(Circular) (Six-base) MAPSORT of: pFN21AE1329.seq Check: 4075 from: 1 to: 6496 pFN21AE1329 6496 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3839 1937 Size: 694 1208 4594 Fragments arranged by size: 4594 1208 694 Acc65I G'GTAC_C Cuts at: 2340 3817 2340 Size: 1477 5019 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4559 5943 6211 1632 Size: 2927 1384 268 1917 Fragments arranged by size: 2927 1917 1384 268 AfeI AGC'GCT Cuts at: 3494 3494 Size: 6496 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 2352 5395 6372 1203 Size: 1149 3043 977 1327 Fragments arranged by size: 3043 1327 1149 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 2660 1166 Size: 1494 5002 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AlwNI CAG_nnn'CTG Cuts at: 2797 3253 4239 5811 2797 Size: 456 986 1572 3482 Fragments arranged by size: 3482 1572 986 456 ApaI G_GGCC'C Cuts at: 3138 3354 3138 Size: 216 6280 ApaLI G'TGCA_C Cuts at: 3362 4553 5709 3362 Size: 1191 1156 4149 Fragments arranged by size: 4149 1191 1156 ApoI r'AATT_y Cuts at: 1493 3805 4031 4183 6292 1493 Size: 2312 226 152 2109 1697 Fragments arranged by size: 2312 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2900 3063 3107 3496 3821 1870 Size: 73 957 163 44 389 325 4545 Fragments arranged by size: 4545 957 389 325 163 73 44 BamHI G'GATC_C Cuts at: 2169 3826 4193 2169 Size: 1657 367 4472 Fragments arranged by size: 4472 1657 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2340 2621 3392 3817 Size: 325 217 627 495 58 281 771 425 Cuts at: 3817 5272 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 771 627 495 425 325 281 217 58 BanII G_rGCy'C Cuts at: 1391 2400 3138 3354 3815 1391 Size: 1009 738 216 461 4072 Fragments arranged by size: 4072 1009 738 461 216 BbeI G_GCGC'C Cuts at: 2625 2625 Size: 6496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2603 3473 928 Size: 971 704 870 3951 Fragments arranged by size: 3951 971 870 704 BbvCI CC'TCA_GC Cuts at: 2426 3299 2426 Size: 873 5623 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4687 4721 1903 Size: 34 50 34 2666 34 3678 Fragments arranged by size: 3678 2666 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2716 4388 5598 2716 Size: 1672 1210 3614 Fragments arranged by size: 3614 1672 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3390 5107 136 Size: 107 122 71 631 605 1718 1717 1525 Fragments arranged by size: 1718 1717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3234 6491 3234 Size: 3257 3239 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 3138 3354 3366 4557 5713 1790 Size: 1348 216 12 1191 1156 2573 Fragments arranged by size: 2573 1348 1191 1156 216 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2097 3273 3544 Size: 416 53 83 399 24 680 1176 271 Cuts at: 3544 4227 5185 442 Size: 683 958 1753 Fragments arranged by size: 1753 1176 958 683 680 416 399 271 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2632 3445 2632 Size: 813 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2457 3014 3599 5156 1239 Size: 1218 557 585 1557 2579 Fragments arranged by size: 2579 1557 1218 585 557 Bpu10I CC'TnA_GC Cuts at: 2426 2495 3254 3299 2426 Size: 69 759 45 5623 Fragments arranged by size: 5623 759 69 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 2282 3770 4621 5486 5784 2048 Size: 234 1488 851 865 298 2760 Fragments arranged by size: 2760 1488 865 851 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2025 2048 2549 3205 3213 5159 882 Size: 617 526 23 501 656 8 1946 2219 Fragments arranged by size: 2219 1946 656 617 526 501 23 8 BsaAI yAC'GTr Cuts at: 493 1204 3538 4287 493 Size: 711 2334 749 2702 Fragments arranged by size: 2702 2334 749 711 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 4303 4685 5320 Size: 53 83 186 936 1089 1681 382 635 Cuts at: 5320 275 Size: 1451 Fragments arranged by size: 1681 1451 1089 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 3506 3536 3599 Size: 30 165 30 1027 30 1046 30 63 Cuts at: 3599 3629 3683 3713 1178 Size: 30 54 30 3961 Fragments arranged by size: 3961 1046 1027 165 63 54 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 2762 3746 1186 Size: 1438 138 984 3936 Fragments arranged by size: 3936 1438 984 138 BseYI C'CCAG_C Cuts at: 3637 5699 6195 3637 Size: 2062 496 3938 Fragments arranged by size: 3938 2062 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3527 3695 1163 Size: 573 1791 168 3964 Fragments arranged by size: 3964 1791 573 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 3946 4707 5735 1540 Size: 275 186 1384 561 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 561 275 186 BsiHKAI G_wGCw'C Cuts at: 2460 3366 4557 4642 5713 2460 Size: 906 1191 85 1071 3243 Fragments arranged by size: 3243 1191 1071 906 85 BsmI GAATG_Cn' Cuts at: 2671 3655 4007 4100 2671 Size: 984 352 93 5067 Fragments arranged by size: 5067 984 352 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2366 Size: 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2460 3138 3354 3366 3815 4557 Size: 399 610 60 678 216 12 449 742 Cuts at: 4557 4642 5713 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 678 610 449 399 216 85 60 12 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 3054 4385 6115 3054 Size: 1331 1730 3435 Fragments arranged by size: 3435 1730 1331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2643 3717 3851 844 Size: 1799 1074 134 3489 Fragments arranged by size: 3489 1799 1074 134 BsrBI CCG'CTC Cuts at: 1394 1823 2494 4383 1394 Size: 429 671 1889 3507 Fragments arranged by size: 3507 1889 671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3374 5140 5327 1700 Size: 110 1564 1766 187 2869 Fragments arranged by size: 2869 1766 1564 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 2656 4556 5568 2656 Size: 1900 1012 3584 Fragments arranged by size: 3584 1900 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6496 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 2155 2500 1184 Size: 971 345 5180 Fragments arranged by size: 5180 971 345 BstXI CCAn_nnnn'nTGG Cuts at: 2357 3442 3478 2357 Size: 1085 36 5375 Fragments arranged by size: 5375 1085 36 BstYI r'GATC_y Cuts at: 1972 2169 3234 3826 4193 4578 4595 6047 6133 Size: 197 1065 592 367 385 17 1452 86 Cuts at: 6133 6145 6491 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1065 592 385 367 346 197 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2617 2617 Size: 6496 BtgI C'CryG_G Cuts at: 513 1065 2014 2080 2344 3000 3268 513 Size: 552 949 66 264 656 268 3741 Fragments arranged by size: 3741 949 656 552 268 264 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2959 3529 487 Size: 683 56 1733 570 3454 Fragments arranged by size: 3454 1733 683 570 56 BtsI GCAGTG_nn' Cuts at: 3364 4012 4806 4826 3364 Size: 648 794 20 5034 Fragments arranged by size: 5034 794 648 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3272 5503 809 Size: 507 1956 2231 1802 Fragments arranged by size: 2231 1956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 2810 3382 Size: 54 1425 50 275 18 517 463 572 Cuts at: 3382 3540 3943 4832 6461 8 Size: 158 403 889 1629 43 Fragments arranged by size: 1629 1425 889 572 517 463 403 275 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 3943 1537 Size: 275 1570 561 4090 Fragments arranged by size: 4090 1570 561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3764 4426 1353 Size: 2411 662 3423 Fragments arranged by size: 3423 2411 662 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2457 3014 3599 4559 5156 5943 6211 Size: 393 825 557 585 960 597 787 268 Cuts at: 6211 1239 Size: 1524 Fragments arranged by size: 1524 960 825 787 597 585 557 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2063 3345 2063 Size: 1282 5214 EcoO109I rG'GnC_Cy Cuts at: 2948 3135 3274 3350 3439 3583 2948 Size: 187 139 76 89 144 5861 Fragments arranged by size: 5861 187 144 139 89 76 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2625 3375 3496 5643 1678 Size: 947 750 121 2147 2531 Fragments arranged by size: 2531 2147 947 750 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5137 5169 5211 5243 1373 Size: 32 135 32 3565 32 42 32 2626 Fragments arranged by size: 3565 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3840 4086 669 Size: 988 281 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KasI G'GCGC_C Cuts at: 2621 2621 Size: 6496 KpnI G_GTAC'C Cuts at: 2344 3821 2344 Size: 1477 5019 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 2931 3591 5610 5794 1305 Size: 1368 258 660 2019 184 2007 Fragments arranged by size: 2019 2007 1368 660 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 3542 10 Size: 54 1425 343 517 1193 2964 Fragments arranged by size: 2964 1425 1193 517 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 3053 3359 3410 3416 3476 3536 Size: 648 1494 393 306 51 6 60 60 Cuts at: 3536 4454 4813 4972 518 Size: 918 359 159 2042 Fragments arranged by size: 2042 1494 918 648 393 359 306 159 60 60 51 6 MspA1I CmG'CkG Cuts at: 1606 2308 2809 2941 3208 3325 3751 3912 4589 Size: 702 501 132 267 117 426 161 677 Cuts at: 4589 5737 5982 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 702 677 501 426 267 245 161 132 117 NaeI GCC'GGC Cuts at: 3376 3376 Size: 6496 NarI GG'CG_CC Cuts at: 2622 2622 Size: 6496 NcoI C'CATG_G Cuts at: 513 1065 2344 3268 513 Size: 552 1279 924 3741 Fragments arranged by size: 3741 1279 924 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NgoMIV G'CCGG_C Cuts at: 3374 3374 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 2356 3121 3415 3854 5399 1132 Size: 97 1127 765 294 439 1545 2229 Fragments arranged by size: 2229 1545 1127 765 439 294 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 2352 5395 2352 Size: 3043 3453 PflMI CCAn_nnn'nTGG Cuts at: 1108 2797 1108 Size: 1689 4807 PfoI T'CCnGG_A Cuts at: 3306 3306 Size: 6496 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3763 3795 4565 4597 6078 6110 3763 Size: 32 770 32 1481 32 4149 Fragments arranged by size: 4149 1481 770 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3274 3583 3274 Size: 309 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 4066 4066 Size: 6496 PspOMI G'GGCC_C Cuts at: 3134 3350 3134 Size: 216 6280 PstI C_TGCA'G Cuts at: 830 2271 2385 2880 3371 3591 3848 830 Size: 1441 114 495 491 220 257 3478 Fragments arranged by size: 3478 1441 495 491 257 220 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2941 2941 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SanDI GG'GwC_CC Cuts at: 3274 3274 Size: 6496 SapI GCTCTTCn'nnn_ Cuts at: 3764 3764 Size: 6496 SbfI CC_TGCA'GG Cuts at: 2271 2880 3848 2271 Size: 609 968 4919 Fragments arranged by size: 4919 968 609 ScaI AGT'ACT Cuts at: 2243 4744 6456 2243 Size: 2501 1712 2283 Fragments arranged by size: 2501 2283 1712 SexAI A'CCwGG_T Cuts at: 2577 2577 Size: 6496 SfcI C'TryA_G Cuts at: 826 1045 2267 2381 2876 3367 3587 3844 4979 Size: 219 1222 114 495 491 220 257 1135 Cuts at: 4979 5660 5851 826 Size: 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 495 491 257 220 219 191 114 SfoI GGC'GCC Cuts at: 2623 2623 Size: 6496 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 2902 3823 1872 Size: 1030 921 4545 Fragments arranged by size: 4545 1030 921 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 2297 3785 4600 5501 5763 Size: 197 926 120 234 1488 815 901 262 Cuts at: 5763 820 Size: 1553 Fragments arranged by size: 1553 1488 926 901 815 262 234 197 120 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 2327 152 Size: 2175 4321 SphI G_CATG'C Cuts at: 1132 3121 3854 1132 Size: 1989 733 3774 Fragments arranged by size: 3774 1989 733 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 2615 3665 1517 Size: 1098 1050 4348 Fragments arranged by size: 4348 1098 1050 StyI C'CwwG_G Cuts at: 513 1065 2344 2388 2472 2730 3268 3435 3660 Size: 552 1279 44 84 258 538 167 225 Cuts at: 3660 3935 513 Size: 275 3074 Fragments arranged by size: 3074 1279 552 538 275 258 225 167 84 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2007 2519 2534 4523 4540 4693 4878 Size: 1255 427 512 15 1989 17 153 185 Cuts at: 4878 5323 325 Size: 445 1498 Fragments arranged by size: 1989 1498 1255 512 445 427 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 3522 4742 Size: 274 80 33 51 1443 264 1281 1220 Cuts at: 4742 6454 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1281 1220 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 6456 6488 2430 Size: 32 3994 32 2438 Fragments arranged by size: 3994 2438 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2474 3067 3439 2474 Size: 593 372 5531 Fragments arranged by size: 5531 593 372 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 2900 3821 1870 Size: 1030 921 4545 Fragments arranged by size: 4545 1030 921 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI