(Linear) (Six-base) MAPSORT of: KIEE6301.seq Check: 6452 from: 1 to: 1269 KIEE6301.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 896 1269 Size: 896 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 696 748 1269 Size: 475 221 52 521 Fragments arranged by size: 521 475 221 52 AfeI AGC'GCT Cuts at: 0 256 385 1269 Size: 256 129 884 Fragments arranged by size: 884 256 129 AleI CACnn'nnGTG Cuts at: 0 1138 1269 Size: 1138 131 AlwNI CAG_nnn'CTG Cuts at: 0 246 1269 Size: 246 1023 ApaI G_GGCC'C Cuts at: 0 472 1269 Size: 472 797 ApaLI G'TGCA_C Cuts at: 0 421 1269 Size: 421 848 ApoI r'AATT_y Cuts at: 0 963 1269 Size: 963 306 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 505 538 1269 Size: 505 33 731 Fragments arranged by size: 731 505 33 BanII G_rGCy'C Cuts at: 0 472 1079 1269 Size: 472 607 190 Fragments arranged by size: 607 472 190 BbsI GAAGACnn'nnnn_ Cuts at: 0 151 1269 Size: 151 1118 BbvCI CC'TCA_GC Cuts at: 0 341 1269 Size: 341 928 BglI GCCn_nnn'nGGC Cuts at: 0 352 1028 1269 Size: 352 676 241 Fragments arranged by size: 676 352 241 BlpI GC'TnA_GC Cuts at: 0 1018 1269 Size: 1018 251 Bme1580I G_kGCm'C Cuts at: 0 425 434 472 1088 1269 Size: 425 9 38 616 181 Fragments arranged by size: 616 425 181 38 9 BmgBI CAC'GTC Cuts at: 0 1245 1269 Size: 1245 24 BmrI ACTGGGnnnn_n' Cuts at: 0 237 244 1269 Size: 237 7 1025 Fragments arranged by size: 1025 237 7 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 42 619 1269 Size: 25 17 577 650 Fragments arranged by size: 650 577 25 17 Bpu10I CC'TnA_GC Cuts at: 0 341 1269 Size: 341 928 BsaI GGTCTCn'nnnn_ Cuts at: 0 122 496 595 1269 Size: 122 374 99 674 Fragments arranged by size: 674 374 122 99 BsaWI w'CCGG_w Cuts at: 0 323 1211 1269 Size: 323 888 58 Fragments arranged by size: 888 323 58 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 1171 1186 1269 Size: 28 1143 15 83 Fragments arranged by size: 1143 83 28 15 BseYI C'CCAG_C Cuts at: 0 824 1223 1269 Size: 824 399 46 Fragments arranged by size: 824 399 46 BsiHKAI G_wGCw'C Cuts at: 0 425 1269 Size: 425 844 BsmI GAATG_Cn' Cuts at: 0 549 1269 Size: 549 720 BsmBI CGTCTCn'nnnn_ Cuts at: 0 697 1269 Size: 697 572 Bsp1286I G_dGCh'C Cuts at: 0 425 434 472 1079 1088 1269 Size: 425 9 38 607 9 181 Fragments arranged by size: 607 425 181 38 9 9 BspEI T'CCGG_A Cuts at: 0 1211 1269 Size: 1211 58 BspHI T'CATG_A Cuts at: 0 332 1269 Size: 332 937 BsrBI CCG'CTC Cuts at: 0 1017 1269 Size: 1017 252 BsrFI r'CCGG_y Cuts at: 0 287 698 1269 Size: 287 411 571 Fragments arranged by size: 571 411 287 BssSI C'ACGA_G Cuts at: 0 402 1269 Size: 402 867 BstAPI GCAn_nnn'nTGC Cuts at: 0 429 651 1269 Size: 429 222 618 Fragments arranged by size: 618 429 222 BstXI CCAn_nnnn'nTGG Cuts at: 0 193 840 1231 1269 Size: 193 647 391 38 Fragments arranged by size: 647 391 193 38 BtgI C'CryG_G Cuts at: 0 123 1269 Size: 123 1146 BtsI GCAGTG_nn' Cuts at: 0 1048 1269 Size: 1048 221 DraIII CAC_nnn'GTG Cuts at: 0 429 1269 Size: 429 840 EaeI y'GGCC_r Cuts at: 0 566 1012 1269 Size: 566 446 257 Fragments arranged by size: 566 446 257 EarI CTCTTCn'nnn_ Cuts at: 0 367 634 733 1180 1213 1269 Size: 367 267 99 447 33 56 Fragments arranged by size: 447 367 267 99 56 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 763 1269 Size: 763 506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 42 475 619 696 748 1269 Size: 25 17 433 144 77 52 521 Fragments arranged by size: 521 433 144 77 52 25 17 EcoO109I rG'GnC_Cy Cuts at: 0 159 243 1005 1269 Size: 159 84 762 264 Fragments arranged by size: 762 264 159 84 FspI TGC'GCA Cuts at: 0 854 1269 Size: 854 415 HaeII r_GCGC'y Cuts at: 0 258 387 1269 Size: 258 129 882 Fragments arranged by size: 882 258 129 HincII GTy'rAC Cuts at: 0 203 1269 Size: 203 1066 HpaI GTT'AAC Cuts at: 0 203 1269 Size: 203 1066 MscI TGG'CCA Cuts at: 0 568 1269 Size: 568 701 MslI CAynn'nnrTG Cuts at: 0 1138 1269 Size: 1138 131 MspA1I CmG'CkG Cuts at: 0 1043 1113 1269 Size: 1043 70 156 Fragments arranged by size: 1043 156 70 NaeI GCC'GGC Cuts at: 0 700 1269 Size: 700 569 NcoI C'CATG_G Cuts at: 0 123 1269 Size: 123 1146 NgoMIV G'CCGG_C Cuts at: 0 698 1269 Size: 698 571 PasI CC'CwG_GG Cuts at: 0 186 665 1269 Size: 186 479 604 Fragments arranged by size: 604 479 186 PflMI CCAn_nnn'nTGG Cuts at: 0 1094 1269 Size: 1094 175 PpuMI rG'GwC_Cy Cuts at: 0 159 1269 Size: 159 1110 PspOMI G'GGCC_C Cuts at: 0 468 1269 Size: 468 801 PstI C_TGCA'G Cuts at: 0 1055 1131 1269 Size: 1055 76 138 Fragments arranged by size: 1055 138 76 PvuII CAG'CTG Cuts at: 0 1113 1269 Size: 1113 156 RsrII CG'GwC_CG Cuts at: 0 14 1269 Size: 14 1255 SexAI A'CCwGG_T Cuts at: 0 62 1269 Size: 62 1207 SfcI C'TryA_G Cuts at: 0 220 493 1051 1127 1269 Size: 220 273 558 76 142 Fragments arranged by size: 558 273 220 142 76 StyI C'CwwG_G Cuts at: 0 123 155 206 1088 1151 1269 Size: 123 32 51 882 63 118 Fragments arranged by size: 882 123 118 63 51 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 97 1269 Size: 97 1172 TatI w'GTAC_w Cuts at: 0 980 1269 Size: 980 289 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 981 1013 1269 Size: 981 32 256 Fragments arranged by size: 981 256 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 955 1249 1269 Size: 955 294 20 Fragments arranged by size: 955 294 20 Enzymes that do cut: AccI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI BaeI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssSI BstAPI BstXI BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FspI HaeII HincII HpaI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PpuMI PspOMI PstI PvuII RsrII SexAI SfcI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BspMI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI