################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BamHI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 270 GTAC GT^AC AocI 672 CCTGAGG CC^T[ACGT]AGG AspHI 1008 GAGCTC G[AT]GC[AT]^C AvaI 39 CTCGGG C^[CT]CG[GA]G AvrII 969 CCTAGG C^CTAGG BlnI 969 CCTAGG C^CTAGG BseRI 397 CTCCTC GAGGAG(10/8) BsgI 853 GTGCAG GTGCAG(16/14) BsiEI 117 CGGCCG CG[GA][CT]^CG BsiHKAI 1008 GAGCTC G[AT]GC[AT]^C BsoBI 39 CTCGGG C^[CT]CG[GA]G BssHII 612 GCGCGC G^CGCGC BssSI 859 CACGAG CACGAG(-5/-1) BstAPI 165 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 672 CCTGAGG CC^T[ACGT]AGG EaeI 117 CGGCCG [CT]^GGCC[GA] EagI 117 CGGCCG C^GGCCG EclXI 117 CGGCCG C^GGCCG Eco52I 117 CGGCCG C^GGCCG Eco81I 672 CCTGAGG CC^T[ACGT]AGG EcoNI 447 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 969 CCTAGG C^C[AT][AT]GG MaeIII 637 GTGAC ^GT[ACGT]AC PpuMI 455 AGGACCT [GA]G^G[AT]CC[CT] PstI 607 CTGCAG CTGCA^G PvuII 806 CAGCTG CAG^CTG RsaI 270 GTAC GT^AC SacI 1008 GAGCTC GAGCT^C SfaNI 929 GCATC GCATC(5/9) SfcI 607 CTGCAG C^T[GA][CT]AG StyI 969 CCTAGG C^C[AT][AT]GG TaqI 179 TCGA T^CGA Tsp45I 637 GTGAC ^GT[GC]AC TthHB8I 179 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 633 ACACGT A^C[GA][CT]GT 842 ACACGT ApaI 382 GGGCCC GGGCC^C 683 GGGCCC BfaI 961 CTAG C^TAG 970 CTAG BglI 384 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 520 GCCCGTGCGGC BsmBI 651 CGTCTC CGTCTC(1/5) 601 GAGACG BsmFI 251 GGGAC GGGAC(10/14) 108 GTCCC BspMI 311 ACCTGC ACCTGC(4/8) 458 ACCTGC BsrI 357 CCAGT ACTGG(1/-1) 414 CCAGT DpnII 495 GATC ^GATC 708 GATC EarI 829 GAAGAG CTCTTC(1/4) 1005 GAAGAG HinfI 195 GAGTC G^A[ACGT]TC 1075 GAGTC HphI 497 TCACC GGTGA(8/7) 881 TCACC Ksp632I 829 GAAGAG CTCTTC(1/4) 1005 GAAGAG MaeI 961 CTAG C^TAG 970 CTAG MboI 495 GATC ^GATC 708 GATC NdeII 495 GATC ^GATC 708 GATC PleI 195 GAGTC GAGTC(4/5) 1075 GAGTC SapI 829 GAAGAGC GCTCTTC(1/4) 1005 GAAGAGC Sau3AI 495 GATC ^GATC 708 GATC XcmI 357 CCAGTGGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 914 CCATCCCCATCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BanI BanII BbeI BbrPI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI KspI MaeII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PmaCI PmlI SacII Sau96I ScrFI SfoI TseI TspRI