(Circular) (Six-base) MAPSORT of: pFN21AE4487.seq Check: 4888 from: 1 to: 6229 pFN21AE4487 6229 bp With 182 enzymes: SgfI * March 28, 2012 13:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3168 4039 278 Size: 53 83 186 936 1632 871 2468 Fragments arranged by size: 2468 1632 936 871 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 4292 5676 5944 1632 Size: 914 1746 1384 268 1917 Fragments arranged by size: 1917 1746 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 2083 2308 2368 3972 5544 2083 Size: 225 60 1604 1572 2768 Fragments arranged by size: 2768 1604 1572 225 60 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2162 2804 2997 3052 3359 3538 3764 3916 Size: 669 642 193 55 307 179 226 152 Cuts at: 3916 6025 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 669 642 307 226 193 179 152 55 AseI AT'TA_AT Cuts at: 160 1032 2493 4783 160 Size: 872 1461 2290 1606 Fragments arranged by size: 2290 1606 1461 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 3554 1870 Size: 73 1611 4545 Fragments arranged by size: 4545 1611 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3550 5005 618 Size: 325 217 627 1763 1455 1842 Fragments arranged by size: 1842 1763 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2523 3548 1391 Size: 622 510 1025 4072 Fragments arranged by size: 4072 1025 622 510 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5258 BbvCI CC'TCA_GC Cuts at: 3464 3464 Size: 6229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2868 4121 5331 2868 Size: 1253 1210 3766 Fragments arranged by size: 3766 1253 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2439 6224 2439 Size: 3785 2444 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 4290 5446 1790 Size: 2500 1156 2573 Fragments arranged by size: 2573 2500 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2636 3960 4918 Size: 416 53 83 399 24 1219 1324 958 Cuts at: 4918 442 Size: 1753 Fragments arranged by size: 1753 1324 1219 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 4889 1239 Size: 866 2784 2579 Fragments arranged by size: 2784 2579 866 Bpu10I CC'TnA_GC Cuts at: 3464 3464 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 4354 5219 5517 2240 Size: 2114 865 298 2952 Fragments arranged by size: 2952 2114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3404 4892 882 Size: 617 1905 1488 2219 Fragments arranged by size: 2219 1905 1488 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 2967 6223 2967 Size: 3256 2973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3165 4036 4418 5053 Size: 53 83 186 936 1632 871 382 635 Cuts at: 5053 275 Size: 1451 Fragments arranged by size: 1632 1451 936 871 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3384 3414 1178 Size: 30 165 30 1981 30 3993 Fragments arranged by size: 3993 1981 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3219 3481 1186 Size: 2033 262 3934 Fragments arranged by size: 3934 2033 262 BseYI C'CCAG_C Cuts at: 5432 5928 5432 Size: 496 5733 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 1163 Size: 573 390 5266 Fragments arranged by size: 5266 573 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2050 2054 3679 4440 5468 1540 Size: 275 186 49 4 1625 761 1028 2301 Fragments arranged by size: 2301 1625 1028 761 275 186 49 4 BsiHKAI G_wGCw'C Cuts at: 2013 2271 2523 4290 4375 5446 2013 Size: 258 252 1767 85 1071 2796 Fragments arranged by size: 2796 1767 1071 258 252 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2271 2523 3548 4290 4375 5446 Size: 399 223 258 252 1025 742 85 1071 Cuts at: 5446 1391 Size: 2174 Fragments arranged by size: 2174 1071 1025 742 399 258 252 223 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 3584 844 Size: 1888 852 3489 Fragments arranged by size: 3489 1888 852 BsrBI CCG'CTC Cuts at: 1394 1823 4116 1394 Size: 429 2293 3507 Fragments arranged by size: 3507 2293 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 3166 4724 4898 65 Size: 1183 1479 439 1558 174 1396 Fragments arranged by size: 1558 1479 1396 1183 439 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 4873 5060 1700 Size: 110 239 2824 187 2869 Fragments arranged by size: 2869 2824 239 187 110 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 4153 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 2393 794 Size: 1599 4630 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2443 3223 2443 Size: 780 5449 BstYI r'GATC_y Cuts at: 1972 2439 2476 3559 3926 4311 4328 5780 5866 Size: 467 37 1083 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1083 467 385 367 346 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2514 513 Size: 552 939 510 4228 Fragments arranged by size: 4228 939 552 510 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 2365 487 Size: 683 56 896 243 4351 Fragments arranged by size: 4351 896 683 243 56 BtsI GCAGTG_nn' Cuts at: 2562 3745 4539 4559 2562 Size: 1183 794 20 4232 Fragments arranged by size: 4232 1183 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3294 3533 3294 Size: 239 5990 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2133 2716 Size: 54 1425 50 275 18 217 86 583 Cuts at: 2716 3676 4565 6194 8 Size: 960 889 1629 43 Fragments arranged by size: 1629 1425 960 889 583 275 217 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2047 3676 1537 Size: 275 235 1629 4090 Fragments arranged by size: 4090 1629 275 235 EarI CTCTTCn'nnn_ Cuts at: 1353 4159 1353 Size: 2806 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2521 2011 Size: 510 5719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2546 4292 4889 5676 5944 1239 Size: 393 473 441 1746 597 787 268 1524 Fragments arranged by size: 1746 1524 787 597 473 441 393 268 EcoO109I rG'GnC_Cy Cuts at: 2089 3475 2089 Size: 1386 4843 EcoRI G'AATT_C Cuts at: 2162 3538 3916 2162 Size: 1376 378 4475 Fragments arranged by size: 4475 1376 378 EcoRV GAT'ATC Cuts at: 2125 3323 2125 Size: 1198 5031 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 5376 1678 Size: 3698 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 3122 3154 4870 Size: 32 135 32 1267 32 251 32 1716 Cuts at: 4870 4902 4944 4976 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1716 1267 251 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 748 Size: 1702 4527 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2053 5343 5527 1305 Size: 748 3290 184 2007 Fragments arranged by size: 3290 2007 748 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 10 Size: 54 1425 343 886 3521 Fragments arranged by size: 3521 1425 886 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4187 4546 4705 518 Size: 648 3021 359 159 2042 Fragments arranged by size: 3021 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 3434 3645 4322 5470 5715 1606 Size: 477 1351 211 677 1148 245 2120 Fragments arranged by size: 2120 1351 1148 677 477 245 211 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4738 Fragments arranged by size: 4738 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NruI TCG'CGA Cuts at: 2056 2056 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 2405 3799 2405 Size: 1394 4835 PstI C_TGCA'G Cuts at: 830 2079 2389 3581 830 Size: 1249 310 1192 3478 Fragments arranged by size: 3478 1249 1192 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2083 2083 Size: 6229 SacI G_AGCT'C Cuts at: 2013 2523 2013 Size: 510 5719 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 2955 4477 6189 2955 Size: 1522 1712 2995 Fragments arranged by size: 2995 1712 1522 SfcI C'TryA_G Cuts at: 826 1045 2075 2385 3577 4712 5393 5584 826 Size: 219 1030 310 1192 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 1030 681 310 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 4333 5234 5496 820 Size: 197 926 276 2114 901 262 1553 Fragments arranged by size: 2114 1553 926 901 276 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 2288 1517 Size: 771 5458 StyI C'CwwG_G Cuts at: 513 1065 2004 2197 2979 3668 513 Size: 552 939 193 782 689 3074 Fragments arranged by size: 3074 939 782 689 552 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 4256 4273 4426 4611 5056 325 Size: 1255 1806 870 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 2953 4475 Size: 274 80 33 51 1443 195 781 1522 Cuts at: 4475 6187 96 Size: 1712 138 Fragments arranged by size: 1712 1522 1443 781 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 6189 6221 2382 Size: 32 3775 32 2390 Fragments arranged by size: 3775 2390 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 3377 4358 3377 Size: 981 5248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3166 4037 276 Size: 53 83 186 936 1632 871 2468 Fragments arranged by size: 2468 1632 936 871 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI