(Linear) (Six-base) MAPSORT of: KIEE4467.seq Check: 9541 from: 1 to: 1467 KIEE4467.orf With 182 enzymes: SgfI * March 22, 2012 18:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 21 1467 Size: 21 1446 AccI GT'mk_AC Cuts at: 0 854 911 1467 Size: 854 57 556 Fragments arranged by size: 854 556 57 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 567 628 1467 Size: 567 61 839 Fragments arranged by size: 839 567 61 AflIII A'CryG_T Cuts at: 0 1208 1467 Size: 1208 259 AhdI GACnn_n'nnGTC Cuts at: 0 1359 1467 Size: 1359 108 AleI CACnn'nnGTG Cuts at: 0 208 280 1467 Size: 208 72 1187 Fragments arranged by size: 1187 208 72 AlwNI CAG_nnn'CTG Cuts at: 0 519 931 1085 1467 Size: 519 412 154 382 Fragments arranged by size: 519 412 382 154 ApaI G_GGCC'C Cuts at: 0 164 1467 Size: 164 1303 ApaLI G'TGCA_C Cuts at: 0 862 1467 Size: 862 605 AvaI C'yCGr_G Cuts at: 0 58 1074 1467 Size: 58 1016 393 Fragments arranged by size: 1016 393 58 BanI G'GyrC_C Cuts at: 0 3 33 77 240 949 1467 Size: 3 30 44 163 709 518 Fragments arranged by size: 709 518 163 44 30 3 BanII G_rGCy'C Cuts at: 0 65 164 302 371 1184 1467 Size: 65 99 138 69 813 283 Fragments arranged by size: 813 283 138 99 69 65 BbeI G_GCGC'C Cuts at: 0 81 953 1467 Size: 81 872 514 Fragments arranged by size: 872 514 81 BbsI GAAGACnn'nnnn_ Cuts at: 0 816 1041 1188 1467 Size: 816 225 147 279 Fragments arranged by size: 816 279 225 147 BglI GCCn_nnn'nGGC Cuts at: 0 1036 1467 Size: 1036 431 BlpI GC'TnA_GC Cuts at: 0 301 1467 Size: 301 1166 Bme1580I G_kGCm'C Cuts at: 0 8 38 164 245 866 1142 1191 1467 Size: 8 30 126 81 621 276 49 276 Fragments arranged by size: 621 276 276 126 81 49 30 8 BmgBI CAC'GTC Cuts at: 0 959 1260 1467 Size: 959 301 207 Fragments arranged by size: 959 301 207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 1467 Size: 700 767 BsaAI yAC'GTr Cuts at: 0 861 1467 Size: 861 606 BsaHI Gr'CG_yC Cuts at: 0 78 950 1022 1467 Size: 78 872 72 445 Fragments arranged by size: 872 445 78 72 BsaWI w'CCGG_w Cuts at: 0 65 1467 Size: 65 1402 BseYI C'CCAG_C Cuts at: 0 15 333 483 507 711 771 945 989 Size: 15 318 150 24 204 60 174 44 Cuts at: 989 1467 Size: 478 Fragments arranged by size: 478 318 204 174 150 60 44 24 15 BsiEI CG_ry'CG Cuts at: 0 648 793 836 1467 Size: 648 145 43 631 Fragments arranged by size: 648 631 145 43 BsiHKAI G_wGCw'C Cuts at: 0 65 371 866 1184 1467 Size: 65 306 495 318 283 Fragments arranged by size: 495 318 306 283 65 Bsp1286I G_dGCh'C Cuts at: 0 8 38 65 164 245 302 371 866 Size: 8 30 27 99 81 57 69 495 Cuts at: 866 1142 1184 1191 1467 Size: 276 42 7 276 Fragments arranged by size: 495 276 276 99 81 69 57 42 30 27 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 21 177 275 1467 Size: 21 156 98 1192 Fragments arranged by size: 1192 156 98 21 BsrFI r'CCGG_y Cuts at: 0 207 279 457 835 1467 Size: 207 72 178 378 632 Fragments arranged by size: 632 378 207 178 72 BssHII G'CGCG_C Cuts at: 0 394 439 468 994 1467 Size: 394 45 29 526 473 Fragments arranged by size: 526 473 394 45 29 BssSI C'ACGA_G Cuts at: 0 82 427 1135 1467 Size: 82 345 708 332 Fragments arranged by size: 708 345 332 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 931 1467 Size: 931 536 BstXI CCAn_nnnn'nTGG Cuts at: 0 945 1467 Size: 945 522 BstYI r'GATC_y Cuts at: 0 983 1467 Size: 983 484 Bsu36I CC'TnA_GG Cuts at: 0 1417 1467 Size: 1417 50 BtgI C'CryG_G Cuts at: 0 38 386 968 1116 1431 1467 Size: 38 348 582 148 315 36 Fragments arranged by size: 582 348 315 148 38 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 384 999 1128 1467 Size: 384 615 129 339 Fragments arranged by size: 615 384 339 129 DraIII CAC_nnn'GTG Cuts at: 0 345 1467 Size: 345 1122 EaeI y'GGCC_r Cuts at: 0 124 230 389 455 790 833 1028 1467 Size: 124 106 159 66 335 43 195 439 Fragments arranged by size: 439 335 195 159 124 106 66 43 EagI C'GGCC_G Cuts at: 0 790 833 1467 Size: 790 43 634 Fragments arranged by size: 790 634 43 EarI CTCTTCn'nnn_ Cuts at: 0 376 607 634 1467 Size: 376 231 27 833 Fragments arranged by size: 833 376 231 27 EcoICRI GAG'CTC Cuts at: 0 63 369 1182 1467 Size: 63 306 813 285 Fragments arranged by size: 813 306 285 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 567 628 700 1467 Size: 567 61 72 767 Fragments arranged by size: 767 567 72 61 EcoO109I rG'GnC_Cy Cuts at: 0 160 1266 1467 Size: 160 1106 201 Fragments arranged by size: 1106 201 160 HaeII r_GCGC'y Cuts at: 0 81 615 953 1467 Size: 81 534 338 514 Fragments arranged by size: 534 514 338 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 52 84 919 951 1345 1377 1467 Size: 52 32 835 32 394 32 90 Fragments arranged by size: 835 394 90 52 32 32 32 HincII GTy'rAC Cuts at: 0 1206 1467 Size: 1206 261 KasI G'GCGC_C Cuts at: 0 77 949 1467 Size: 77 872 518 Fragments arranged by size: 872 518 77 MslI CAynn'nnrTG Cuts at: 0 208 280 1467 Size: 208 72 1187 Fragments arranged by size: 1187 208 72 MspA1I CmG'CkG Cuts at: 0 19 516 798 970 989 1118 1379 1467 Size: 19 497 282 172 19 129 261 88 Fragments arranged by size: 497 282 261 172 129 88 19 19 NaeI GCC'GGC Cuts at: 0 459 837 1467 Size: 459 378 630 Fragments arranged by size: 630 459 378 NarI GG'CG_CC Cuts at: 0 78 950 1467 Size: 78 872 517 Fragments arranged by size: 872 517 78 NcoI C'CATG_G Cuts at: 0 386 1431 1467 Size: 386 1045 36 Fragments arranged by size: 1045 386 36 NgoMIV G'CCGG_C Cuts at: 0 457 835 1467 Size: 457 378 632 Fragments arranged by size: 632 457 378 NotI GC'GGCC_GC Cuts at: 0 790 1467 Size: 790 677 NspI r_CATG'y Cuts at: 0 357 1309 1467 Size: 357 952 158 Fragments arranged by size: 952 357 158 PflMI CCAn_nnn'nTGG Cuts at: 0 345 453 519 1467 Size: 345 108 66 948 Fragments arranged by size: 948 345 108 66 PspOMI G'GGCC_C Cuts at: 0 160 1467 Size: 160 1307 PstI C_TGCA'G Cuts at: 0 296 1467 Size: 296 1171 PvuII CAG'CTG Cuts at: 0 516 798 1467 Size: 516 282 669 Fragments arranged by size: 669 516 282 RsrII CG'GwC_CG Cuts at: 0 1281 1467 Size: 1281 186 SacI G_AGCT'C Cuts at: 0 65 371 1184 1467 Size: 65 306 813 283 Fragments arranged by size: 813 306 283 65 SacII CC_GC'GG Cuts at: 0 971 1119 1467 Size: 971 148 348 Fragments arranged by size: 971 348 148 SapI GCTCTTCn'nnn_ Cuts at: 0 376 1467 Size: 376 1091 SexAI A'CCwGG_T Cuts at: 0 845 1467 Size: 845 622 SfcI C'TryA_G Cuts at: 0 292 375 1467 Size: 292 83 1092 Fragments arranged by size: 1092 292 83 SfoI GGC'GCC Cuts at: 0 79 951 1467 Size: 79 872 516 Fragments arranged by size: 872 516 79 SgrAI Cr'CCGG_yG Cuts at: 0 207 279 1467 Size: 207 72 1188 Fragments arranged by size: 1188 207 72 StyI C'CwwG_G Cuts at: 0 386 1431 1467 Size: 386 1045 36 Fragments arranged by size: 1045 386 36 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 18 504 1467 Size: 18 486 963 Fragments arranged by size: 963 486 18 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI PspOMI PstI PvuII RsrII SacI SacII SapI SexAI SfcI SfoI SgrAI StyI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SfiI SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI