Restriction map for
pFN21AE6301
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AlwI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I ClaI DpnII
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HindIII KasI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MunI MvnI NarI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacI SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 895 GTCTAC GT^[AC][GT]AC
AccIII 1211 TCCGGA T^CCGGA
AcsI 963 GAATTT [GA]^AATT[CT]
Alw44I 421 GTGCAC G^TGCAC
AlwNI 241 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 468 GGGCCC GGGCC^C
ApaLI 421 GTGCAC G^TGCAC
ApoI 963 GAATTT [GA]^AATT[CT]
AspHI 421 GTGCAC G[AT]GC[AT]^C
AviII 852 TGCGCA TGC^GCA
BalI 566 TGGCCA TGG^CCA
BbsI 144 GAAGAC GAAGAC(2/6)
BbvCI 340 GCTGAGG CCTCAGC(-5/-2)
BlpI 1017 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1017 GCTCAGC GC^T[ACGT]AGC
BpuAI 144 GAAGAC GAAGAC(2/6)
BseAI 1211 TCCGGA T^CCGGA
BsiHKAI 421 GTGCAC G[AT]GC[AT]^C
BsmBI 691 CGTCTC CGTCTC(1/5)
BsmFI 476 GTCCC GGGAC(10/14)
BsmI 543 GAATGC GAATGC(1/-1)
BspEI 1211 TCCGGA T^CCGGA
BspHI 332 TCATGA T^CATGA
BsrBI 1015 CCGCTC CCGCTC(-3/-3)
BssSI 402 CTCGTG CACGAG(-5/-1)
CelII 1017 GCTCAGC GC^T[ACGT]AGC
CpoI 13 CGGTCCG CG^G[AT]CCG
DraIII 424 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 123 CCATGG C^C[GA][CT]GG
FauI 4 GCGGG CCCGC(4/6)
FspI 852 TGCGCA TGC^GCA
HgaI 690 GCGTC GACGC(5/10)
HincII 201 GTTAAC GT[CT]^[GA]AC
HindII 201 GTTAAC GT[CT]^[GA]AC
HpaI 201 GTTAAC GTT^AAC
MaeII 1244 ACGT A^CGT
MluNI 566 TGGCCA TGG^CCA
MroI 1211 TCCGGA T^CCGGA
MscI 566 TGGCCA TGG^CCA
MseI 202 TTAA T^TAA
MslI 1134 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 698 GCCGGC GCC^GGC
NcoI 123 CCATGG C^CATGG
NgoMIV 698 GCCGGC G^CCGGC
PflMI 1088 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 158 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1111 CAGCTG CAG^CTG
RcaI 332 TCATGA T^CATGA
RsrII 13 CGGTCCG CG^G[AT]CCG
SexAI 62 ACCTGGT A^CC[AT]GGT
Tru9I 202 TTAA T^TAA
Tsp45I 998 GTGAC ^GT[GC]AC
Van91I 1088 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Aor51HI 254 AGCGCT AGC^GCT
383 AGCGCT
BanII 468 GGGCCC G[GA]GC[CT]^C
1075 GAGCCC
BglI 346 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1022 GCCTGCCTGGC
BsaWI 323 ACCGGA [AT]^CCGG[AT]
1211 TCCGGA
BsrFI 287 ACCGGC [GA]^CCGG[CT]
698 GCCGGC
BstAPI 423 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
645 GCACGGGCTGC
Cfr10I 287 ACCGGC [GA]^CCGG[CT]
698 GCCGGC
EaeI 566 TGGCCA [CT]^GGCC[GA]
1012 TGGCCG
Eco47III 254 AGCGCT AGC^GCT
383 AGCGCT
HaeII 254 AGCGCT [GA]GCGC^[CT]
383 AGCGCT
MaeIII 106 GTTAC ^GT[ACGT]AC
998 GTGAC
MspA1I 1041 CCGCTG C[AC]G^C[GT]G
1111 CAGCTG
PstI 1051 CTGCAG CTGCA^G
1127 CTGCAG
TfiI 734 GAATC G^A[AT]TC
955 GATTC
Tsp509I 551 AATT ^AATT
964 AATT
XcmI 948 CCAGGAAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1242 CCACGTCGCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI