(Linear) (Six-base) MAPSORT of: KIEE1329.seq Check: 7019 from: 1 to: 1791 KIEE1329.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AccI GT'mk_AC Cuts at: 0 626 1791 Size: 626 1165 Acc65I G'GTAC_C Cuts at: 0 335 1791 Size: 335 1456 AfeI AGC'GCT Cuts at: 0 1489 1791 Size: 1489 302 AflIII A'CryG_T Cuts at: 0 347 1791 Size: 347 1444 AleI CACnn'nnGTG Cuts at: 0 655 1791 Size: 655 1136 AlwNI CAG_nnn'CTG Cuts at: 0 792 1248 1791 Size: 792 456 543 Fragments arranged by size: 792 543 456 ApaI G_GGCC'C Cuts at: 0 1133 1349 1791 Size: 1133 216 442 Fragments arranged by size: 1133 442 216 ApaLI G'TGCA_C Cuts at: 0 1357 1791 Size: 1357 434 AvaI C'yCGr_G Cuts at: 0 895 1058 1102 1491 1791 Size: 895 163 44 389 300 Fragments arranged by size: 895 389 300 163 44 BamHI G'GATC_C Cuts at: 0 164 1791 Size: 164 1627 BanI G'GyrC_C Cuts at: 0 277 335 616 1387 1791 Size: 277 58 281 771 404 Fragments arranged by size: 771 404 281 277 58 BanII G_rGCy'C Cuts at: 0 395 1133 1349 1791 Size: 395 738 216 442 Fragments arranged by size: 738 442 395 216 BbeI G_GCGC'C Cuts at: 0 620 1791 Size: 620 1171 BbsI GAAGACnn'nnnn_ Cuts at: 0 598 1468 1791 Size: 598 870 323 Fragments arranged by size: 870 598 323 BbvCI CC'TCA_GC Cuts at: 0 421 1294 1791 Size: 421 873 497 Fragments arranged by size: 873 497 421 BciVI GTATCCnnnnn_n' Cuts at: 0 711 1791 Size: 711 1080 BglI GCCn_nnn'nGGC Cuts at: 0 1385 1791 Size: 1385 406 BglII A'GATC_T Cuts at: 0 1229 1791 Size: 1229 562 Bme1580I G_kGCm'C Cuts at: 0 1133 1349 1361 1791 Size: 1133 216 12 430 Fragments arranged by size: 1133 430 216 12 BmrI ACTGGGnnnn_n' Cuts at: 0 92 1268 1539 1791 Size: 92 1176 271 252 Fragments arranged by size: 1176 271 252 92 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 627 1440 1791 Size: 627 813 351 Fragments arranged by size: 813 627 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1009 1594 1791 Size: 452 557 585 197 Fragments arranged by size: 585 557 452 197 Bpu10I CC'TnA_GC Cuts at: 0 421 490 1249 1294 1791 Size: 421 69 759 45 497 Fragments arranged by size: 759 497 421 69 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 277 1765 1791 Size: 43 234 1488 26 Fragments arranged by size: 1488 234 43 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 20 43 544 1200 1208 1791 Size: 20 23 501 656 8 583 Fragments arranged by size: 656 583 501 23 20 8 BsaAI yAC'GTr Cuts at: 0 1533 1791 Size: 1533 258 BsaHI Gr'CG_yC Cuts at: 0 617 1791 Size: 617 1174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 425 455 1501 1531 1594 1624 1678 1708 Size: 425 30 1046 30 63 30 54 30 Cuts at: 1708 1791 Size: 83 Fragments arranged by size: 1046 425 83 63 54 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 619 757 1741 1791 Size: 619 138 984 50 Fragments arranged by size: 984 619 138 50 BseYI C'CCAG_C Cuts at: 0 1632 1791 Size: 1632 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1522 1690 1791 Size: 1522 168 101 Fragments arranged by size: 1522 168 101 BsiEI CG_ry'CG Cuts at: 0 1380 1791 Size: 1380 411 BsiHKAI G_wGCw'C Cuts at: 0 455 1361 1791 Size: 455 906 430 Fragments arranged by size: 906 455 430 BsmI GAATG_Cn' Cuts at: 0 666 1650 1791 Size: 666 984 141 Fragments arranged by size: 984 666 141 BsmBI CGTCTCn'nnnn_ Cuts at: 0 361 1791 Size: 361 1430 Bsp1286I G_dGCh'C Cuts at: 0 395 455 1133 1349 1361 1791 Size: 395 60 678 216 12 430 Fragments arranged by size: 678 430 395 216 60 12 BspHI T'CATG_A Cuts at: 0 1049 1791 Size: 1049 742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 638 1712 1791 Size: 638 1074 79 Fragments arranged by size: 1074 638 79 BsrBI CCG'CTC Cuts at: 0 489 1791 Size: 489 1302 BsrFI r'CCGG_y Cuts at: 0 1369 1791 Size: 1369 422 BssSI C'ACGA_G Cuts at: 0 651 1791 Size: 651 1140 BstEII G'GTnAC_C Cuts at: 0 150 495 1791 Size: 150 345 1296 Fragments arranged by size: 1296 345 150 BstXI CCAn_nnnn'nTGG Cuts at: 0 352 1437 1473 1791 Size: 352 1085 36 318 Fragments arranged by size: 1085 352 318 36 BstYI r'GATC_y Cuts at: 0 164 1229 1791 Size: 164 1065 562 Fragments arranged by size: 1065 562 164 Bsu36I CC'TnA_GG Cuts at: 0 612 1791 Size: 612 1179 BtgI C'CryG_G Cuts at: 0 9 75 339 995 1263 1791 Size: 9 66 264 656 268 528 Fragments arranged by size: 656 528 268 264 66 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 954 1524 1791 Size: 954 570 267 Fragments arranged by size: 954 570 267 BtsI GCAGTG_nn' Cuts at: 0 1359 1791 Size: 1359 432 DrdI GACnn_nn'nnGTC Cuts at: 0 1267 1791 Size: 1267 524 EaeI y'GGCC_r Cuts at: 0 342 805 1377 1535 1791 Size: 342 463 572 158 256 Fragments arranged by size: 572 463 342 256 158 EagI C'GGCC_G Cuts at: 0 1377 1791 Size: 1377 414 EarI CTCTTCn'nnn_ Cuts at: 0 1759 1791 Size: 1759 32 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1009 1594 1791 Size: 452 557 585 197 Fragments arranged by size: 585 557 452 197 EcoNI CCTnn'n_nnAGG Cuts at: 0 58 1340 1791 Size: 58 1282 451 Fragments arranged by size: 1282 451 58 EcoO109I rG'GnC_Cy Cuts at: 0 943 1130 1269 1345 1434 1578 1791 Size: 943 187 139 76 89 144 213 Fragments arranged by size: 943 213 187 144 139 89 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 750 1791 Size: 750 1041 HaeII r_GCGC'y Cuts at: 0 620 1370 1491 1791 Size: 620 750 121 300 Fragments arranged by size: 750 620 300 121 KasI G'GCGC_C Cuts at: 0 616 1791 Size: 616 1175 KpnI G_GTAC'C Cuts at: 0 339 1791 Size: 339 1452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 668 926 1586 1791 Size: 668 258 660 205 Fragments arranged by size: 668 660 258 205 MscI TGG'CCA Cuts at: 0 344 1537 1791 Size: 344 1193 254 Fragments arranged by size: 1193 344 254 MslI CAynn'nnrTG Cuts at: 0 655 1048 1354 1405 1411 1471 1531 1791 Size: 655 393 306 51 6 60 60 260 Fragments arranged by size: 655 393 306 260 60 60 51 6 MspA1I CmG'CkG Cuts at: 0 303 804 936 1203 1320 1746 1791 Size: 303 501 132 267 117 426 45 Fragments arranged by size: 501 426 303 267 132 117 45 NaeI GCC'GGC Cuts at: 0 1371 1791 Size: 1371 420 NarI GG'CG_CC Cuts at: 0 617 1791 Size: 617 1174 NcoI C'CATG_G Cuts at: 0 339 1263 1791 Size: 339 924 528 Fragments arranged by size: 924 528 339 NgoMIV G'CCGG_C Cuts at: 0 1369 1791 Size: 1369 422 NspI r_CATG'y Cuts at: 0 351 1116 1410 1791 Size: 351 765 294 381 Fragments arranged by size: 765 381 351 294 PciI A'CATG_T Cuts at: 0 347 1791 Size: 347 1444 PflMI CCAn_nnn'nTGG Cuts at: 0 792 1791 Size: 792 999 PfoI T'CCnGG_A Cuts at: 0 1301 1791 Size: 1301 490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1758 1790 1791 Size: 1758 32 1 Fragments arranged by size: 1758 32 1 PpuMI rG'GwC_Cy Cuts at: 0 1269 1578 1791 Size: 1269 309 213 Fragments arranged by size: 1269 309 213 PspOMI G'GGCC_C Cuts at: 0 1129 1345 1791 Size: 1129 216 446 Fragments arranged by size: 1129 446 216 PstI C_TGCA'G Cuts at: 0 266 380 875 1366 1586 1791 Size: 266 114 495 491 220 205 Fragments arranged by size: 495 491 266 220 205 114 PvuII CAG'CTG Cuts at: 0 936 1791 Size: 936 855 SanDI GG'GwC_CC Cuts at: 0 1269 1791 Size: 1269 522 SapI GCTCTTCn'nnn_ Cuts at: 0 1759 1791 Size: 1759 32 SbfI CC_TGCA'GG Cuts at: 0 266 875 1791 Size: 266 609 916 Fragments arranged by size: 916 609 266 ScaI AGT'ACT Cuts at: 0 238 1791 Size: 238 1553 SexAI A'CCwGG_T Cuts at: 0 572 1791 Size: 572 1219 SfcI C'TryA_G Cuts at: 0 262 376 871 1362 1582 1791 Size: 262 114 495 491 220 209 Fragments arranged by size: 495 491 262 220 209 114 SfoI GGC'GCC Cuts at: 0 618 1791 Size: 618 1173 SmaI CCC'GGG Cuts at: 0 897 1791 Size: 897 894 SmlI C'TyrA_G Cuts at: 0 58 292 1780 1791 Size: 58 234 1488 11 Fragments arranged by size: 1488 234 58 11 SpeI A'CTAG_T Cuts at: 0 322 1791 Size: 322 1469 SphI G_CATG'C Cuts at: 0 1116 1791 Size: 1116 675 StuI AGG'CCT Cuts at: 0 610 1660 1791 Size: 610 1050 131 Fragments arranged by size: 1050 610 131 StyI C'CwwG_G Cuts at: 0 339 383 467 725 1263 1430 1655 1791 Size: 339 44 84 258 538 167 225 136 Fragments arranged by size: 538 339 258 225 167 136 84 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2 514 529 1791 Size: 2 512 15 1262 Fragments arranged by size: 1262 512 15 2 TatI w'GTAC_w Cuts at: 0 236 1517 1791 Size: 236 1281 274 Fragments arranged by size: 1281 274 236 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 425 457 1791 Size: 425 32 1334 Fragments arranged by size: 1334 425 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 469 1062 1434 1791 Size: 469 593 372 357 Fragments arranged by size: 593 469 372 357 XmaI C'CCGG_G Cuts at: 0 895 1791 Size: 895 896 Enzymes that do cut: AccI Acc65I AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiWI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI