(Circular) (Six-base) MAPSORT of: pFN21AB6176.seq Check: 4707 from: 1 to: 6523 pFN21AB6176 6523 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3061 3192 3061 Size: 131 6392 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3480 4333 278 Size: 53 83 186 936 1944 853 2468 Fragments arranged by size: 2468 1944 936 853 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 2127 2187 3866 1937 Size: 136 54 60 1679 4594 Fragments arranged by size: 4594 1679 136 60 54 Acc65I G'GTAC_C Cuts at: 2889 2917 3844 2889 Size: 28 927 5568 Fragments arranged by size: 5568 927 28 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2308 2963 4586 5970 6238 1632 Size: 539 137 655 1623 1384 268 1917 Fragments arranged by size: 1917 1623 1384 655 539 268 137 AflII C'TTAA_G Cuts at: 820 1017 2351 820 Size: 197 1334 4992 Fragments arranged by size: 4992 1334 197 AflIII A'CryG_T Cuts at: 1203 3091 5422 6399 1203 Size: 1888 2331 977 1327 Fragments arranged by size: 2331 1888 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 2099 3154 1166 Size: 933 1055 4535 Fragments arranged by size: 4535 1055 933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 2941 3410 3615 3694 4266 5838 2941 Size: 469 205 79 572 1572 3626 Fragments arranged by size: 3626 1572 572 469 205 79 ApaI G_GGCC'C Cuts at: 2024 3385 2024 Size: 1361 5162 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 3832 4058 4210 6319 1493 Size: 2339 226 152 2109 1697 Fragments arranged by size: 2339 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2766 2797 3848 1870 Size: 73 106 717 31 1051 4545 Fragments arranged by size: 4545 1051 717 106 73 31 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2431 2464 2431 Size: 33 6490 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2889 2917 2969 3633 Size: 325 217 627 273 829 28 52 664 Cuts at: 3633 3844 5299 618 Size: 211 1455 1842 Fragments arranged by size: 1842 1455 829 664 627 325 273 217 211 52 28 BanII G_rGCy'C Cuts at: 1391 2024 2262 2777 2963 3167 3327 3385 3842 Size: 633 238 515 186 204 160 58 457 Cuts at: 3842 1391 Size: 4072 Fragments arranged by size: 4072 633 515 457 238 204 186 160 58 BbeI G_GCGC'C Cuts at: 3637 3637 Size: 6523 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2321 3089 3371 928 Size: 971 422 768 282 4080 Fragments arranged by size: 4080 971 768 422 282 BbvCI CC'TCA_GC Cuts at: 3429 3596 3429 Size: 167 6356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4714 4748 1903 Size: 34 2777 34 3678 Fragments arranged by size: 3678 2777 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 1548 Size: 6523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3540 5134 Size: 107 122 71 631 605 364 1504 1594 Cuts at: 5134 136 Size: 1525 Fragments arranged by size: 1594 1525 1504 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 3289 6518 3289 Size: 3229 3294 BlpI GC'TnA_GC Cuts at: 3039 3940 3039 Size: 901 5622 Bme1580I G_kGCm'C Cuts at: 1790 2024 2063 3385 4584 5740 1790 Size: 234 39 1322 1199 1156 2573 Fragments arranged by size: 2573 1322 1199 1156 234 39 BmgBI CAC'GTC Cuts at: 1318 2643 3229 1318 Size: 1325 586 4612 Fragments arranged by size: 4612 1325 586 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2919 2920 4254 Size: 416 53 83 399 24 1502 1 1334 Cuts at: 4254 5212 442 Size: 958 1753 Fragments arranged by size: 1753 1502 1334 958 416 399 83 53 24 1 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2533 3418 2533 Size: 885 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2482 3151 3592 5183 1239 Size: 1243 669 441 1591 2579 Fragments arranged by size: 2579 1591 1243 669 441 Bpu10I CC'TnA_GC Cuts at: 3077 3429 3596 3077 Size: 352 167 6004 Fragments arranged by size: 6004 352 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3002 3278 3638 4648 5513 5811 3002 Size: 276 360 1010 865 298 3714 Fragments arranged by size: 3714 1010 865 360 298 276 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5186 882 Size: 617 3687 2219 Fragments arranged by size: 3687 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 6517 6517 Size: 6523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3477 3634 4330 4712 Size: 53 83 186 936 1944 157 696 382 Cuts at: 4712 5347 275 Size: 635 1451 Fragments arranged by size: 1944 1451 936 696 635 382 186 157 83 53 BsaWI w'CCGG_w Cuts at: 2489 4956 5354 5628 5775 6186 2489 Size: 2467 398 274 147 411 2826 Fragments arranged by size: 2826 2467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2632 2662 3708 3738 1178 Size: 30 165 30 1229 30 1046 30 3963 Fragments arranged by size: 3963 1229 1046 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2760 3683 1186 Size: 1084 490 923 4026 Fragments arranged by size: 4026 1084 923 490 BseYI C'CCAG_C Cuts at: 2558 2571 2608 2777 2782 3027 3384 5726 6222 Size: 13 37 169 5 245 357 2342 496 Cuts at: 6222 2558 Size: 2859 Fragments arranged by size: 2859 2342 496 357 245 169 37 13 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 1163 Size: 573 1218 4732 Fragments arranged by size: 4732 1218 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3131 3973 4734 5762 1540 Size: 275 186 1130 842 761 1028 2301 Fragments arranged by size: 2301 1130 1028 842 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2592 3018 3078 3501 4584 4669 5740 2262 Size: 330 426 60 423 1083 85 1071 3045 Fragments arranged by size: 3045 1083 1071 426 423 330 85 60 BsmI GAATG_Cn' Cuts at: 4034 4127 4034 Size: 93 6430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2063 2262 2592 2777 2963 3018 Size: 399 234 39 199 330 185 186 55 Cuts at: 3018 3078 3167 3327 3385 3501 3842 4584 4669 Size: 60 89 160 58 116 341 742 85 Cuts at: 4669 5740 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 330 234 199 186 185 160 116 89 85 60 58 55 39 BspEI T'CCGG_A Cuts at: 2489 6186 2489 Size: 3697 2826 BspHI T'CATG_A Cuts at: 3120 4412 6142 3120 Size: 1292 1730 3501 Fragments arranged by size: 3501 1730 1292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 3061 3192 3878 844 Size: 1241 976 131 686 3489 Fragments arranged by size: 3489 1241 976 686 131 BsrBI CCG'CTC Cuts at: 1394 1823 2637 2845 3378 3673 3739 4410 1394 Size: 429 814 208 533 295 66 671 3507 Fragments arranged by size: 3507 814 671 533 429 295 208 66 BsrDI GCAATG_nn' Cuts at: 65 1248 2235 5018 5192 65 Size: 1183 987 2783 174 1396 Fragments arranged by size: 2783 1396 1183 987 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2735 5167 5354 1700 Size: 110 925 2432 187 2869 Fragments arranged by size: 2869 2432 925 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 5327 5327 Size: 6523 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 2387 2708 2387 Size: 321 6202 BstYI r'GATC_y Cuts at: 1972 3289 3407 3690 3853 4220 4605 4622 6074 Size: 1317 118 283 163 367 385 17 1452 Cuts at: 6074 6160 6172 6518 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 283 163 118 86 17 12 BstZ17I GTA'TAC Cuts at: 2074 2074 Size: 6523 Bsu36I CC'TnA_GG Cuts at: 3403 3403 Size: 6523 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 2365 2673 2682 3574 513 Size: 552 939 11 350 308 9 892 3462 Fragments arranged by size: 3462 939 892 552 350 308 11 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3012 487 Size: 683 56 1786 3998 Fragments arranged by size: 3998 1786 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2217 3521 4039 4833 4853 2061 Size: 156 1304 518 794 20 3731 Fragments arranged by size: 3731 1304 794 518 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2390 2425 529 Size: 35 1826 35 4627 Fragments arranged by size: 4627 1826 35 35 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DraIII CAC_nnn'GTG Cuts at: 2927 3153 2927 Size: 226 6297 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2710 2856 3003 Size: 54 1425 50 275 18 880 146 147 Cuts at: 3003 3128 3192 3354 3696 3970 4859 6488 8 Size: 125 64 162 342 274 889 1629 43 Fragments arranged by size: 1629 1425 889 880 342 275 274 162 147 146 125 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3128 3970 1537 Size: 275 1316 842 4090 Fragments arranged by size: 4090 1316 842 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3686 4453 1353 Size: 1586 747 767 3423 Fragments arranged by size: 3423 1586 767 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2260 2260 Size: 6523 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2308 2482 2963 3151 3592 4586 Size: 393 539 137 174 481 188 441 994 Cuts at: 4586 5183 5970 6238 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 994 787 597 539 481 441 393 268 188 174 137 EcoNI CCTnn'n_nnAGG Cuts at: 2800 3317 2800 Size: 517 6006 EcoO109I rG'GnC_Cy Cuts at: 2020 2021 2146 2272 2422 2555 3381 3448 2020 Size: 1 125 126 150 133 826 67 5095 Fragments arranged by size: 5095 826 150 133 126 125 67 1 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 6523 FspI TGC'GCA Cuts at: 5029 5029 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 3637 5670 1678 Size: 1959 2033 2531 Fragments arranged by size: 2531 2033 1959 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2834 2866 3126 3158 3281 Size: 32 135 32 1262 32 260 32 123 Cuts at: 3281 3313 5164 5196 5238 5270 1373 Size: 32 1851 32 42 32 2626 Fragments arranged by size: 2626 1851 1262 260 135 123 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2440 3097 3867 4113 669 Size: 988 281 502 657 770 246 3079 Fragments arranged by size: 3079 988 770 657 502 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6523 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KasI G'GCGC_C Cuts at: 3633 3633 Size: 6523 KpnI G_GTAC'C Cuts at: 2893 2921 3848 2893 Size: 28 927 5568 Fragments arranged by size: 5568 927 28 MfeI C'AATT_G Cuts at: 3702 4122 3702 Size: 420 6103 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2715 5637 5821 1305 Size: 1410 2922 184 2007 Fragments arranged by size: 2922 2007 1410 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2712 3356 3698 10 Size: 54 1425 343 880 644 342 2835 Fragments arranged by size: 2835 1425 880 644 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2997 3154 3186 3227 4481 4840 Size: 648 933 898 157 32 41 1254 359 Cuts at: 4840 4999 518 Size: 159 2042 Fragments arranged by size: 2042 1254 933 898 648 359 159 157 41 32 MspA1I CmG'CkG Cuts at: 1606 2290 2938 3038 3428 3664 3939 4616 5764 Size: 684 648 100 390 236 275 677 1148 Cuts at: 5764 6009 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 684 677 648 390 275 245 236 100 NarI GG'CG_CC Cuts at: 3634 3634 Size: 6523 NcoI C'CATG_G Cuts at: 513 1065 2004 2365 2682 513 Size: 552 939 361 317 4354 Fragments arranged by size: 4354 939 552 361 317 NdeI CA'TA_TG Cuts at: 387 2661 387 Size: 2274 4249 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 3095 3191 3881 5426 1132 Size: 97 1866 96 690 1545 2229 Fragments arranged by size: 2229 1866 1545 690 97 96 PasI CC'CwG_GG Cuts at: 1348 2551 3247 1348 Size: 1203 696 4624 Fragments arranged by size: 4624 1203 696 PciI A'CATG_T Cuts at: 3091 5422 3091 Size: 2331 4192 PflMI CCAn_nnn'nTGG Cuts at: 1108 3253 3391 3694 1108 Size: 2145 138 303 3937 Fragments arranged by size: 3937 2145 303 138 PfoI T'CCnGG_A Cuts at: 2109 2478 2109 Size: 369 6154 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 2422 2555 3448 2272 Size: 150 133 893 5347 Fragments arranged by size: 5347 893 150 133 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6523 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PspOMI G'GGCC_C Cuts at: 2020 3381 2020 Size: 1361 5162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 6491 PstI C_TGCA'G Cuts at: 830 3875 830 Size: 3045 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2938 3038 3428 2938 Size: 100 390 6033 Fragments arranged by size: 6033 390 100 SacI G_AGCT'C Cuts at: 2262 2262 Size: 6523 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SanDI GG'GwC_CC Cuts at: 2555 2555 Size: 6523 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 4771 6483 4771 Size: 1712 4811 SfcI C'TryA_G Cuts at: 826 1045 2624 3460 3871 5006 5687 5878 826 Size: 219 1579 836 411 1135 681 191 1471 Fragments arranged by size: 1579 1471 1135 836 681 411 219 191 SfoI GGC'GCC Cuts at: 3635 3635 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 2051 2768 3850 1872 Size: 179 717 1082 4545 Fragments arranged by size: 4545 1082 717 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 3017 3293 3653 4627 5528 Size: 197 926 408 666 276 360 974 901 Cuts at: 5528 5790 820 Size: 262 1553 Fragments arranged by size: 1553 974 926 901 666 408 360 276 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 3881 1132 Size: 2749 3774 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6523 StyI C'CwwG_G Cuts at: 513 1065 2004 2365 2418 2682 2853 3390 3621 Size: 552 939 361 53 264 171 537 231 Cuts at: 3621 3962 513 Size: 341 3074 Fragments arranged by size: 3074 939 552 537 361 341 264 231 171 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 2938 3785 4550 4567 4720 4905 Size: 1255 1005 353 847 765 17 153 185 Cuts at: 4905 5350 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1005 847 765 445 353 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4769 6481 96 Size: 274 80 33 51 1443 2792 1712 138 Fragments arranged by size: 2792 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2632 2664 6483 6515 2632 Size: 32 3819 32 2640 Fragments arranged by size: 3819 2640 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3212 1316 Size: 1896 4627 XbaI T'CTAG_A Cuts at: 2137 3859 2137 Size: 1722 4801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2659 2680 2242 Size: 417 21 6085 Fragments arranged by size: 6085 417 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 2049 2766 3848 1870 Size: 179 717 1082 4545 Fragments arranged by size: 4545 1082 717 179 XmnI GAAnn'nnTTC Cuts at: 2318 4652 2318 Size: 2334 4189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3478 4331 276 Size: 53 83 186 936 1944 853 2468 Fragments arranged by size: 2468 1944 936 853 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SapI SexAI SfiI SrfI SwaI