(Circular) (Six-base) MAPSORT of: pFN21AE9258.seq Check: 4139 from: 1 to: 5563 pFN21AE9258 5563 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2627 2906 1937 Size: 493 197 279 4594 Fragments arranged by size: 4594 493 279 197 Acc65I G'GTAC_C Cuts at: 2884 2884 Size: 5563 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 5010 5278 1632 Size: 1994 1384 268 1917 Fragments arranged by size: 1994 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 2397 4462 5439 1203 Size: 1194 2065 977 1327 Fragments arranged by size: 2065 1327 1194 977 AgeI A'CCGG_T Cuts at: 2157 4394 2157 Size: 2237 3326 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5563 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AlwNI CAG_nnn'CTG Cuts at: 3306 4878 3306 Size: 1572 3991 ApaLI G'TGCA_C Cuts at: 3620 4776 3620 Size: 1156 4407 ApoI r'AATT_y Cuts at: 1493 2562 2872 3098 3250 5359 1493 Size: 1069 310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1069 310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2888 1870 Size: 73 945 4545 Fragments arranged by size: 4545 945 73 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 2884 4339 618 Size: 325 217 627 590 507 1455 1842 Fragments arranged by size: 1842 1455 627 590 507 325 217 BanII G_rGCy'C Cuts at: 1391 2882 1391 Size: 1491 4072 BbeI G_GCGC'C Cuts at: 2381 2381 Size: 5563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2702 2865 928 Size: 971 803 163 3626 Fragments arranged by size: 3626 971 803 163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3754 3788 1903 Size: 34 1817 34 3678 Fragments arranged by size: 3678 1817 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 2718 1548 Size: 1170 4393 BfrBI ATG'CAT Cuts at: 2782 2782 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2064 4174 136 Size: 107 122 71 631 605 392 2110 1525 Fragments arranged by size: 2110 1525 631 605 392 122 107 71 BglII A'GATC_T Cuts at: 5558 5558 Size: 5563 BlpI GC'TnA_GC Cuts at: 2980 2980 Size: 5563 Bme1580I G_kGCm'C Cuts at: 1790 3624 4780 1790 Size: 1834 1156 2573 Fragments arranged by size: 2573 1834 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2503 2674 3294 Size: 416 53 83 399 24 1086 171 620 Cuts at: 3294 4252 442 Size: 958 1753 Fragments arranged by size: 1753 1086 958 620 416 399 171 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 2434 2765 4223 1239 Size: 1128 67 331 1458 2579 Fragments arranged by size: 2579 1458 1128 331 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3354 493 Size: 711 2150 2702 Fragments arranged by size: 2702 2150 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2378 3370 3752 4387 Size: 53 83 186 936 845 992 382 635 Cuts at: 4387 275 Size: 1451 Fragments arranged by size: 1451 992 936 845 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2157 3996 4394 4668 4815 5226 2157 Size: 1839 398 274 147 411 2494 Fragments arranged by size: 2494 1839 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 1178 Size: 30 165 30 1003 30 4305 Fragments arranged by size: 4305 1003 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 1186 Size: 927 4636 BseYI C'CCAG_C Cuts at: 4766 5262 4766 Size: 496 5067 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2161 2724 1163 Size: 573 425 563 4002 Fragments arranged by size: 4002 573 563 425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 3624 3709 4780 2149 Size: 1475 85 1071 2932 Fragments arranged by size: 2932 1475 1071 85 BsmI GAATG_Cn' Cuts at: 3074 3167 3074 Size: 93 5470 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2882 3624 3709 4780 1391 Size: 399 359 733 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 399 359 85 BspEI T'CCGG_A Cuts at: 5226 5226 Size: 5563 BspHI T'CATG_A Cuts at: 3452 5182 3452 Size: 1730 3833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 844 Size: 2074 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2558 3450 1394 Size: 429 735 892 3507 Fragments arranged by size: 3507 892 735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2818 4058 4232 65 Size: 1183 1570 1240 174 1396 Fragments arranged by size: 1570 1396 1240 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 2374 4207 4394 1700 Size: 110 347 217 1833 187 2869 Fragments arranged by size: 2869 1833 347 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 3623 4635 3623 Size: 1012 4551 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5563 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558 Size: 921 367 385 17 1452 86 12 346 Cuts at: 5558 1972 Size: 1977 Fragments arranged by size: 1977 1452 921 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2628 2628 Size: 5563 BtgI C'CryG_G Cuts at: 513 1065 2062 513 Size: 552 997 4014 Fragments arranged by size: 4014 997 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4824 Fragments arranged by size: 4824 683 56 BtsI GCAGTG_nn' Cuts at: 3079 3873 3893 3079 Size: 794 20 4749 Fragments arranged by size: 4749 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5528 DraI TTT'AAA Cuts at: 2514 2867 2514 Size: 353 5210 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2423 4570 809 Size: 507 1107 2147 1802 Fragments arranged by size: 2147 1802 1107 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 3010 3899 Size: 54 1425 50 275 18 213 967 889 Cuts at: 3899 5528 8 Size: 1629 43 Fragments arranged by size: 1629 1425 967 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3493 1353 Size: 2140 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4150 4524 4670 387 Size: 1064 138 2561 374 146 1280 Fragments arranged by size: 2561 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2367 2434 2765 3626 4223 5010 5278 Size: 393 735 67 331 861 597 787 268 Cuts at: 5278 1239 Size: 1524 Fragments arranged by size: 1524 861 787 735 597 393 331 268 67 EcoNI CCTnn'n_nnAGG Cuts at: 2215 2215 Size: 5563 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FspI TGC'GCA Cuts at: 4069 4069 Size: 5563 HaeII r_GCGC'y Cuts at: 1678 2381 4710 1678 Size: 703 2329 2531 Fragments arranged by size: 2531 2329 703 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2406 2438 2466 2498 4204 Size: 32 135 32 834 32 28 32 1706 Cuts at: 4204 4236 4278 4310 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1706 834 135 42 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KasI G'GCGC_C Cuts at: 2377 2377 Size: 5563 KpnI G_GTAC'C Cuts at: 2888 2888 Size: 5563 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2657 4677 4861 1305 Size: 1352 2020 184 2007 Fragments arranged by size: 2020 2007 1352 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3741 Fragments arranged by size: 3741 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3521 3880 4039 518 Size: 648 2355 359 159 2042 Fragments arranged by size: 2355 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2979 3656 4804 5049 1606 Size: 458 915 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 458 245 NaeI GCC'GGC Cuts at: 2376 2376 Size: 5563 NarI GG'CG_CC Cuts at: 2378 2378 Size: 5563 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5011 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NgoMIV G'CCGG_C Cuts at: 2374 2374 Size: 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NsiI A_TGCA'T Cuts at: 2784 2784 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2786 2921 4466 1132 Size: 97 1557 135 1545 2229 Fragments arranged by size: 2229 1557 1545 135 97 PasI CC'CwG_GG Cuts at: 1348 2758 1348 Size: 1410 4153 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2315 2764 1108 Size: 902 305 449 3907 Fragments arranged by size: 3907 902 449 305 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5145 5177 3632 Size: 32 1481 32 4018 Fragments arranged by size: 4018 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2117 1613 Size: 504 5059 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PstI C_TGCA'G Cuts at: 830 2915 830 Size: 2085 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 SacII CC_GC'GG Cuts at: 2065 2065 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2911 4046 4727 4918 826 Size: 219 1866 1135 681 191 1471 Fragments arranged by size: 1866 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2379 2379 Size: 5563 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2890 1872 Size: 1018 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3667 4568 4830 820 Size: 197 926 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 2204 1517 Size: 687 4876 StyI C'CwwG_G Cuts at: 513 1065 2040 3002 513 Size: 552 975 962 3074 Fragments arranged by size: 3074 975 962 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2679 3590 3607 3760 3945 4390 325 Size: 1255 1099 911 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1099 911 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3809 5521 96 Size: 274 80 33 51 1443 1832 1712 138 Fragments arranged by size: 1832 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 2759 2791 5523 5555 2686 Size: 32 41 32 2732 32 2694 Fragments arranged by size: 2732 2694 41 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2420 1316 Size: 1104 4459 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5563 XmaI C'CCGG_G Cuts at: 1870 2888 1870 Size: 1018 4545 XmnI GAAnn'nnTTC Cuts at: 3692 3692 Size: 5563 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI