################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccIII AclI AfaI AflII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I CelII ClaI CpoI DdeI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 557 AAATTC [GA]^AATT[CT] AcyI 372 GGCGCC G[GA]^CG[CT]C AflIII 392 ACACGT A^C[GA][CT]GT AgeI 152 ACCGGT A^CCGGT ApoI 557 AAATTC [GA]^AATT[CT] AspHI 140 GAGCAC G[AT]GC[AT]^C AspI 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 150 GGACC G^G[AT]CC BanI 372 GGCGCC G^G[CT][GA]CC BbeI 372 GGCGCC GGCGC^C BclI 713 TGATCA T^GATCA BglI 53 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 140 GAGCAC G[AGT]GC[ACT]^C BsaHI 372 GGCGCC G[GA]^CG[CT]C BsaWI 152 ACCGGT [AT]^CCGG[AT] BseRI 93 GAGGAG GAGGAG(10/8) BsiHKAI 140 GAGCAC G[AT]GC[AT]^C Bsp1286I 140 GAGCAC G[AGT]GC[ACT]^C BsrBI 551 GAGCGG CCGCTC(-3/-3) BsrDI 806 GCAATG GCAATG(2/0) Bst1107I 621 GTATAC GTA^TAC BstZ17I 621 GTATAC GTA^TAC DpnII 714 GATC ^GATC DraI 507 TTTAAA TTT^AAA DrdI 412 GACTTCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 57 CCGCGG C^C[GA][CT]GG EaeI 38 TGGCCG [CT]^GGCC[GA] EcoNI 206 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 35 CCTTGG C^C[AT][AT]GG EcoT22I 775 ATGCAT ATGCA^T FauI 68 GCGGG CCCGC(4/6) FbaI 713 TGATCA T^GATCA HaeII 372 GGCGCC [GA]GCGC^[CT] Hin1I 372 GGCGCC G[GA]^CG[CT]C HphI 711 GGTGA GGTGA(8/7) KasI 372 GGCGCC G^GCGCC KspI 57 CCGCGG CCGC^GG MaeII 394 ACGT A^CGT MboI 714 GATC ^GATC MspA1I 57 CCGCGG C[AC]G^C[GT]G NaeI 369 GCCGGC GCC^GGC NarI 372 GGCGCC GG^CGCC NdeII 714 GATC ^GATC NgoMIV 369 GCCGGC G^CCGGC NsiI 775 ATGCAT ATGCA^T NspI 777 GCATGT [GA]CATG^[CT] PflFI 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 152 ACCGGT A^CCGGT PshAI 108 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 57 CCGCGG CCGC^GG Sau3AI 714 GATC ^GATC SfaNI 774 GATGC GCATC(5/9) SfoI 372 GGCGCC GGC^GCC StuI 197 AGGCCT AGG^CCT StyI 35 CCTTGG C^C[AT][AT]GG Tsp509I 558 AATT ^AATT Tth111I 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 424 GTCTAC GT^[AC][GT]AC 621 GTATAC BbsI 690 GAAGAC GAAGAC(2/6) 853 GAAGAC BcnI 110 CCGGG CC^[GC]GG 228 CCCGG BpuAI 690 GAAGAC GAAGAC(2/6) 853 GAAGAC BsgI 135 GTGCAG GTGCAG(16/14) 698 GTGCAG BsmAI 389 GAGAC GTCTC(1/5) 410 GAGAC BsrFI 152 ACCGGT [GA]^CCGG[CT] 369 GCCGGC CfoI 329 GCGC GCG^C 373 GCGC Cfr10I 152 ACCGGT [GA]^CCGG[CT] 369 GCCGGC HhaI 329 GCGC GCG^C 373 GCGC HinP1I 329 GCGC G^CGC 373 GCGC MseI 173 TTAA T^TAA 508 TTAA NciI 110 CCGGG CC^[GC]GG 228 CCCGG PflMI 304 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 753 CCAGGGGATGG TfiI 268 GAATC G^A[AT]TC 476 GAATC Tru9I 173 TTAA T^TAA 508 TTAA Van91I 304 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 753 CCAGGGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI BbvI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NlaIII NlaIV PleI Sau96I ScrFI TseI TspRI