(Circular) (Six-base) MAPSORT of: pFN21AE6301.seq Check: 6564 from: 1 to: 5974 pFN21AE6301 5974 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2901 3317 1937 Size: 964 416 4594 Fragments arranged by size: 4594 964 416 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 2701 2753 4037 5421 5689 1632 Size: 848 221 52 1284 1384 268 1917 Fragments arranged by size: 1917 1384 1284 848 268 221 52 AfeI AGC'GCT Cuts at: 2261 2390 2261 Size: 129 5845 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 3143 1166 Size: 1977 3997 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2251 3717 5289 2251 Size: 1466 1572 2936 Fragments arranged by size: 2936 1572 1466 ApaI G_GGCC'C Cuts at: 2477 2477 Size: 5974 ApaLI G'TGCA_C Cuts at: 2426 4031 5187 2426 Size: 1605 1156 3213 Fragments arranged by size: 3213 1605 1156 ApoI r'AATT_y Cuts at: 1493 2968 3283 3509 3661 5770 1493 Size: 1475 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2510 2543 2510 Size: 33 5941 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2477 3084 3293 1391 Size: 1086 607 209 4072 Fragments arranged by size: 4072 1086 607 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 928 Size: 971 257 4746 Fragments arranged by size: 4746 971 257 BbvCI CC'TCA_GC Cuts at: 2346 2346 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 3033 4585 Size: 107 122 71 631 605 685 676 1552 Cuts at: 4585 136 Size: 1525 Fragments arranged by size: 1552 1525 685 676 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3023 3391 3023 Size: 368 5606 Bme1580I G_kGCm'C Cuts at: 1790 2430 2439 2477 3093 4035 5191 1790 Size: 640 9 38 616 942 1156 2573 Fragments arranged by size: 2573 1156 942 640 616 38 9 BmgBI CAC'GTC Cuts at: 1318 3250 1318 Size: 1932 4042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2242 2249 3705 Size: 416 53 83 399 24 825 7 1456 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1456 958 825 416 399 83 53 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2047 2624 4634 1239 Size: 791 17 577 2010 2579 Fragments arranged by size: 2579 2010 791 577 17 Bpu10I CC'TnA_GC Cuts at: 2346 2346 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2127 2501 2600 4637 882 Size: 617 628 374 99 2037 2219 Fragments arranged by size: 2219 2037 628 617 374 99 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2328 3216 4407 4805 5079 5226 5637 2328 Size: 888 1191 398 274 147 411 2665 Fragments arranged by size: 2665 1191 888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3176 3191 1186 Size: 847 1143 15 3969 Fragments arranged by size: 3969 1143 847 15 BseYI C'CCAG_C Cuts at: 2829 3228 5177 5673 2829 Size: 399 1949 496 3130 Fragments arranged by size: 3130 1949 496 399 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2430 4035 4120 5191 2430 Size: 1605 85 1071 3213 Fragments arranged by size: 3213 1605 1071 85 BsmI GAATG_Cn' Cuts at: 2554 3485 3578 2554 Size: 931 93 4950 Fragments arranged by size: 4950 931 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2702 2702 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2439 2477 3084 3093 3293 4035 Size: 399 640 9 38 607 9 200 742 Cuts at: 4035 4120 5191 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 607 399 200 85 38 9 9 BspEI T'CCGG_A Cuts at: 3216 5637 3216 Size: 2421 3553 BspHI T'CATG_A Cuts at: 2337 3863 5593 2337 Size: 1526 1730 2718 Fragments arranged by size: 2718 1730 1526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3022 3861 1394 Size: 429 1199 839 3507 Fragments arranged by size: 3507 1199 839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2292 2703 4618 4805 1700 Size: 110 482 411 1915 187 2869 Fragments arranged by size: 2869 1915 482 411 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 2407 4034 5046 2407 Size: 1627 1012 3335 Fragments arranged by size: 3335 1627 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 2656 794 Size: 1640 222 4112 Fragments arranged by size: 4112 1640 222 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2198 2845 3236 2198 Size: 647 391 4936 Fragments arranged by size: 4936 647 391 BstYI r'GATC_y Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969 Size: 1332 367 385 17 1452 86 12 346 Cuts at: 5969 1972 Size: 1977 Fragments arranged by size: 1977 1452 1332 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2128 513 Size: 552 939 124 4359 Fragments arranged by size: 4359 939 552 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 3053 3490 4284 4304 3053 Size: 437 794 20 4723 Fragments arranged by size: 4723 794 437 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2434 2434 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 3017 3421 Size: 54 1425 50 275 18 741 446 404 Cuts at: 3421 4310 5939 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 741 446 404 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 2639 2738 3185 3218 3904 1353 Size: 1019 267 99 447 33 686 3423 Fragments arranged by size: 3423 1019 686 447 267 99 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2768 4561 4935 5081 387 Size: 1064 138 1179 1793 374 146 1280 Fragments arranged by size: 1793 1280 1179 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2047 2480 2624 2701 2753 4037 Size: 393 398 17 433 144 77 52 1284 Cuts at: 4037 4634 5421 5689 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1284 787 597 433 398 393 268 144 77 52 17 EcoO109I rG'GnC_Cy Cuts at: 2164 2248 3010 2164 Size: 84 762 5128 Fragments arranged by size: 5128 762 84 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 2859 4480 2859 Size: 1621 4353 HaeII r_GCGC'y Cuts at: 1678 2263 2392 5121 1678 Size: 585 129 2729 2531 Fragments arranged by size: 2729 2531 585 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4615 4647 4689 4721 1373 Size: 32 135 32 3043 32 42 32 2626 Fragments arranged by size: 3043 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2208 3318 3564 669 Size: 988 281 270 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 270 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 2208 3564 2208 Size: 1356 4618 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 10 Size: 54 1425 343 741 3411 Fragments arranged by size: 3411 1425 741 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3932 4291 4450 518 Size: 648 1977 789 359 159 2042 Fragments arranged by size: 2042 1977 789 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3048 3118 3390 4067 5215 5460 1606 Size: 1442 70 272 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 272 245 70 NaeI GCC'GGC Cuts at: 2705 2705 Size: 5974 NcoI C'CATG_G Cuts at: 513 1065 2004 2128 513 Size: 552 939 124 4359 Fragments arranged by size: 4359 939 552 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 2703 2703 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 2191 2670 1348 Size: 843 479 4652 Fragments arranged by size: 4652 843 479 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 3099 1108 Size: 1991 3983 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2473 2473 Size: 5974 PstI C_TGCA'G Cuts at: 830 3060 3136 3326 830 Size: 2230 76 190 3478 Fragments arranged by size: 3478 2230 190 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 3118 3118 Size: 5974 RsrII CG'GwC_CG Cuts at: 2019 2019 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SexAI A'CCwGG_T Cuts at: 2067 2067 Size: 5974 SfcI C'TryA_G Cuts at: 826 1045 2225 2498 3056 3132 3322 4457 5138 Size: 219 1180 273 558 76 190 1135 681 Cuts at: 5138 5329 826 Size: 191 1471 Fragments arranged by size: 1471 1180 1135 681 558 273 219 191 190 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4078 4979 5241 820 Size: 197 926 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2128 2160 2211 3093 3156 3413 Size: 552 939 124 32 51 882 63 257 Cuts at: 3413 513 Size: 3074 Fragments arranged by size: 3074 939 882 552 257 124 63 51 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 4001 4018 4171 4356 4801 325 Size: 1255 522 1899 17 153 185 445 1498 Fragments arranged by size: 1899 1498 1255 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2985 4220 5932 Size: 274 80 33 51 1443 1008 1235 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 1712 1443 1235 1008 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2986 3018 5934 5966 2986 Size: 32 2916 32 2994 Fragments arranged by size: 2994 2916 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2960 3254 2960 Size: 294 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SanDI SapI SfiI SfoI SrfI SwaI