(Circular) (Six-base) MAPSORT of: pFN21AE3615.seq Check: 2671 from: 1 to: 5725 pFN21AE3615 5725 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3024 3852 4225 1563 Size: 1461 828 373 3063 Fragments arranged by size: 3063 1461 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 2361 2498 4624 5601 1203 Size: 1158 137 2126 977 1327 Fragments arranged by size: 2126 1327 1158 977 137 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 2658 4454 2658 Size: 1796 3929 AleI CACnn'nnGTG Cuts at: 1166 2556 2828 1166 Size: 1390 272 4063 Fragments arranged by size: 4063 1390 272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 2785 3468 5040 2785 Size: 683 1572 3470 Fragments arranged by size: 3470 1572 683 ApaLI G'TGCA_C Cuts at: 2273 3782 4938 2273 Size: 1509 1156 3060 Fragments arranged by size: 3060 1509 1156 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2485 3050 1870 Size: 73 542 565 4545 Fragments arranged by size: 4545 565 542 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 2738 3044 1391 Size: 1347 306 4072 Fragments arranged by size: 4072 1347 306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2540 928 Size: 971 641 4113 Fragments arranged by size: 4113 971 641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2277 3786 4942 1790 Size: 487 1509 1156 2573 Fragments arranged by size: 2573 1509 1156 487 BmgBI CAC'GTC Cuts at: 1318 2826 1318 Size: 1508 4217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2200 2532 3456 Size: 416 53 83 399 24 783 332 924 Cuts at: 3456 4414 442 Size: 958 1753 Fragments arranged by size: 1753 958 924 783 416 399 332 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4385 1239 Size: 3146 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 3850 4715 5013 2030 Size: 1820 865 298 2742 Fragments arranged by size: 2742 1820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2499 3516 493 Size: 711 1295 1017 2702 Fragments arranged by size: 2702 1295 1017 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 1186 Size: 1098 4627 BseYI C'CCAG_C Cuts at: 4928 5424 4928 Size: 496 5229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2713 1163 Size: 573 977 4175 Fragments arranged by size: 4175 977 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3786 3871 4942 2277 Size: 1509 85 1071 3060 Fragments arranged by size: 3060 1509 1071 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2738 3044 3786 3871 4942 1391 Size: 399 487 461 306 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 461 399 306 85 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 2304 3614 5344 2304 Size: 1310 1730 2685 Fragments arranged by size: 2685 1730 1310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 2775 3080 844 Size: 1819 112 305 3489 Fragments arranged by size: 3489 1819 305 112 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2404 2893 4220 4394 65 Size: 1183 1156 489 1327 174 1396 Fragments arranged by size: 1396 1327 1183 1156 489 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2678 2801 3785 4797 2678 Size: 123 984 1012 3606 Fragments arranged by size: 3606 1012 984 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 2814 794 Size: 2020 3705 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720 Size: 1083 367 385 17 1452 86 12 346 Cuts at: 5720 1972 Size: 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2829 513 Size: 552 939 825 3409 Fragments arranged by size: 3409 939 825 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2285 3241 4035 4055 2285 Size: 956 794 20 3955 Fragments arranged by size: 3955 956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DraIII CAC_nnn'GTG Cuts at: 2499 2499 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2674 2848 Size: 54 1425 50 275 18 339 505 174 Cuts at: 2848 2932 2985 3172 4061 5690 8 Size: 84 53 187 889 1629 43 Fragments arranged by size: 1629 1425 889 505 339 275 187 174 84 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 3655 1353 Size: 1250 1052 3423 Fragments arranged by size: 3423 1250 1052 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2164 4312 4686 4832 387 Size: 1064 138 575 2148 374 146 1280 Fragments arranged by size: 2148 1280 1064 575 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3788 4385 5172 5440 1239 Size: 393 2156 597 787 268 1524 Fragments arranged by size: 2156 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2610 2652 2610 Size: 42 5683 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 4872 1678 Size: 3194 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2644 2676 2709 Size: 32 135 32 518 32 522 32 33 Cuts at: 2709 2741 4366 4398 4440 4472 1373 Size: 32 1625 32 42 32 2626 Fragments arranged by size: 2626 1625 522 518 135 42 33 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 4839 5023 1305 Size: 1359 2175 184 2007 Fragments arranged by size: 2175 2007 1359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 2676 2934 2987 10 Size: 54 1425 343 339 505 258 53 2748 Fragments arranged by size: 2748 1425 505 343 339 258 54 53 MslI CAynn'nnrTG Cuts at: 518 1166 2556 2828 3683 4042 4201 518 Size: 648 1390 272 855 359 159 2042 Fragments arranged by size: 2042 1390 855 648 359 272 159 MspA1I CmG'CkG Cuts at: 1606 2969 3141 3818 4966 5211 1606 Size: 1363 172 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 677 245 172 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2346 2365 3083 4628 1132 Size: 97 1117 19 718 1545 2229 Fragments arranged by size: 2229 1545 1117 718 97 19 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 2361 4624 2361 Size: 2263 3462 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 2634 1108 Size: 1082 444 4199 Fragments arranged by size: 4199 1082 444 PfoI T'CCnGG_A Cuts at: 2457 2457 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PmlI CAC'GTG Cuts at: 2499 2499 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2652 2652 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 2819 3077 830 Size: 1989 258 3478 Fragments arranged by size: 3478 1989 258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2969 2969 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2815 3073 4208 4889 5080 826 Size: 219 1770 258 1135 681 191 1471 Fragments arranged by size: 1770 1471 1135 681 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3829 4730 4992 820 Size: 197 926 102 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2473 3649 5 Size: 47 2421 1176 2081 Fragments arranged by size: 2421 2081 1176 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 513 Size: 552 939 1160 3074 Fragments arranged by size: 3074 1160 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 2348 2380 2483 2515 5685 5717 2062 Size: 32 254 32 103 32 3170 32 2070 Fragments arranged by size: 3170 2070 254 103 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI