(Circular) (Six-base) MAPSORT of: pFN21AB0983.seq Check: 7816 from: 1 to: 5425 pFN21AB0983 5425 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2287 2746 2287 Size: 459 4966 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3488 4872 5140 1632 Size: 1856 1384 268 1917 Fragments arranged by size: 1917 1856 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2048 1184 Size: 864 4561 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 5393 AlwNI CAG_nnn'CTG Cuts at: 2393 2462 3168 4740 2393 Size: 69 706 1572 3078 Fragments arranged by size: 3078 1572 706 69 ApaI G_GGCC'C Cuts at: 2606 2606 Size: 5425 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2119 2734 2960 3112 5221 1493 Size: 626 615 226 152 2109 1697 Fragments arranged by size: 2109 1697 626 615 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2628 2750 1870 Size: 73 340 345 122 4545 Fragments arranged by size: 4545 345 340 122 73 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2287 2746 4201 618 Size: 325 217 627 303 197 459 1455 1842 Fragments arranged by size: 1842 1455 627 459 325 303 217 197 BanII G_rGCy'C Cuts at: 1391 2497 2606 2744 1391 Size: 1106 109 138 4072 Fragments arranged by size: 4072 1106 138 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3616 3650 1903 Size: 34 1679 34 3678 Fragments arranged by size: 3678 1679 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2072 2248 3317 4527 2072 Size: 176 1069 1210 2970 Fragments arranged by size: 2970 1210 1069 176 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4036 136 Size: 107 122 71 631 605 2364 1525 Fragments arranged by size: 2364 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2525 2606 3486 4642 1790 Size: 735 81 880 1156 2573 Fragments arranged by size: 2573 1156 880 735 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2688 2688 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 2328 2511 2544 4085 1239 Size: 1080 9 183 33 1541 2579 Fragments arranged by size: 2579 1541 1080 183 33 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2584 2659 4088 882 Size: 617 1085 75 1429 2219 Fragments arranged by size: 2219 1429 1085 617 75 BsaAI yAC'GTr Cuts at: 493 1204 3216 493 Size: 711 2012 2702 Fragments arranged by size: 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2636 3232 3614 4249 Size: 53 83 186 936 1103 596 382 635 Cuts at: 4249 275 Size: 1451 Fragments arranged by size: 1451 1103 936 635 596 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2542 2572 1178 Size: 30 165 30 1139 30 4031 Fragments arranged by size: 4031 1139 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2355 2415 2565 1186 Size: 1169 60 150 4046 Fragments arranged by size: 4046 1169 150 60 BseYI C'CCAG_C Cuts at: 2418 4628 5124 2418 Size: 2210 496 2719 Fragments arranged by size: 2719 2210 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4852 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 3636 4664 1540 Size: 275 186 874 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 3571 4642 3486 Size: 85 1071 4269 Fragments arranged by size: 4269 1071 85 BsmI GAATG_Cn' Cuts at: 2173 2712 2936 3029 2173 Size: 539 224 93 4569 Fragments arranged by size: 4569 539 224 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2497 2525 2606 2744 3486 3571 4642 Size: 399 707 28 81 138 742 85 1071 Cuts at: 4642 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 707 399 138 85 81 28 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2413 2780 844 Size: 1569 367 3489 Fragments arranged by size: 3489 1569 367 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 3920 4094 65 Size: 1183 1029 1643 174 1396 Fragments arranged by size: 1643 1396 1183 1029 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4069 4256 1700 Size: 110 2259 187 2869 Fragments arranged by size: 2869 2259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 2462 794 Size: 1668 3757 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5425 BstYI r'GATC_y Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420 Size: 783 367 385 17 1452 86 12 346 Cuts at: 5420 1972 Size: 1977 Fragments arranged by size: 1977 1452 783 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2598 513 Size: 552 1533 3340 Fragments arranged by size: 3340 1533 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2941 3735 3755 2941 Size: 794 20 4611 Fragments arranged by size: 4611 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2872 3761 Size: 54 1425 50 275 18 244 798 889 Cuts at: 3761 5390 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 798 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2437 3355 1353 Size: 1084 918 3423 Fragments arranged by size: 3423 1084 918 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2517 4012 4386 4532 387 Size: 1064 138 928 1495 374 146 1280 Fragments arranged by size: 1495 1280 1064 928 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2328 2511 2544 3488 4085 4872 Size: 393 687 9 183 33 944 597 787 Cuts at: 4872 5140 1239 Size: 268 1524 Fragments arranged by size: 1524 944 787 687 597 393 268 183 33 9 EcoNI CCTnn'n_nnAGG Cuts at: 2397 2628 2676 2700 2397 Size: 231 48 24 5122 Fragments arranged by size: 5122 231 48 24 EcoO109I rG'GnC_Cy Cuts at: 2312 2371 2390 2312 Size: 59 19 5347 Fragments arranged by size: 5347 59 19 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 4572 1678 Size: 2894 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373 Size: 32 135 32 2494 32 42 32 2626 Fragments arranged by size: 2626 2494 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2291 2750 2291 Size: 459 4966 MfeI C'AATT_G Cuts at: 2610 3024 2610 Size: 414 5011 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4539 4723 1305 Size: 3234 184 2007 Fragments arranged by size: 3234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3603 Fragments arranged by size: 3603 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 3383 3742 3901 518 Size: 648 1080 1137 359 159 2042 Fragments arranged by size: 2042 1137 1080 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2333 2351 2459 2841 3518 4666 4911 1606 Size: 727 18 108 382 677 1148 245 2120 Fragments arranged by size: 2120 1148 727 677 382 245 108 18 NcoI C'CATG_G Cuts at: 513 1065 2598 513 Size: 552 1533 3340 Fragments arranged by size: 3340 1533 552 NdeI CA'TA_TG Cuts at: 387 2115 387 Size: 1728 3697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2650 2783 4328 1132 Size: 97 1421 133 1545 2229 Fragments arranged by size: 2229 1545 1421 133 97 PasI CC'CwG_GG Cuts at: 1348 2315 1348 Size: 967 4458 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 2612 1108 Size: 1189 315 3921 Fragments arranged by size: 3921 1189 315 PfoI T'CCnGG_A Cuts at: 2656 2656 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PspOMI G'GGCC_C Cuts at: 2602 2602 Size: 5425 PspXI vC'TCGA_Gb Cuts at: 2628 2628 Size: 5425 PstI C_TGCA'G Cuts at: 830 2037 2777 830 Size: 1207 740 3478 Fragments arranged by size: 3478 1207 740 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 PvuII CAG'CTG Cuts at: 2333 2351 2459 2333 Size: 18 108 5299 Fragments arranged by size: 5299 108 18 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SexAI A'CCwGG_T Cuts at: 2581 2581 Size: 5425 SfcI C'TryA_G Cuts at: 826 1045 2033 2047 2451 2773 3908 4589 4780 Size: 219 988 14 404 322 1135 681 191 Cuts at: 4780 826 Size: 1471 Fragments arranged by size: 1471 1135 988 681 404 322 219 191 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2752 1872 Size: 880 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2628 3529 4430 4692 820 Size: 197 926 685 901 901 262 1553 Fragments arranged by size: 1553 926 901 901 685 262 197 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2650 2783 1132 Size: 1518 133 3774 Fragments arranged by size: 3774 1518 133 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5425 StyI C'CwwG_G Cuts at: 513 1065 2598 2864 513 Size: 552 1533 266 3074 Fragments arranged by size: 3074 1533 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 3452 3469 3622 3807 4252 325 Size: 1255 1031 841 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1031 841 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3671 5383 96 Size: 274 80 33 51 1443 1694 1712 138 Fragments arranged by size: 1712 1694 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5385 5417 5385 Size: 32 5393 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2375 2375 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 2628 1943 Size: 685 4740 XmaI C'CCGG_G Cuts at: 1870 2750 1870 Size: 880 4545 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI