Restriction map for
pFN21AE4372
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmFI BsmI Bsp1407I BspEI BspHI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoT14I EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 28 CGCG CG^CG
AflIII 461 ACATGT A^C[GA][CT]GT
ApaI 832 GGGCCC GGGCC^C
Asp700I 913 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 163 GTGCTC G[AT]GC[AT]^C
AvaI 377 CCCGGG C^[CT]CG[GA]G
BfaI 535 CTAG C^TAG
BglI 349 GCCTACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 820 AGATCT A^GATCT
BsiEI 1061 CGGCCG CG[GA][CT]^CG
BsiHKAI 163 GTGCTC G[AT]GC[AT]^C
BsiYI 1139 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1139 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 22 GTCTC GTCTC(1/5)
BsmBI 21 CGTCTC CGTCTC(1/5)
BsoBI 377 CCCGGG C^[CT]CG[GA]G
BspDI 453 ATCGAT AT^CGAT
BspLU11I 461 ACATGT A^CATGT
BspMI 507 GCAGGT ACCTGC(4/8)
BsrBI 704 GAGCGG CCGCTC(-3/-3)
BsrDI 488 GCAATG GCAATG(2/0)
BssSI 908 CACGAG CACGAG(-5/-1)
BstAPI 91 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 613 TTCGAA TT^CGAA
BstUI 28 CGCG CG^CG
BstXI 836 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 820 AGATCT [GA]^GATC[CT]
ClaI 453 ATCGAT AT^CGAT
DrdI 886 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1061 CGGCCG [CT]^GGCC[GA]
EagI 1061 CGGCCG C^GGCCG
EarI 1084 GAAGAG CTCTTC(1/4)
EclXI 1061 CGGCCG C^GGCCG
Eco52I 1061 CGGCCG C^GGCCG
EcoRV 772 GATATC GAT^ATC
FauI 26 CCCGC CCCGC(4/6)
HgaI 782 GCGTC GACGC(5/10)
HinfI 893 GAGTC G^A[ACGT]TC
Ksp632I 1084 GAAGAG CTCTTC(1/4)
MaeI 535 CTAG C^TAG
MaeII 691 ACGT A^CGT
MflI 820 AGATCT [GA]^GATC[CT]
MspA1I 238 CAGCTG C[AC]G^C[GT]G
MvnI 28 CGCG CG^CG
NspI 461 ACATGT [GA]CATG^[CT]
NspV 613 TTCGAA TT^CGAA
PflMI 1139 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 893 GAGTC GAGTC(4/5)
PstI 850 CTGCAG CTGCA^G
PvuII 238 CAGCTG CAG^CTG
SapI 1084 GAAGAGC GCTCTTC(1/4)
SfuI 613 TTCGAA TT^CGAA
SmaI 377 CCCGGG CCC^GGG
SmlI 958 CTCAAG C^T[CT][GA]AG
Van91I 1139 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 820 AGATCT [GA]^GATC[CT]
XmaCI 377 CCCGGG C^CCGGG
XmaI 377 CCCGGG C^CCGGG
XmnI 913 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 398 AAATTC [GA]^AATT[CT]
917 AAATTC
AfaI 211 GTAC GT^AC
905 GTAC
ApoI 398 AAATTC [GA]^AATT[CT]
917 AAATTC
BanI 510 GGTGCC G^G[CT][GA]CC
1135 GGTGCC
BcgI 585 CGATGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
488 GCAATGGCATCG
BsrI 989 CCAGT ACTGG(1/-1)
1050 CCAGT
DsaI 867 CCACGG C^C[GA][CT]GG
1058 CCACGG
HaeII 40 GGCGCT [GA]GCGC^[CT]
520 GGCGCT
HindIII 293 AAGCTT A^AGCTT
617 AAGCTT
MseI 113 TTAA T^TAA
1157 TTAA
NaeI 54 GCCGGC GCC^GGC
316 GCCGGC
NgoMIV 54 GCCGGC G^CCGGC
316 GCCGGC
NlaIII 462 CATG CATG^
627 CATG
RsaI 211 GTAC GT^AC
905 GTAC
SfcI 192 CTACAG C^T[GA][CT]AG
850 CTGCAG
TaqI 454 TCGA T^CGA
614 TCGA
Tru9I 113 TTAA T^TAA
1157 TTAA
TthHB8I 454 TCGA T^CGA
614 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BcnI BmyI BpmI BsaJI Bsp1286I
BsrFI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI