(Circular) (Six-base) MAPSORT of: pFN21AE0702.seq Check: 5174 from: 1 to: 5923 pFN21AE0702 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2812 2812 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2091 3733 278 Size: 53 83 186 936 555 1642 2468 Fragments arranged by size: 2468 1642 936 555 186 83 53 AccI GT'mk_AC Cuts at: 1937 2646 3266 1937 Size: 709 620 4594 Fragments arranged by size: 4594 709 620 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2486 3186 3986 5370 5638 1632 Size: 854 700 800 1384 268 1917 Fragments arranged by size: 1917 1384 854 800 700 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 2722 3666 5238 2722 Size: 944 1572 3407 Fragments arranged by size: 3407 1572 944 ApaI G_GGCC'C Cuts at: 3164 3164 Size: 5923 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2471 2768 3248 1870 Size: 73 528 297 480 4545 Fragments arranged by size: 4545 528 480 297 73 BamHI G'GATC_C Cuts at: 2827 3253 3620 2827 Size: 426 367 5130 Fragments arranged by size: 5130 426 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2706 3045 3244 4699 618 Size: 325 217 627 919 339 199 1455 1842 Fragments arranged by size: 1842 1455 919 627 339 325 217 199 BanII G_rGCy'C Cuts at: 1391 2111 3164 3242 1391 Size: 720 1053 78 4072 Fragments arranged by size: 4072 1053 720 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 4559 Fragments arranged by size: 4559 971 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2626 2660 3044 3078 4114 4148 1903 Size: 34 689 34 384 34 1036 34 3678 Fragments arranged by size: 3678 1036 689 384 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 2736 1548 Size: 1188 4735 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3142 4534 136 Size: 107 122 71 631 605 1470 1392 1525 Fragments arranged by size: 1525 1470 1392 631 605 122 107 71 BglII A'GATC_T Cuts at: 2461 5918 2461 Size: 3457 2466 BlpI GC'TnA_GC Cuts at: 2181 2850 3340 2181 Size: 669 490 4764 Fragments arranged by size: 4764 669 490 Bme1580I G_kGCm'C Cuts at: 1790 3084 3164 3984 5140 1790 Size: 1294 80 820 1156 2573 Fragments arranged by size: 2573 1294 1156 820 80 BmgBI CAC'GTC Cuts at: 1318 2017 1318 Size: 699 5224 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 3094 3654 Size: 416 53 83 399 24 636 1041 560 Cuts at: 3654 4612 442 Size: 958 1753 Fragments arranged by size: 1753 1041 958 636 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3196 3196 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2794 4583 1239 Size: 986 569 1789 2579 Fragments arranged by size: 2579 1789 986 569 Bpu10I CC'TnA_GC Cuts at: 2196 2972 2196 Size: 776 5147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2415 4586 882 Size: 617 916 2171 2219 Fragments arranged by size: 2219 2171 916 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 3730 4112 4747 Size: 53 83 186 936 555 1642 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1642 1451 936 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 2792 2822 1178 Size: 30 165 30 1332 30 27 30 4279 Fragments arranged by size: 4279 1332 165 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 2222 3185 1186 Size: 929 107 963 3924 Fragments arranged by size: 3924 963 929 107 BseYI C'CCAG_C Cuts at: 3217 5126 5622 3217 Size: 1909 496 3518 Fragments arranged by size: 3518 1909 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2111 3984 4069 5140 2111 Size: 1873 85 1071 2894 Fragments arranged by size: 2894 1873 1071 85 BsmI GAATG_Cn' Cuts at: 3010 3434 3527 3010 Size: 424 93 5406 Fragments arranged by size: 5406 424 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 3084 3164 3242 3984 4069 5140 Size: 399 321 973 80 78 742 85 1071 Cuts at: 5140 1391 Size: 2174 Fragments arranged by size: 2174 1071 973 742 399 321 85 80 78 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2812 3278 844 Size: 1968 466 3489 Fragments arranged by size: 3489 1968 466 BsrBI CCG'CTC Cuts at: 1394 1823 2185 3810 1394 Size: 429 362 1625 3507 Fragments arranged by size: 3507 1625 429 362 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2258 2709 3132 4567 4754 1700 Size: 110 448 451 423 1435 187 2869 Fragments arranged by size: 2869 1435 451 448 423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 2147 3983 4995 2147 Size: 1836 1012 3075 Fragments arranged by size: 3075 1836 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2703 2011 Size: 692 5231 BstYI r'GATC_y Cuts at: 1972 2461 2502 2595 2718 2827 3253 3620 4005 Size: 489 41 93 123 109 426 367 385 Cuts at: 4005 4022 5474 5560 5572 5918 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 489 426 385 367 346 123 109 93 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2082 2832 2082 Size: 750 5173 BtgI C'CryG_G Cuts at: 513 1065 2610 513 Size: 552 1545 3826 Fragments arranged by size: 3826 1545 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2943 3439 4233 4253 2430 Size: 513 496 794 20 4100 Fragments arranged by size: 4100 794 513 496 20 ClaI AT'CG_AT Cuts at: 1554 2286 1554 Size: 732 5191 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2260 2990 Size: 54 1425 50 275 18 313 117 730 Cuts at: 2990 3370 4259 5888 8 Size: 380 889 1629 43 Fragments arranged by size: 1629 1425 889 730 380 313 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3162 3853 1353 Size: 1809 691 3423 Fragments arranged by size: 3423 1809 691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 4510 4884 5030 387 Size: 1064 138 519 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 519 374 146 138 EcoICRI GAG'CTC Cuts at: 2109 2109 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2486 2794 3186 3986 4583 5370 Size: 393 593 261 308 392 800 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 800 787 597 593 393 392 308 268 261 EcoNI CCTnn'n_nnAGG Cuts at: 2249 2249 Size: 5923 EcoO109I rG'GnC_Cy Cuts at: 2571 2857 3019 3161 2571 Size: 286 162 142 5333 Fragments arranged by size: 5333 286 162 142 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2591 5070 1678 Size: 913 2479 2531 Fragments arranged by size: 2531 2479 913 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2792 2824 4564 4596 4638 Size: 32 135 32 1220 32 1740 32 42 Cuts at: 4638 4670 1373 Size: 32 2626 Fragments arranged by size: 2626 1740 1220 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3267 3513 669 Size: 988 281 709 620 246 3079 Fragments arranged by size: 3079 988 709 620 281 246 HindIII A'AGCT_T Cuts at: 748 2552 748 Size: 1804 4119 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2530 5037 5221 1305 Size: 1225 2507 184 2007 Fragments arranged by size: 2507 2007 1225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 10 Size: 54 1425 343 313 3788 Fragments arranged by size: 3788 1425 343 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 3881 4240 4399 518 Size: 648 1572 1143 359 159 2042 Fragments arranged by size: 2042 1572 1143 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2180 2203 2849 2923 3339 4016 5164 5409 Size: 574 23 646 74 416 677 1148 245 Cuts at: 5409 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 646 574 416 245 74 23 NaeI GCC'GGC Cuts at: 2260 2260 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 2610 513 Size: 552 1545 3826 Fragments arranged by size: 3826 1545 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2258 2258 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 2576 3140 1348 Size: 1228 564 4131 Fragments arranged by size: 4131 1228 564 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2633 1108 Size: 1525 4398 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2571 3019 2571 Size: 448 5475 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 3160 3160 Size: 5923 PstI C_TGCA'G Cuts at: 830 2823 3275 830 Size: 1993 452 3478 Fragments arranged by size: 3478 1993 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2180 2203 2849 2180 Size: 23 646 5254 Fragments arranged by size: 5254 646 23 SacI G_AGCT'C Cuts at: 2111 2111 Size: 5923 SalI G'TCGA_C Cuts at: 1936 2645 3265 1936 Size: 709 620 4594 Fragments arranged by size: 4594 709 620 SanDI GG'GwC_CC Cuts at: 2571 3019 2571 Size: 448 5475 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 2393 2393 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 2819 2861 3271 4406 5087 5278 826 Size: 219 1774 42 410 1135 681 191 1471 Fragments arranged by size: 1774 1471 1135 681 410 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 3142 3142 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 2709 1700 Size: 1009 4914 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 2479 3847 5 Size: 47 2427 1368 2081 Fragments arranged by size: 2427 2081 1368 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2610 3362 513 Size: 552 1545 752 3074 Fragments arranged by size: 3074 1545 752 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 2993 3950 3967 4120 4305 4750 Size: 1255 1060 353 957 17 153 185 445 Cuts at: 4750 325 Size: 1498 Fragments arranged by size: 1498 1255 1060 957 445 353 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2540 2630 2540 Size: 90 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2089 3731 276 Size: 53 83 186 936 555 1642 2468 Fragments arranged by size: 2468 1642 936 555 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI