(Circular) (Six-base) MAPSORT of: pFN21AE4467.seq Check: 495 from: 1 to: 6172 pFN21AE4467 6172 bp With 182 enzymes: SgfI * March 28, 2012 14:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2026 2026 Size: 6172 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 2916 3515 1937 Size: 922 57 599 4594 Fragments arranged by size: 4594 922 599 57 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2572 2633 3477 4235 5619 5887 1632 Size: 940 61 844 758 1384 268 1917 Fragments arranged by size: 1917 1384 940 844 758 268 61 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 3213 5071 6048 1203 Size: 2010 1858 977 1327 Fragments arranged by size: 2010 1858 1327 977 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 3364 4901 3364 Size: 1537 4635 AleI CACnn'nnGTG Cuts at: 1166 2213 2285 1166 Size: 1047 72 5053 Fragments arranged by size: 5053 1047 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 2524 2936 3090 3915 5487 2524 Size: 412 154 825 1572 3209 Fragments arranged by size: 3209 1572 825 412 154 ApaI G_GGCC'C Cuts at: 2169 2169 Size: 6172 ApaLI G'TGCA_C Cuts at: 2867 4229 5385 2867 Size: 1362 1156 3654 Fragments arranged by size: 3654 1362 1156 ApoI r'AATT_y Cuts at: 1493 3481 3707 3859 5968 1493 Size: 1988 226 152 2109 1697 Fragments arranged by size: 2109 1988 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2063 3079 3497 1870 Size: 73 120 1016 418 4545 Fragments arranged by size: 4545 1016 418 120 73 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2038 2082 2245 2954 Size: 325 217 627 221 30 44 163 709 Cuts at: 2954 3493 4948 618 Size: 539 1455 1842 Fragments arranged by size: 1842 1455 709 627 539 325 221 217 163 44 30 BanII G_rGCy'C Cuts at: 1391 2070 2169 2307 2376 3189 3491 1391 Size: 679 99 138 69 813 302 4072 Fragments arranged by size: 4072 813 679 302 138 99 69 BbeI G_GCGC'C Cuts at: 2086 2958 2086 Size: 872 5300 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2821 3046 3193 928 Size: 971 922 225 147 3907 Fragments arranged by size: 3907 971 922 225 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3041 4783 136 Size: 107 122 71 631 605 1369 1742 1525 Fragments arranged by size: 1742 1525 1369 631 605 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 2306 3589 2306 Size: 1283 4889 Bme1580I G_kGCm'C Cuts at: 1790 2013 2043 2169 2250 2871 3147 3196 4233 Size: 223 30 126 81 621 276 49 1037 Cuts at: 4233 5389 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1037 621 276 223 126 81 49 30 BmgBI CAC'GTC Cuts at: 1318 2964 3265 1318 Size: 1646 301 4225 Fragments arranged by size: 4225 1646 301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2705 4832 1239 Size: 760 706 2127 2579 Fragments arranged by size: 2579 2127 760 706 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2866 3963 493 Size: 711 1662 1097 2702 Fragments arranged by size: 2702 1662 1097 711 BsaBI GATnn'nnATC Cuts at: 6166 6166 Size: 6172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 2955 3027 3979 Size: 53 83 186 936 550 872 72 952 Cuts at: 3979 4361 4996 275 Size: 382 635 1451 Fragments arranged by size: 1451 952 936 872 635 550 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 2070 4605 5003 5277 5424 5835 2070 Size: 2535 398 274 147 411 2407 Fragments arranged by size: 2535 2407 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5947 Fragments arranged by size: 5947 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6172 BseYI C'CCAG_C Cuts at: 2020 2338 2488 2512 2716 2776 2950 2994 5375 Size: 318 150 24 204 60 174 44 2381 Cuts at: 5375 5871 2020 Size: 496 2321 Fragments arranged by size: 2381 2321 496 318 204 174 150 60 44 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5599 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2653 2798 2841 3622 4383 5411 Size: 275 186 652 145 43 781 761 1028 Cuts at: 5411 1540 Size: 2301 Fragments arranged by size: 2301 1028 781 761 652 275 186 145 43 BsiHKAI G_wGCw'C Cuts at: 2070 2376 2871 3189 4233 4318 5389 2070 Size: 306 495 318 1044 85 1071 2853 Fragments arranged by size: 2853 1071 1044 495 318 306 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2043 2070 2169 2250 2307 2376 Size: 399 223 30 27 99 81 57 69 Cuts at: 2376 2871 3147 3189 3196 3491 4233 4318 5389 Size: 495 276 42 7 295 742 85 1071 Cuts at: 5389 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 495 399 295 276 223 99 85 81 69 57 42 30 27 7 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 4061 5791 4061 Size: 1730 4442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2026 2182 2280 3527 844 Size: 1182 156 98 1247 3489 Fragments arranged by size: 3489 1247 1182 156 98 BsrBI CCG'CTC Cuts at: 1394 1823 4059 1394 Size: 429 2236 3507 Fragments arranged by size: 3507 2236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 2284 2462 2840 4816 5003 1700 Size: 110 402 72 178 378 1976 187 2869 Fragments arranged by size: 2869 1976 402 378 187 178 110 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 2399 2444 2473 2999 1925 Size: 474 45 29 526 5098 Fragments arranged by size: 5098 526 474 45 29 BssSI C'ACGA_G Cuts at: 2087 2432 3140 4232 5244 2087 Size: 345 708 1092 1012 3015 Fragments arranged by size: 3015 1092 1012 708 345 BstAPI GCAn_nnn'nTGC Cuts at: 794 2936 794 Size: 2142 4030 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstXI CCAn_nnnn'nTGG Cuts at: 2950 2950 Size: 6172 BstYI r'GATC_y Cuts at: 1972 2988 3502 3869 4254 4271 5723 5809 5821 Size: 1016 514 367 385 17 1452 86 12 Cuts at: 5821 6167 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 514 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3422 3422 Size: 6172 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2391 2973 3121 3436 513 Size: 552 939 39 348 582 148 315 3249 Fragments arranged by size: 3249 939 582 552 348 315 148 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 3004 3133 487 Size: 683 56 1163 615 129 3526 Fragments arranged by size: 3526 1163 683 615 129 56 BtsI GCAGTG_nn' Cuts at: 3688 4482 4502 3688 Size: 794 20 5358 Fragments arranged by size: 5358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DraIII CAC_nnn'GTG Cuts at: 2350 2350 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 2235 2394 Size: 54 1425 50 275 18 299 106 159 Cuts at: 2394 2460 2795 2838 3033 3619 4508 6137 8 Size: 66 335 43 195 586 889 1629 43 Fragments arranged by size: 1629 1425 889 586 335 299 275 195 159 106 66 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2795 2838 3619 1537 Size: 275 983 43 781 4090 Fragments arranged by size: 4090 983 781 275 43 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 2612 2639 4102 1353 Size: 1028 231 27 1463 3423 Fragments arranged by size: 3423 1463 1028 231 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2374 3187 2068 Size: 306 813 5053 Fragments arranged by size: 5053 813 306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2572 2633 2705 3477 4235 4832 Size: 393 367 573 61 72 772 758 597 Cuts at: 4832 5619 5887 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 772 758 597 573 393 367 268 72 61 EcoO109I rG'GnC_Cy Cuts at: 2165 3271 2165 Size: 1106 5066 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2086 2620 2958 5319 1678 Size: 408 534 338 2361 2531 Fragments arranged by size: 2531 2361 534 408 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2924 2956 3350 Size: 32 135 32 485 32 835 32 394 Cuts at: 3350 3382 4813 4845 4887 4919 1373 Size: 32 1431 32 42 32 2626 Fragments arranged by size: 2626 1431 835 485 394 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3211 3516 3762 669 Size: 988 281 1273 305 246 3079 Fragments arranged by size: 3079 1273 988 305 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 2082 2954 2082 Size: 872 5300 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5286 5470 1305 Size: 3981 184 2007 Fragments arranged by size: 3981 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4350 Fragments arranged by size: 4350 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2285 4130 4489 4648 518 Size: 648 1047 72 1845 359 159 2042 Fragments arranged by size: 2042 1845 1047 648 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2024 2521 2803 2975 2994 3123 3384 3588 Size: 418 497 282 172 19 129 261 204 Cuts at: 3588 4265 5413 5658 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 497 418 282 261 245 204 172 129 19 NaeI GCC'GGC Cuts at: 2464 2842 2464 Size: 378 5794 NarI GG'CG_CC Cuts at: 2083 2955 2083 Size: 872 5300 NcoI C'CATG_G Cuts at: 513 1065 2004 2391 3436 513 Size: 552 939 387 1045 3249 Fragments arranged by size: 3249 1045 939 552 387 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 2462 2840 2462 Size: 378 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 2795 3619 2795 Size: 824 5348 NspI r_CATG'y Cuts at: 1132 1229 2362 3314 3530 5075 1132 Size: 97 1133 952 216 1545 2229 Fragments arranged by size: 2229 1545 1133 952 216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2458 2524 1108 Size: 1242 108 66 4756 Fragments arranged by size: 4756 1242 108 66 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 2165 2165 Size: 6172 PstI C_TGCA'G Cuts at: 830 2301 3524 830 Size: 1471 1223 3478 Fragments arranged by size: 3478 1471 1223 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2521 2803 2521 Size: 282 5890 RsrII CG'GwC_CG Cuts at: 3286 3286 Size: 6172 SacI G_AGCT'C Cuts at: 2070 2376 3189 2070 Size: 306 813 5053 Fragments arranged by size: 5053 813 306 SacII CC_GC'GG Cuts at: 2976 3124 2976 Size: 148 6024 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SapI GCTCTTCn'nnn_ Cuts at: 2381 2381 Size: 6172 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SexAI A'CCwGG_T Cuts at: 2850 2850 Size: 6172 SfcI C'TryA_G Cuts at: 826 1045 2297 2380 3520 4655 5336 5527 826 Size: 219 1252 83 1140 1135 681 191 1471 Fragments arranged by size: 1471 1252 1140 1135 681 219 191 83 SfoI GGC'GCC Cuts at: 2084 2956 2084 Size: 872 5300 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 2212 2284 1700 Size: 512 72 5588 Fragments arranged by size: 5588 512 72 SmaI CCC'GGG Cuts at: 1872 3499 1872 Size: 1627 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6172 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 3436 3611 513 Size: 552 939 387 1045 175 3074 Fragments arranged by size: 3074 1045 939 552 387 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4199 4216 4369 4554 4999 325 Size: 1255 2619 17 153 185 445 1498 Fragments arranged by size: 2619 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2023 2509 2023 Size: 486 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 3497 1870 Size: 1627 4545 XmnI GAAnn'nnTTC Cuts at: 4301 4301 Size: 6172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SanDI SfiI SrfI SwaI