################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 1407 GGCGTC G[GA]^CG[CT]C AspHI 1469 GTGCTC G[AT]GC[AT]^C AvaII 1534 GGACC G^G[AT]CC BamHI 2286 GGATCC G^GATCC BanI 1737 GGCACC G^G[CT][GA]CC BbvCI 1858 CCTCAGC CCTCAGC(-5/-2) BglI 2242 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1046 AGATCT A^GATCT BsaAI 406 TACGTG [CT]AC^GT[GA] BsaHI 1407 GGCGTC G[GA]^CG[CT]C BsaI 251 GGTCTC GGTCTC(1/5) BsaWI 1055 ACCGGA [AT]^CCGG[AT] BsiHKAI 1469 GTGCTC G[AT]GC[AT]^C BsmBI 1386 CGTCTC CGTCTC(1/5) BspLU11I 653 ACATGT A^CATGT BspMI 1989 ACCTGC ACCTGC(4/8) BsrDI 369 CATTGC GCAATG(2/0) BstAPI 1738 GCACCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1442 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 2002 GCGC GCG^C DraI 2215 TTTAAA TTT^AAA DrdI 1332 GACTGCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 697 GATATC GAT^ATC EcoT22I 2195 ATGCAT ATGCA^T FauI 1999 CCCGC CCCGC(4/6) HgaI 1408 GCGTC GACGC(5/10) HhaI 2002 GCGC GCG^C Hin1I 1407 GGCGTC G[GA]^CG[CT]C HinP1I 2002 GCGC G^CGC MaeII 407 ACGT A^CGT NsiI 2195 ATGCAT ATGCA^T NspI 653 ACATGT [GA]CATG^[CT] PflMI 1742 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1435 CTGCAG CTGCA^G SspI 1789 AATATT AAT^ATT Van91I 1742 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 594 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 657 GTCTAC GT^[AC][GT]AC 981 GTCTAC AccII 2001 CGCG CG^CG 2003 CGCG AcsI 741 GAATTC [GA]^AATT[CT] 966 GAATTC AfaI 1307 GTAC GT^AC 1640 GTAC AflIII 407 ACGTGT A^C[GA][CT]GT 653 ACATGT AlwNI 1703 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1799 CAGCTCCTG ApoI 741 GAATTC [GA]^AATT[CT] 966 GAATTC Asp700I 1158 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2205 GAACCCTTTC BbsI 1341 GTCTTC GAAGAC(2/6) 1558 GTCTTC BfaI 595 CTAG C^TAG 1501 CTAG BpuAI 1341 GTCTTC GAAGAC(2/6) 1558 GTCTTC BsaBI 106 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2107 GATGCTAATC BsmAI 252 GTCTC GTCTC(1/5) 1387 GTCTC BsmI 2085 GAATGC GAATGC(1/-1) 2072 GCATTC BstUI 2001 CGCG CG^CG 2003 CGCG DsaI 1087 CCATGG C^C[GA][CT]GG 1537 CCATGG EcoNI 1204 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1525 CCTCAGGCAGG EcoRI 741 GAATTC G^AATTC 966 GAATTC MaeI 595 CTAG C^TAG 1501 CTAG MamI 106 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2107 GATGCTAATC MvnI 2001 CGCG CG^CG 2003 CGCG NcoI 1087 CCATGG C^CATGG 1537 CCATGG PleI 211 GACTC GAGTC(4/5) 1276 GACTC PvuII 242 CAGCTG CAG^CTG 1649 CAGCTG RsaI 1307 GTAC GT^AC 1640 GTAC SfcI 1427 CTACAG C^T[GA][CT]AG 1435 CTGCAG XmnI 1158 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2205 GAACCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI AlwI AocI ApaI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII