(Circular) (Six-base) MAPSORT of: pFN21AE1509.seq Check: 1334 from: 1 to: 5803 pFN21AE1509 5803 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3866 5250 5518 1632 Size: 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 2638 2847 4702 5679 1203 Size: 1435 209 1855 977 1327 Fragments arranged by size: 1855 1435 1327 977 209 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 2108 2567 1166 Size: 942 459 4402 Fragments arranged by size: 4402 942 459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AlwNI CAG_nnn'CTG Cuts at: 3546 5118 3546 Size: 1572 4231 ApaI G_GGCC'C Cuts at: 2391 2692 2391 Size: 301 5502 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2044 3128 1870 Size: 73 101 1084 4545 Fragments arranged by size: 4545 1084 101 73 AvrII C'CTAG_G Cuts at: 2974 2974 Size: 5803 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2221 2397 2821 3124 Size: 325 217 627 230 204 176 424 303 Cuts at: 3124 4579 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 424 325 303 230 217 204 176 BanII G_rGCy'C Cuts at: 1391 2053 2136 2391 2692 2883 3017 3074 3122 Size: 662 83 255 301 191 134 57 48 Cuts at: 3122 1391 Size: 4072 Fragments arranged by size: 4072 662 301 255 191 134 83 57 48 BbeI G_GCGC'C Cuts at: 2021 2225 2401 2021 Size: 204 176 5423 Fragments arranged by size: 5423 204 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4832 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3994 4028 1903 Size: 34 50 34 1973 34 3678 Fragments arranged by size: 3678 1973 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2395 2531 4414 Size: 107 122 71 631 605 723 136 1883 Cuts at: 4414 136 Size: 1525 Fragments arranged by size: 1883 1525 723 631 605 136 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2391 2564 2692 3067 3864 5020 1790 Size: 601 173 128 375 797 1156 2573 Fragments arranged by size: 2573 1156 797 601 375 173 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4492 442 Size: 416 53 83 399 24 2117 958 1753 Fragments arranged by size: 2117 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3057 3057 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2576 2612 2850 4463 1239 Size: 1106 231 36 238 1613 2579 Fragments arranged by size: 2579 1613 1106 238 231 36 Bpu10I CC'TnA_GC Cuts at: 3016 3016 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 2355 2765 4466 882 Size: 617 561 295 410 1701 2219 Fragments arranged by size: 2219 1701 617 561 410 295 BsaAI yAC'GTr Cuts at: 493 1204 2158 2565 2641 2850 3594 493 Size: 711 954 407 76 209 744 2702 Fragments arranged by size: 2702 954 744 711 407 209 76 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2193 2222 2398 Size: 53 83 186 936 485 175 29 176 Cuts at: 2398 3610 3992 4627 275 Size: 1212 382 635 1451 Fragments arranged by size: 1451 1212 936 635 485 382 186 176 175 83 53 29 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 2549 2579 2970 Size: 30 165 30 1050 30 66 30 391 Cuts at: 2970 3000 1178 Size: 30 3981 Fragments arranged by size: 3981 1050 391 165 66 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 1186 Size: 1207 4596 BseYI C'CCAG_C Cuts at: 2505 2685 2910 5006 5502 2505 Size: 180 225 2096 496 2806 Fragments arranged by size: 2806 2096 496 225 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2879 1163 Size: 573 1143 4087 Fragments arranged by size: 4087 1143 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 3253 4014 5042 1540 Size: 275 186 124 1128 761 1028 2301 Fragments arranged by size: 2301 1128 1028 761 275 186 124 BsiHKAI G_wGCw'C Cuts at: 3017 3864 3949 5020 3017 Size: 847 85 1071 3800 Fragments arranged by size: 3800 1071 847 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 BsmBI CGTCTCn'nnnn_ Cuts at: 2600 2662 2600 Size: 62 5741 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2136 2391 2564 2692 2883 3017 Size: 399 263 83 255 173 128 191 134 Cuts at: 3017 3067 3074 3122 3864 3949 5020 1391 Size: 50 7 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 263 255 191 173 134 128 85 83 50 48 7 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 2472 3158 844 Size: 1481 147 686 3489 Fragments arranged by size: 3489 1481 686 147 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4447 4634 1700 Size: 110 2637 187 2869 Fragments arranged by size: 2869 2637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 2617 1925 Size: 692 5111 BssSI C'ACGA_G Cuts at: 2864 3863 4875 2864 Size: 999 1012 3792 Fragments arranged by size: 3792 1012 999 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 794 Size: 1382 4421 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstYI r'GATC_y Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798 Size: 1161 367 385 17 1452 86 12 346 Cuts at: 5798 1972 Size: 1977 Fragments arranged by size: 1977 1452 1161 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2678 2678 Size: 5803 BtgI C'CryG_G Cuts at: 513 1065 2125 2216 2225 2407 2481 513 Size: 552 1060 91 9 182 74 3835 Fragments arranged by size: 3835 1060 552 182 91 74 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 2431 3073 487 Size: 683 56 902 303 642 3217 Fragments arranged by size: 3217 902 683 642 303 56 BtsI GCAGTG_nn' Cuts at: 2154 3319 4113 4133 2154 Size: 1165 794 20 3824 Fragments arranged by size: 3824 1165 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 3250 4139 Size: 54 1425 50 275 18 292 1128 889 Cuts at: 4139 5768 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1128 889 292 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2122 3250 1537 Size: 275 310 1128 4090 Fragments arranged by size: 4090 1128 310 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2829 3005 3733 1353 Size: 1476 176 728 3423 Fragments arranged by size: 3423 1476 728 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2413 2549 4390 4764 4910 387 Size: 1064 138 824 136 1841 374 146 1280 Fragments arranged by size: 1841 1280 1064 824 374 146 138 136 EcoICRI GAG'CTC Cuts at: 3015 3015 Size: 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2576 2612 2850 3866 4463 5250 Size: 393 713 231 36 238 1016 597 787 Cuts at: 5250 5518 1239 Size: 268 1524 Fragments arranged by size: 1524 1016 787 713 597 393 268 238 231 36 EcoNI CCTnn'n_nnAGG Cuts at: 2456 2456 Size: 5803 EcoO109I rG'GnC_Cy Cuts at: 2387 2461 2689 2387 Size: 74 228 5501 Fragments arranged by size: 5501 228 74 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 2021 2225 2284 2401 4950 1678 Size: 343 204 59 117 2549 2531 Fragments arranged by size: 2549 2531 343 204 117 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 2453 2485 2635 Size: 32 135 32 761 32 88 32 150 Cuts at: 2635 2667 4444 4476 4518 4550 1373 Size: 32 1777 32 42 32 2626 Fragments arranged by size: 2626 1777 761 150 135 88 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KasI G'GCGC_C Cuts at: 2017 2221 2397 2017 Size: 204 176 5423 Fragments arranged by size: 5423 204 176 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2147 2567 3761 4120 4279 518 Size: 648 942 39 420 1194 359 159 2042 Fragments arranged by size: 2042 1194 942 648 420 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2127 2218 2409 2483 2731 2813 3219 3896 Size: 521 91 191 74 248 82 406 677 Cuts at: 3896 5044 5289 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 521 406 248 245 191 91 82 74 NarI GG'CG_CC Cuts at: 2018 2222 2398 2018 Size: 204 176 5423 Fragments arranged by size: 5423 204 176 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 3161 4706 1132 Size: 97 1932 1545 2229 Fragments arranged by size: 2229 1932 1545 97 PasI CC'CwG_GG Cuts at: 1348 2692 3067 1348 Size: 1344 375 4084 Fragments arranged by size: 4084 1344 375 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5803 PfoI T'CCnGG_A Cuts at: 2471 2471 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PmlI CAC'GTG Cuts at: 2565 2641 2850 2565 Size: 76 209 5518 Fragments arranged by size: 5518 209 76 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2461 2461 Size: 5803 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2387 2688 2387 Size: 301 5502 PstI C_TGCA'G Cuts at: 830 2616 3155 830 Size: 1786 539 3478 Fragments arranged by size: 3478 1786 539 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2813 2813 Size: 5803 SacI G_AGCT'C Cuts at: 3017 3017 Size: 5803 SacII CC_GC'GG Cuts at: 2128 2219 2410 2484 2128 Size: 91 191 74 5447 Fragments arranged by size: 5447 191 91 74 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SapI GCTCTTCn'nnn_ Cuts at: 2829 3005 2829 Size: 176 5627 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2612 3151 4286 4967 5158 826 Size: 219 1567 539 1135 681 191 1471 Fragments arranged by size: 1567 1471 1135 681 539 219 191 SfoI GGC'GCC Cuts at: 2019 2223 2399 2019 Size: 204 176 5423 Fragments arranged by size: 5423 204 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 2974 3242 513 Size: 552 1909 268 3074 Fragments arranged by size: 3074 1909 552 268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2218 2313 2519 2643 3830 3847 Size: 1255 498 140 95 206 124 1187 17 Cuts at: 3847 4000 4185 4630 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1187 498 445 206 185 153 140 124 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 4049 5761 Size: 274 80 33 51 1443 297 1775 1712 Cuts at: 5761 96 Size: 138 Fragments arranged by size: 1775 1712 1443 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 2970 3002 5763 5795 2549 Size: 32 389 32 2761 32 2557 Fragments arranged by size: 2761 2557 389 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2369 2926 2011 Size: 358 557 4888 Fragments arranged by size: 4888 557 358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI