cuts at: 2066 2066 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 4884 5068 1305 Size: 1155 2424 184 2007 Fragments arranged by size: 2424 2007 1155 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2627 3041 3728 4087 4246 518 Size: 648 928 533 414 687 359 159 2042 Fragments arranged by size: 2042 928 687 648 533 414 359 159 MspA1I CmG'CkG Cuts at: 1606 3186 3863 5011 5256 1606 Size: 1580 677 1148 245 2120 Fragments arranged by size: 2120 1580 1148 677 245 NarI GG'CG_CC Cuts at: 2067 2067 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 3128 4673 1132 Size: 97 1899 1545 2229 Fragments arranged by size: 2229 1899 1545 97 PasI CC'CwG_GG Cuts at: 1348 3026 1348 Size: 1678 4092 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PfoI T'CCnGG_A Cuts at: 2511 2946 2511 Size: 435 5335 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2400 2430 3122 830 Size: 1570 30 692 3478 Fragments arranged by size: 3478 1570 692 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 SacI G_AGCT'C Cuts at: 2968 2968 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SapI GCTCTTCn'nnn_ Cuts at: 2570 2570 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2432 4018 5730 2432 Size: 1586 1712 2472 Fragments arranged by size: 2472 1712 1586 SexAI A'CCwGG_T Cuts at: 2610 2610 Size: 5770 SfcI C'TryA_G Cuts at: 826 1045 2187 2338 2396 2426 2705 3118 4253 Size: 219 1142 151 58 30 279 413 1135 Cuts at: 4253 4934 5125 826 Size: 681 191 1471 Fragments arranged by size: 1471 1142 1135 681 413 279 219 191 151 58 30 SfoI GGC'GCC Cuts at: 2068 2068 Size: 5770 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 2051 3097 1872 Size: 179 1046 4545 Fragments arranged by size: 4545 1046 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2793 2855 3874 4775 5037 820 Size: 197 926 850 62 1019 901 262 1553 Fragments arranged by size: 1553 1019 926 901 850 262 197 62 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 3209 513 Size: 552 939 438 767 3074 Fragments arranged by size: 3074 939 767 552 438 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3797 3814 3967 4152 4597 325 Size: 1255 2217 17 153 185 445 1498 Fragments arranged by size: 2217 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2204 2409 2430 Size: 274 80 33 51 1443 227 205 21 Cuts at: 2430 2523 3018 4016 5728 96 Size: 93 495 998 1712 138 Fragments arranged by size: 1712 1443 998 495 274 227 205 138 93 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2917 2949 5730 5762 2917 Size: 32 2781 32 2925 Fragments arranged by size: 2925 2781 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2981 1316 Size: 1665 4105 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 2049 3095 1870 Size: 179 1046 4545 Fragments arranged by size: 4545 1046 179 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2037 3578 276 Size: 53 83 186 936 503 1541 2468 Fragments arranged by size: 2468 1541 936 503 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI