Restriction map for
pFN21AE9659
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvrII
BamHI BbrPI BcgI BciVI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HinfI HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
TspRI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 91 CGCG CG^CG
AflII 646 CTTAAG C^TTAAG
AlwNI 496 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 59 GGGCCC GGGCC^C
BalI 149 TGGCCA TGG^CCA
BbeI 17 GGCGCC GGCGC^C
BbsI 24 GAAGAC GAAGAC(2/6)
BbvCI 605 CCTCAGC CCTCAGC(-5/-2)
BcnI 206 CCCGG CC^[GC]GG
BfrI 646 CTTAAG C^TTAAG
BpmI 634 CTGGAG CTGGAG(16/14)
BpuAI 24 GAAGAC GAAGAC(2/6)
BsaI 109 GAGACC GGTCTC(1/5)
BsmAI 109 GAGAC GTCTC(1/5)
BspHI 374 TCATGA T^CATGA
BspMI 653 ACCTGC ACCTGC(4/8)
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BsrDI 129 CATTGC GCAATG(2/0)
BsrI 553 ACTGG ACTGG(1/-1)
BstUI 91 CGCG CG^CG
BstXI 320 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 273 GACACTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 149 TGGCCA [CT]^GGCC[GA]
EarI 469 GAAGAG CTCTTC(1/4)
HgaI 27 GACGC GACGC(5/10)
KasI 17 GGCGCC G^GCGCC
Ksp632I 469 GAAGAG CTCTTC(1/4)
MaeIII 316 GTCAC ^GT[ACGT]AC
MluNI 149 TGGCCA TGG^CCA
MscI 149 TGGCCA TGG^CCA
MslI 405 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 346 CAGCTG C[AC]G^C[GT]G
MvnI 91 CGCG CG^CG
NarI 17 GGCGCC GG^CGCC
NciI 206 CCCGG CC^[GC]GG
PflMI 495 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 346 CAGCTG CAG^CTG
RcaI 374 TCATGA T^CATGA
SfoI 17 GGCGCC GGC^GCC
Tsp45I 316 GTCAC ^GT[GC]AC
Van91I 495 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 17 GGCGCC G[GA]^CG[CT]C
27 GACGCC
AlwI 267 GATCC GGATC(4/5)
363 GATCC
AvaII 3 GGACC G^G[AT]CC
87 GGACC
AviII 200 TGCGCA TGC^GCA
218 TGCGCA
BanI 17 GGCGCC G^G[CT][GA]CC
331 GGCACC
BanII 59 GGGCCC G[GA]GC[CT]^C
558 GGGCTC
BbvI 601 GCAGC GCAGC(8/12)
574 GCTGC
BfaI 106 CTAG C^TAG
613 CTAG
BsaHI 17 GGCGCC G[GA]^CG[CT]C
27 GACGCC
BstYI 266 AGATCC [GA]^GATC[CT]
362 AGATCC
DraII 58 AGGGCCC [GA]G^G[ACGT]CC[CT]
59 GGGCCCT
DsaI 326 CCATGG C^C[GA][CT]GG
449 CCATGG
EcoO109I 58 AGGGCCC [GA]G^G[ACGT]CC[CT]
59 GGGCCCT
FauI 78 GCGGG CCCGC(4/6)
237 GCGGG
FspI 200 TGCGCA TGC^GCA
218 TGCGCA
HaeII 17 GGCGCC [GA]GCGC^[CT]
209 GGCGCT
HapII 21 CCGG C^CGG
207 CCGG
Hin1I 17 GGCGCC G[GA]^CG[CT]C
27 GACGCC
HpaII 21 CCGG C^CGG
207 CCGG
HphI 454 GGTGA GGTGA(8/7)
317 TCACC
MaeI 106 CTAG C^TAG
613 CTAG
MflI 266 AGATCC [GA]^GATC[CT]
362 AGATCC
MseI 140 TTAA T^TAA
647 TTAA
MspI 21 CCGG C^CGG
207 CCGG
NcoI 326 CCATGG C^CATGG
449 CCATGG
TaqI 292 TCGA T^CGA
538 TCGA
Tru9I 140 TTAA T^TAA
647 TTAA
TseI 574 GCTGC G^C[AT]GC
601 GCAGC
TthHB8I 292 TCGA T^CGA
538 TCGA
XcmI 74 CCATGCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
284 CCAACAACTCGATGG
XhoII 266 AGATCC [GA]^GATC[CT]
362 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SmlI StyI