(Linear) (Six-base) MAPSORT of: KIEE1509.seq Check: 6457 from: 1 to: 1098 KIEE1509.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AflIII A'CryG_T Cuts at: 0 633 842 1098 Size: 633 209 256 Fragments arranged by size: 633 256 209 AleI CACnn'nnGTG Cuts at: 0 103 562 1098 Size: 103 459 536 Fragments arranged by size: 536 459 103 ApaI G_GGCC'C Cuts at: 0 386 687 1098 Size: 386 301 411 Fragments arranged by size: 411 386 301 AvaI C'yCGr_G Cuts at: 0 39 1098 Size: 39 1059 AvrII C'CTAG_G Cuts at: 0 969 1098 Size: 969 129 BanI G'GyrC_C Cuts at: 0 12 216 392 816 1098 Size: 12 204 176 424 282 Fragments arranged by size: 424 282 204 176 12 BanII G_rGCy'C Cuts at: 0 48 131 386 687 878 1012 1069 1098 Size: 48 83 255 301 191 134 57 29 Fragments arranged by size: 301 255 191 134 83 57 48 29 BbeI G_GCGC'C Cuts at: 0 16 220 396 1098 Size: 16 204 176 702 Fragments arranged by size: 702 204 176 16 BglI GCCn_nnn'nGGC Cuts at: 0 390 526 1098 Size: 390 136 572 Fragments arranged by size: 572 390 136 Bme1580I G_kGCm'C Cuts at: 0 386 559 687 1062 1098 Size: 386 173 128 375 36 Fragments arranged by size: 386 375 173 128 36 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1052 1098 Size: 1052 46 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 571 607 845 1098 Size: 340 231 36 238 253 Fragments arranged by size: 340 253 238 231 36 Bpu10I CC'TnA_GC Cuts at: 0 1011 1098 Size: 1011 87 BsaI GGTCTCn'nnnn_ Cuts at: 0 55 350 760 1098 Size: 55 295 410 338 Fragments arranged by size: 410 338 295 55 BsaAI yAC'GTr Cuts at: 0 153 560 636 845 1098 Size: 153 407 76 209 253 Fragments arranged by size: 407 253 209 153 76 BsaHI Gr'CG_yC Cuts at: 0 13 188 217 393 1098 Size: 13 175 29 176 705 Fragments arranged by size: 705 176 175 29 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 448 478 544 574 965 995 1098 Size: 448 30 66 30 391 30 103 Fragments arranged by size: 448 391 103 66 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 388 1098 Size: 388 710 BseYI C'CCAG_C Cuts at: 0 500 680 905 1098 Size: 500 180 225 193 Fragments arranged by size: 500 225 193 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1098 Size: 874 224 BsiEI CG_ry'CG Cuts at: 0 120 1098 Size: 120 978 BsiHKAI G_wGCw'C Cuts at: 0 1012 1098 Size: 1012 86 BsmBI CGTCTCn'nnnn_ Cuts at: 0 595 657 1098 Size: 595 62 441 Fragments arranged by size: 595 441 62 Bsp1286I G_dGCh'C Cuts at: 0 48 131 386 559 687 878 1012 1062 Size: 48 83 255 173 128 191 134 50 Cuts at: 1062 1069 1098 Size: 7 29 Fragments arranged by size: 255 191 173 134 128 83 50 48 29 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 320 467 1098 Size: 320 147 631 Fragments arranged by size: 631 320 147 BssHII G'CGCG_C Cuts at: 0 612 1098 Size: 612 486 BssSI C'ACGA_G Cuts at: 0 859 1098 Size: 859 239 BstAPI GCAn_nnn'nTGC Cuts at: 0 171 1098 Size: 171 927 Bsu36I CC'TnA_GG Cuts at: 0 673 1098 Size: 673 425 BtgI C'CryG_G Cuts at: 0 120 211 220 402 476 1098 Size: 120 91 9 182 74 622 Fragments arranged by size: 622 182 120 91 74 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 123 426 1068 1098 Size: 123 303 642 30 Fragments arranged by size: 642 303 123 30 BtsI GCAGTG_nn' Cuts at: 0 149 1098 Size: 149 949 EaeI y'GGCC_r Cuts at: 0 117 1098 Size: 117 981 EagI C'GGCC_G Cuts at: 0 117 1098 Size: 117 981 EarI CTCTTCn'nnn_ Cuts at: 0 824 1000 1098 Size: 824 176 98 Fragments arranged by size: 824 176 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 408 544 1098 Size: 408 136 554 Fragments arranged by size: 554 408 136 EcoICRI GAG'CTC Cuts at: 0 1010 1098 Size: 1010 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 571 607 845 1098 Size: 340 231 36 238 253 Fragments arranged by size: 340 253 238 231 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 451 1098 Size: 451 647 EcoO109I rG'GnC_Cy Cuts at: 0 382 456 684 1098 Size: 382 74 228 414 Fragments arranged by size: 414 382 228 74 HaeII r_GCGC'y Cuts at: 0 16 220 279 396 1098 Size: 16 204 59 117 702 Fragments arranged by size: 702 204 117 59 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 328 360 448 480 630 662 1098 Size: 328 32 88 32 150 32 436 Fragments arranged by size: 436 328 150 88 32 32 32 KasI G'GCGC_C Cuts at: 0 12 216 392 1098 Size: 12 204 176 706 Fragments arranged by size: 706 204 176 12 MslI CAynn'nnrTG Cuts at: 0 103 142 562 1098 Size: 103 39 420 536 Fragments arranged by size: 536 420 103 39 MspA1I CmG'CkG Cuts at: 0 122 213 404 478 726 808 1098 Size: 122 91 191 74 248 82 290 Fragments arranged by size: 290 248 191 122 91 82 74 NarI GG'CG_CC Cuts at: 0 13 217 393 1098 Size: 13 204 176 705 Fragments arranged by size: 705 204 176 13 PasI CC'CwG_GG Cuts at: 0 687 1062 1098 Size: 687 375 36 Fragments arranged by size: 687 375 36 PfoI T'CCnGG_A Cuts at: 0 466 1098 Size: 466 632 PmlI CAC'GTG Cuts at: 0 560 636 845 1098 Size: 560 76 209 253 Fragments arranged by size: 560 253 209 76 PpuMI rG'GwC_Cy Cuts at: 0 456 1098 Size: 456 642 PspOMI G'GGCC_C Cuts at: 0 382 683 1098 Size: 382 301 415 Fragments arranged by size: 415 382 301 PstI C_TGCA'G Cuts at: 0 611 1098 Size: 611 487 PvuII CAG'CTG Cuts at: 0 808 1098 Size: 808 290 SacI G_AGCT'C Cuts at: 0 1012 1098 Size: 1012 86 SacII CC_GC'GG Cuts at: 0 123 214 405 479 1098 Size: 123 91 191 74 619 Fragments arranged by size: 619 191 123 91 74 SapI GCTCTTCn'nnn_ Cuts at: 0 824 1000 1098 Size: 824 176 98 Fragments arranged by size: 824 176 98 SfcI C'TryA_G Cuts at: 0 607 1098 Size: 607 491 SfoI GGC'GCC Cuts at: 0 14 218 394 1098 Size: 14 204 176 704 Fragments arranged by size: 704 204 176 14 StyI C'CwwG_G Cuts at: 0 969 1098 Size: 969 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 73 213 308 514 638 1098 Size: 73 140 95 206 124 460 Fragments arranged by size: 460 206 140 124 95 73 TatI w'GTAC_w Cuts at: 0 269 1098 Size: 269 829 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 544 576 965 997 1098 Size: 544 32 389 32 101 Fragments arranged by size: 544 389 101 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 364 921 1098 Size: 364 557 177 Fragments arranged by size: 557 364 177 Enzymes that do cut: AflIII AleI ApaI AvaI AvrII BanI BanII BbeI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BssHII BssSI BstAPI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI PasI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI