################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 859 AAATTC [GA]^AATT[CT] AfaI 309 GTAC GT^AC ApoI 859 AAATTC [GA]^AATT[CT] Asp700I 885 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 844 CTCGAG C^[CT]CG[GA]G BalI 501 TGGCCA TGG^CCA BbeI 184 GGCGCC GGCGC^C BmyI 258 GGGCAC G[AGT]GC[ACT]^C BpmI 487 CTGGAG CTGGAG(16/14) BsoBI 844 CTCGAG C^[CT]CG[GA]G Bsp1286I 258 GGGCAC G[AGT]GC[ACT]^C BspMI 877 ACCTGC ACCTGC(4/8) BsrFI 130 ACCGGC [GA]^CCGG[CT] BssHII 20 GCGCGC G^CGCGC Cfr10I 130 ACCGGC [GA]^CCGG[CT] EarI 387 GAAGAG CTCTTC(1/4) EcoT22I 799 ATGCAT ATGCA^T KasI 184 GGCGCC G^GCGCC Ksp632I 387 GAAGAG CTCTTC(1/4) MaeII 119 ACGT A^CGT MaeIII 344 GTGAC ^GT[ACGT]AC MboII 387 GAAGA GAAGA(8/7) MluNI 501 TGGCCA TGG^CCA MscI 501 TGGCCA TGG^CCA NarI 184 GGCGCC GG^CGCC NsiI 799 ATGCAT ATGCA^T NspI 801 GCATGC [GA]CATG^[CT] PaeR7I 844 CTCGAG C^TCGAG PpuMI 101 GGGACCT [GA]G^G[AT]CC[CT] PstI 493 CTGCAG CTGCA^G RsaI 309 GTAC GT^AC SapI 387 GAAGAGC GCTCTTC(1/4) SfoI 184 GGCGCC GGC^GCC SgrAI 129 CACCGGCG C[GA]^CCGG[CT]G SphI 801 GCATGC GCATG^C StuI 467 AGGCCT AGG^CCT Tsp45I 344 GTGAC ^GT[GC]AC Tsp509I 860 AATT ^AATT XhoI 844 CTCGAG C^TCGAG XmnI 885 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 152 GGCGTC G[GA]^CG[CT]C 184 GGCGCC AlwI 246 GATCC GGATC(4/5) 852 GATCC BanI 184 GGCGCC G^G[CT][GA]CC 550 GGTGCC BsaHI 152 GGCGTC G[GA]^CG[CT]C 184 GGCGCC BsmAI 232 GAGAC GTCTC(1/5) 730 GAGAC BsmBI 232 GAGACG CGTCTC(1/5) 730 GAGACG BsmFI 101 GGGAC GGGAC(10/14) 426 GTCCC BsrI 78 ACTGG ACTGG(1/-1) 450 CCAGT BstAPI 260 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 532 GCACTGTCTGC BstYI 245 AGATCC [GA]^GATC[CT] 851 AGATCC DraII 101 GGGACCT [GA]G^G[ACGT]CC[CT] 773 AGGCCCT DraIII 261 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 505 CACATCGTG EaeI 317 CGGCCA [CT]^GGCC[GA] 501 TGGCCA EcoO109I 101 GGGACCT [GA]G^G[ACGT]CC[CT] 773 AGGCCCT HaeII 184 GGCGCC [GA]GCGC^[CT] 637 AGCGCC HgaI 153 GCGTC GACGC(5/10) 577 GCGTC Hin1I 152 GGCGTC G[GA]^CG[CT]C 184 GGCGCC HinfI 632 GAGTC G^A[ACGT]TC 692 GAGTC MflI 245 AGATCC [GA]^GATC[CT] 851 AGATCC NcoI 381 CCATGG C^CATGG 790 CCATGG PleI 632 GAGTC GAGTC(4/5) 692 GAGTC SfaNI 412 GATGC GCATC(5/9) 146 GCATC SfcI 40 CTACAG C^T[GA][CT]AG 493 CTGCAG SmlI 421 CTCAAG C^T[CT][GA]AG 844 CTCGAG TaqI 557 TCGA T^CGA 845 TCGA TthHB8I 557 TCGA T^CGA 845 TCGA XhoII 245 AGATCC [GA]^GATC[CT] 851 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BsaJI BseRI BsgI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI TspRI