(Circular) (Six-base) MAPSORT of: pFN21AE4372.seq Check: 1408 from: 1 to: 5875 pFN21AE4372 5875 bp With 182 enzymes: SgfI * March 28, 2012 14:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2503 2503 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3938 5322 5590 1632 Size: 2306 1384 268 1917 Fragments arranged by size: 2306 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2466 4774 5751 1203 Size: 1263 2308 977 1327 Fragments arranged by size: 2308 1327 1263 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 2165 2283 1166 Size: 999 118 4758 Fragments arranged by size: 4758 999 118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2487 1184 Size: 1303 4572 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaI G_GGCC'C Cuts at: 2841 2841 Size: 5875 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2403 2922 3184 3410 3562 5671 1493 Size: 910 519 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 910 519 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2382 3200 1870 Size: 73 439 818 4545 Fragments arranged by size: 4545 818 439 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2515 3140 3196 4651 618 Size: 325 217 627 728 625 56 1455 1842 Fragments arranged by size: 1842 1455 728 627 625 325 217 56 BanII G_rGCy'C Cuts at: 1391 2190 2365 2841 3194 1391 Size: 799 175 476 353 4072 Fragments arranged by size: 4072 799 476 353 175 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2482 2516 2579 2613 4066 4100 1903 Size: 34 545 34 63 34 1453 34 3678 Fragments arranged by size: 3678 1453 545 63 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 4486 136 Size: 107 122 71 631 605 688 2126 1525 Fragments arranged by size: 2126 1525 688 631 605 122 107 71 BglII A'GATC_T Cuts at: 2825 5870 2825 Size: 3045 2830 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2520 2841 3936 5092 1790 Size: 730 321 1095 1156 2573 Fragments arranged by size: 2573 1156 1095 730 321 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3049 3606 4564 Size: 416 53 83 399 24 1632 557 958 Cuts at: 4564 442 Size: 1753 Fragments arranged by size: 1753 1632 958 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 2423 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2916 3097 4535 1239 Size: 1097 580 181 1438 2579 Fragments arranged by size: 2579 1438 1097 580 181 Bpu10I CC'TnA_GC Cuts at: 3075 3075 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2948 4000 4865 5163 2948 Size: 1052 865 298 3660 Fragments arranged by size: 3660 1052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2499 2529 3095 3125 1178 Size: 30 165 30 1096 30 566 30 3928 Fragments arranged by size: 3928 1096 566 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5875 BseYI C'CCAG_C Cuts at: 2528 3094 5078 5574 2528 Size: 566 1984 496 2829 Fragments arranged by size: 2829 1984 566 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3069 3325 4086 5114 1540 Size: 275 186 1068 256 761 1028 2301 Fragments arranged by size: 2301 1068 1028 761 275 256 186 BsiHKAI G_wGCw'C Cuts at: 2172 3936 4021 5092 2172 Size: 1764 85 1071 2955 Fragments arranged by size: 2955 1764 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 BsmBI CGTCTCn'nnnn_ Cuts at: 2032 2032 Size: 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2190 2365 2520 2841 3194 3936 Size: 399 382 18 175 155 321 353 742 Cuts at: 3936 4021 5092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 353 321 175 155 85 18 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2503 3230 844 Size: 1659 727 3489 Fragments arranged by size: 3489 1659 727 BsrBI CCG'CTC Cuts at: 1394 1823 2711 3762 1394 Size: 429 888 1051 3507 Fragments arranged by size: 3507 1051 888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2500 4370 4544 65 Size: 1183 1252 1870 174 1396 Fragments arranged by size: 1870 1396 1252 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2136 2321 4519 4706 1700 Size: 110 249 77 185 2198 187 2869 Fragments arranged by size: 2869 2198 249 187 185 110 77 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2913 3935 4947 2913 Size: 1022 1012 3841 Fragments arranged by size: 3841 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2102 794 Size: 1308 4567 BstBI TT'CG_AA Cuts at: 2619 4679 2619 Size: 2060 3815 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2848 2848 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2825 3205 3572 3957 3974 5426 5512 5524 Size: 853 380 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 853 385 380 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2872 3063 513 Size: 552 1807 191 3325 Fragments arranged by size: 3325 1807 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2604 487 Size: 683 56 1378 3758 Fragments arranged by size: 3758 1378 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 2459 1554 Size: 905 4970 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2897 4882 809 Size: 507 1581 1985 1802 Fragments arranged by size: 1985 1802 1581 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3066 3322 4211 Size: 54 1425 50 275 18 1236 256 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1236 889 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3066 3322 1537 Size: 275 1254 256 4090 Fragments arranged by size: 4090 1254 275 256 EarI CTCTTCn'nnn_ Cuts at: 1353 3084 3805 1353 Size: 1731 721 3423 Fragments arranged by size: 3423 1731 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2916 3097 3938 4535 5322 5590 Size: 393 704 580 181 841 597 787 268 Cuts at: 5590 1239 Size: 1524 Fragments arranged by size: 1524 841 787 704 597 580 393 268 181 EcoO109I rG'GnC_Cy Cuts at: 2150 2386 2837 2150 Size: 236 451 5188 Fragments arranged by size: 5188 451 236 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 EcoRV GAT'ATC Cuts at: 2779 2779 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2049 2529 5022 1678 Size: 371 480 2493 2531 Fragments arranged by size: 2531 2493 480 371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373 Size: 32 135 32 2944 32 42 32 2626 Fragments arranged by size: 2944 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 2298 2622 748 Size: 1550 324 4001 Fragments arranged by size: 4001 1550 324 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2283 2469 2846 3833 4192 4351 Size: 648 999 118 186 377 987 359 159 Cuts at: 4351 518 Size: 2042 Fragments arranged by size: 2042 999 987 648 377 359 186 159 118 MspA1I CmG'CkG Cuts at: 1606 2245 3291 3968 5116 5361 1606 Size: 639 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 639 245 NaeI GCC'GGC Cuts at: 2061 2323 2061 Size: 262 5613 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5323 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NgoMIV G'CCGG_C Cuts at: 2059 2321 2059 Size: 262 5613 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2470 3233 4778 1132 Size: 97 1241 763 1545 2229 Fragments arranged by size: 2229 1545 1241 763 97 PasI CC'CwG_GG Cuts at: 1348 2521 1348 Size: 1173 4702 PciI A'CATG_T Cuts at: 2466 4774 2466 Size: 2308 3567 PflMI CCAn_nnn'nTGG Cuts at: 1108 3150 1108 Size: 2042 3833 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2837 2837 Size: 5875 PstI C_TGCA'G Cuts at: 830 2859 3227 830 Size: 2029 368 3478 Fragments arranged by size: 3478 2029 368 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2245 2245 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 3084 3084 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2197 2855 3223 4358 5039 5230 826 Size: 219 1152 658 368 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 681 658 368 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2384 3202 1872 Size: 512 818 4545 Fragments arranged by size: 4545 818 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 2963 3979 4880 5142 820 Size: 197 926 1020 1016 901 262 1553 Fragments arranged by size: 1553 1020 1016 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 3314 513 Size: 552 2249 3074 Fragments arranged by size: 3074 2249 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2499 2531 2768 2800 3055 3087 3095 3127 5835 Size: 32 237 32 255 32 8 32 2708 Cuts at: 5835 5867 2499 Size: 32 2507 Fragments arranged by size: 2708 2507 255 237 32 32 32 32 32 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2382 3200 1870 Size: 512 818 4545 Fragments arranged by size: 4545 818 512 XmnI GAAnn'nnTTC Cuts at: 2922 4004 2922 Size: 1082 4793 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI