################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 476 CGCG CG^CG AcsI 77 AAATTC [GA]^AATT[CT] Alw44I 346 GTGCAC G^TGCAC AlwNI 467 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 346 GTGCAC G^TGCAC ApoI 77 AAATTC [GA]^AATT[CT] Asp700I 73 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 346 GTGCAC G[AT]GC[AT]^C AvaII 261 GGACC G^G[AT]CC BbvI 466 GCAGC GCAGC(8/12) BcgI 87 GCAGATTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 346 GTGCAC G[AGT]GC[ACT]^C BsiHKAI 346 GTGCAC G[AT]GC[AT]^C BsmAI 100 GAGAC GTCTC(1/5) BsmFI 567 GTCCC GGGAC(10/14) Bsp1286I 346 GTGCAC G[AGT]GC[ACT]^C BspMI 506 ACCTGC ACCTGC(4/8) BsrFI 194 ACCGGC [GA]^CCGG[CT] BsrI 570 CCAGT ACTGG(1/-1) BstAPI 466 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 301 TTCGAA TT^CGAA BstUI 476 CGCG CG^CG Cac8I 477 GCGGGC GC[ACGT]^[ACGT]GC CfoI 475 GCGC GCG^C Cfr10I 194 ACCGGC [GA]^CCGG[CT] Cfr13I 261 GGACC G^G[ACGT]CC EcoT14I 30 CCATGG C^C[AT][AT]GG Fnu4HI 466 GCAGC GC^[ACGT]GC HaeIII 457 GGCC GG^CC HapII 195 CCGG C^CGG HhaI 475 GCGC GCG^C HinP1I 475 GCGC G^CGC HpaII 195 CCGG C^CGG HphI 369 GGTGA GGTGA(8/7) ItaI 466 GCAGC GC^[ACGT]GC MaeIII 140 GTAAC ^GT[ACGT]AC MspI 195 CCGG C^CGG MvnI 476 CGCG CG^CG NcoI 30 CCATGG C^CATGG NdeI 334 CATATG CA^TATG NspV 301 TTCGAA TT^CGAA PflMI 360 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau96I 261 GGACC G^G[ACGT]CC SfuI 301 TTCGAA TT^CGAA SmlI 118 CTCAAG C^T[CT][GA]AG StyI 30 CCATGG C^C[AT][AT]GG TseI 466 GCAGC G^C[AT]GC Van91I 360 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 73 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BfaI 46 CTAG C^TAG 112 CTAG BpmI 358 CTCCAG CTGGAG(16/14) 439 CTGGAG BssKI 270 CCAGG ^CC[ACGT]GG 360 CCAGG BstNI 270 CCAGG CC^[AT]GG 360 CCAGG DdeI 237 CTCAG C^T[ACGT]AG 416 CTCAG DsaI 30 CCATGG C^C[GA][CT]GG 40 CCACGG EcoNI 264 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 357 CCTCCAGGAGG EcoRII 270 CCAGG ^CC[AT]GG 360 CCAGG FauI 123 GCGGG CCCGC(4/6) 477 GCGGG MaeI 46 CTAG C^TAG 112 CTAG MboII 73 GAAGA GAAGA(8/7) 329 TCTTC MvaI 270 CCAGG CC^[AT]GG 360 CCAGG MwoI 466 GCAGCCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 481 GCTGTGTCTGC NlaIII 31 CATG CATG^ 399 CATG ScrFI 270 CCAGG CC^[ACGT]GG 360 CCAGG TaqI 96 TCGA T^CGA 302 TCGA Tsp509I 78 AATT ^AATT 317 AATT TthHB8I 96 TCGA T^CGA 302 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI BsaJI BsiYI BslI BstF5I DpnII FokI HinfI MboI MnlI NdeII Sau3AI TfiI