(Circular) (Six-base) MAPSORT of: pFN21AE9659.seq Check: 6872 from: 1 to: 5371 pFN21AE9659 5371 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3181 278 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2714 1937 Size: 777 4594 Acc65I G'GTAC_C Cuts at: 2692 2692 Size: 5371 AclI AA'CG_TT Cuts at: 1563 3498 3871 1563 Size: 1935 373 3063 Fragments arranged by size: 3063 1935 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 3434 4818 5086 1632 Size: 743 1059 1384 268 1917 Fragments arranged by size: 1917 1384 1059 743 268 AflII C'TTAA_G Cuts at: 820 1017 2651 820 Size: 197 1634 3540 Fragments arranged by size: 3540 1634 197 AflIII A'CryG_T Cuts at: 1203 4270 5247 1203 Size: 3067 977 1327 Fragments arranged by size: 3067 1327 977 AgeI A'CCGG_T Cuts at: 4202 4202 Size: 5371 AhdI GACnn_n'nnGTC Cuts at: 4100 4100 Size: 5371 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5371 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 5339 AlwNI CAG_nnn'CTG Cuts at: 2506 3114 4686 2506 Size: 608 1572 3191 Fragments arranged by size: 3191 1572 608 ApaI G_GGCC'C Cuts at: 2068 2068 Size: 5371 ApaLI G'TGCA_C Cuts at: 3428 4584 3428 Size: 1156 4215 ApoI r'AATT_y Cuts at: 1493 2680 2906 3058 5167 1493 Size: 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 226 152 AseI AT'TA_AT Cuts at: 160 1032 3925 160 Size: 872 2893 1606 Fragments arranged by size: 2893 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 AvaI C'yCGr_G Cuts at: 1870 1943 2696 1870 Size: 73 753 4545 Fragments arranged by size: 4545 753 73 BamHI G'GATC_C Cuts at: 2701 3068 2701 Size: 367 5004 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2336 2692 4147 618 Size: 325 217 627 235 314 356 1455 1842 Fragments arranged by size: 1842 1455 627 356 325 314 235 217 BanII G_rGCy'C Cuts at: 1391 2068 2567 2690 1391 Size: 677 499 123 4072 Fragments arranged by size: 4072 677 499 123 BbeI G_GCGC'C Cuts at: 2026 2026 Size: 5371 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 928 Size: 971 137 4263 Fragments arranged by size: 4263 971 137 BbvCI CC'TCA_GC Cuts at: 2611 2611 Size: 5371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3562 3596 1903 Size: 34 1625 34 3678 Fragments arranged by size: 3678 1625 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3263 4473 3263 Size: 1210 4161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3982 136 Size: 107 122 71 631 605 2310 1525 Fragments arranged by size: 2310 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5366 5366 Size: 5371 BlpI GC'TnA_GC Cuts at: 2788 2788 Size: 5371 Bme1580I G_kGCm'C Cuts at: 1790 2068 2339 3432 4588 1790 Size: 278 271 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 278 271 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2568 3102 4060 Size: 416 53 83 399 24 1151 534 958 Cuts at: 4060 442 Size: 1753 Fragments arranged by size: 1753 1151 958 534 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 4031 1239 Size: 1421 1371 2579 Fragments arranged by size: 2579 1421 1371 Bpu10I CC'TnA_GC Cuts at: 2611 2611 Size: 5371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 2267 3496 4361 4659 2252 Size: 15 1229 865 298 2964 Fragments arranged by size: 2964 1229 865 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 4034 882 Size: 617 609 1926 2219 Fragments arranged by size: 2219 1926 617 609 BsaAI yAC'GTr Cuts at: 493 1204 3162 493 Size: 711 1958 2702 Fragments arranged by size: 2702 1958 711 BsaBI GATnn'nnATC Cuts at: 5365 5365 Size: 5371 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2023 2033 3178 3560 Size: 53 83 186 936 490 10 1145 382 Cuts at: 3560 4195 275 Size: 635 1451 Fragments arranged by size: 1451 1145 936 635 490 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 3804 4202 4476 4623 5034 3804 Size: 398 274 147 411 4141 Fragments arranged by size: 4141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5146 Fragments arranged by size: 5146 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2438 2492 1186 Size: 868 384 54 4065 Fragments arranged by size: 4065 868 384 54 BseYI C'CCAG_C Cuts at: 2515 4574 5070 2515 Size: 2059 496 2816 Fragments arranged by size: 2816 2059 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4798 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2821 3582 4610 1540 Size: 275 186 820 761 1028 2301 Fragments arranged by size: 2301 1028 820 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3432 3517 4588 3432 Size: 85 1071 4215 Fragments arranged by size: 4215 1071 85 BsmI GAATG_Cn' Cuts at: 2882 2975 2882 Size: 93 5278 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2339 2567 2690 3432 3517 4588 Size: 399 278 271 228 123 742 85 1071 Cuts at: 4588 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 278 271 228 123 85 BspEI T'CCGG_A Cuts at: 5034 5034 Size: 5371 BspHI T'CATG_A Cuts at: 2379 3260 4990 2379 Size: 881 1730 2760 Fragments arranged by size: 2760 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2667 2726 844 Size: 1823 59 3489 Fragments arranged by size: 3489 1823 59 BsrBI CCG'CTC Cuts at: 1394 1823 2395 3258 1394 Size: 429 572 863 3507 Fragments arranged by size: 3507 863 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2133 3866 4040 65 Size: 1183 885 1733 174 1396 Fragments arranged by size: 1733 1396 1183 885 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4015 4202 1700 Size: 110 2205 187 2869 Fragments arranged by size: 2869 2205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5371 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5371 BssSI C'ACGA_G Cuts at: 3431 4443 3431 Size: 1012 4359 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5371 BstBI TT'CG_AA Cuts at: 4175 4175 Size: 5371 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5371 BstXI CCAn_nnnn'nTGG Cuts at: 2332 2332 Size: 5371 BstYI r'GATC_y Cuts at: 1972 2271 2367 2701 3068 3453 3470 4922 5008 Size: 299 96 334 367 385 17 1452 86 Cuts at: 5008 5020 5366 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 334 299 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 2454 513 Size: 552 939 327 123 3430 Fragments arranged by size: 3430 939 552 327 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4632 Fragments arranged by size: 4632 683 56 BtsI GCAGTG_nn' Cuts at: 2887 3681 3701 2887 Size: 794 20 4557 Fragments arranged by size: 4557 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5336 DraI TTT'AAA Cuts at: 2675 2675 Size: 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2284 4378 809 Size: 507 968 2094 1802 Fragments arranged by size: 2094 1802 968 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2818 3707 Size: 54 1425 50 275 18 324 664 889 Cuts at: 3707 5336 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 664 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2818 1537 Size: 275 1006 4090 Fragments arranged by size: 4090 1006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3301 1353 Size: 1116 832 3423 Fragments arranged by size: 3423 1116 832 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3958 4332 4478 387 Size: 1064 138 2369 374 146 1280 Fragments arranged by size: 2369 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2660 3434 4031 4818 5086 1239 Size: 393 743 285 774 597 787 268 1524 Fragments arranged by size: 1524 787 774 743 597 393 285 268 EcoO109I rG'GnC_Cy Cuts at: 2064 2065 2064 Size: 1 5370 EcoRI G'AATT_C Cuts at: 2680 3058 2680 Size: 378 4993 FspI TGC'GCA Cuts at: 2207 2225 3877 2207 Size: 18 1652 3701 Fragments arranged by size: 3701 1652 18 FspAI rTGC'GCAy Cuts at: 2207 2207 Size: 5371 HaeII r_GCGC'y Cuts at: 1678 2026 2218 4518 1678 Size: 348 192 2300 2531 Fragments arranged by size: 2531 2300 348 192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2525 2557 4012 4044 4086 Size: 32 135 32 953 32 1455 32 42 Cuts at: 4086 4118 1373 Size: 32 2626 Fragments arranged by size: 2626 1455 953 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2715 2961 669 Size: 988 281 777 246 3079 Fragments arranged by size: 3079 988 777 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5371 HpaI GTT'AAC Cuts at: 2961 2961 Size: 5371 KasI G'GCGC_C Cuts at: 2022 2022 Size: 5371 KpnI G_GTAC'C Cuts at: 2696 2696 Size: 5371 MfeI C'AATT_G Cuts at: 2970 2970 Size: 5371 MluI A'CGCG_T Cuts at: 5247 5247 Size: 5371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 4485 4669 1305 Size: 1008 2172 184 2007 Fragments arranged by size: 2172 2007 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 10 Size: 54 1425 343 324 3225 Fragments arranged by size: 3225 1425 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3329 3688 3847 518 Size: 648 1248 915 359 159 2042 Fragments arranged by size: 2042 1248 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2353 2787 3464 4612 4857 1606 Size: 747 434 677 1148 245 2120 Fragments arranged by size: 2120 1148 747 677 434 245 NarI GG'CG_CC Cuts at: 2023 2023 Size: 5371 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 2454 513 Size: 552 939 327 123 3430 Fragments arranged by size: 3430 939 552 327 123 NdeI CA'TA_TG Cuts at: 387 387 Size: 5371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5371 NotI GC'GGCC_GC Cuts at: 2818 2818 Size: 5371 NspI r_CATG'y Cuts at: 1132 1229 2729 4274 1132 Size: 97 1500 1545 2229 Fragments arranged by size: 2229 1545 1500 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5371 PciI A'CATG_T Cuts at: 4270 4270 Size: 5371 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 3973 PmeI GTTT'AAAC Cuts at: 2675 2675 Size: 5371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 3440 3472 4953 4985 2537 Size: 32 871 32 1481 32 2923 Fragments arranged by size: 2923 1481 871 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5371 PsiI TTA'TAA Cuts at: 2941 2941 Size: 5371 PspOMI G'GGCC_C Cuts at: 2064 2064 Size: 5371 PstI C_TGCA'G Cuts at: 830 2723 830 Size: 1893 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5371 PvuII CAG'CTG Cuts at: 2353 2353 Size: 5371 SalI G'TCGA_C Cuts at: 1936 2713 1936 Size: 777 4594 SbfI CC_TGCA'GG Cuts at: 2723 2723 Size: 5371 ScaI AGT'ACT Cuts at: 3619 5331 3619 Size: 1712 3659 SfcI C'TryA_G Cuts at: 826 1045 2719 3854 4535 4726 826 Size: 219 1674 1135 681 191 1471 Fragments arranged by size: 1674 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2024 2024 Size: 5371 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5371 SmaI CCC'GGG Cuts at: 1872 2698 1872 Size: 826 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 2282 2651 3475 4376 4638 Size: 197 926 324 15 369 824 901 262 Cuts at: 4638 820 Size: 1553 Fragments arranged by size: 1553 926 901 824 369 324 262 197 15 SnaBI TAC'GTA Cuts at: 493 3162 493 Size: 2669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5371 SphI G_CATG'C Cuts at: 1132 2729 1132 Size: 1597 3774 SspI AAT'ATT Cuts at: 5 52 3295 5 Size: 47 3243 2081 Fragments arranged by size: 3243 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5371 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 2331 2454 2810 513 Size: 552 939 321 6 123 356 3074 Fragments arranged by size: 3074 939 552 356 321 123 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2447 3398 3415 3568 3753 4198 325 Size: 1255 867 951 17 153 185 445 1498 Fragments arranged by size: 1498 1255 951 867 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3617 5329 96 Size: 274 80 33 51 1443 1640 1712 138 Fragments arranged by size: 1712 1640 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5331 5363 5331 Size: 32 5339 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5371 XbaI T'CTAG_A Cuts at: 2707 2707 Size: 5371 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2296 2086 Size: 210 5161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5371 XmaI C'CCGG_G Cuts at: 1870 2696 1870 Size: 826 4545 XmnI GAAnn'nnTTC Cuts at: 3500 3500 Size: 5371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3179 276 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI