################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 174 CGCG CG^CG AcyI 763 GGCGCC G[GA]^CG[CT]C Alw44I 633 GTGCAC G^TGCAC AlwNI 731 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 28 AGCGCT AGC^GCT ApaLI 633 GTGCAC G^TGCAC AspHI 633 GTGCAC G[AT]GC[AT]^C AspI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 763 GGCGCC G^G[CT][GA]CC BbeI 763 GGCGCC GGCGC^C BfaI 88 CTAG C^TAG BglII 198 AGATCT A^GATCT BsaHI 763 GGCGCC G[GA]^CG[CT]C BsaI 214 GAGACC GGTCTC(1/5) BsaWI 426 TCCGGT [AT]^CCGG[AT] BsiHKAI 633 GTGCAC G[AT]GC[AT]^C Bsp1407I 479 TGTACA T^GTACA BsrGI 479 TGTACA T^GTACA BsrI 679 ACTGG ACTGG(1/-1) BstAPI 730 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 174 CGCG CG^CG BstXI 353 CCACGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 91 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 749 CCATGG C^C[GA][CT]GG Eco47III 28 AGCGCT AGC^GCT EcoO109I 91 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 169 GATATC GAT^ATC FauI 767 CCCGC CCCGC(4/6) HgaI 413 GCGTC GACGC(5/10) Hin1I 763 GGCGCC G[GA]^CG[CT]C HincII 574 GTCAAC GT[CT]^[GA]AC HindII 574 GTCAAC GT[CT]^[GA]AC HphI 147 GGTGA GGTGA(8/7) KasI 763 GGCGCC G^GCGCC MaeI 88 CTAG C^TAG MaeII 578 ACGT A^CGT MvnI 174 CGCG CG^CG NarI 763 GGCGCC GG^CGCC NcoI 749 CCATGG C^CATGG PaeR7I 569 CTCGAG C^TCGAG PflFI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC SfoI 763 GGCGCC GGC^GCC SmaI 326 CCCGGG CCC^GGG SmlI 569 CTCGAG C^T[CT][GA]AG SspBI 479 TGTACA T^GTACA StuI 971 AGGCCT AGG^CCT Tsp509I 433 AATT ^AATT Tth111I 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 740 CCACCCTCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 569 CTCGAG C^TCGAG XmaCI 326 CCCGGG C^CCGGG XmaI 326 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 480 GTAC GT^AC 854 GTAC AvaII 244 GGACC G^G[AT]CC 660 GGACC BalI 362 TGGCCA TGG^CCA 594 TGGCCA BamHI 561 GGATCC G^GATCC 686 GGATCC BmyI 383 GTGCCC G[AGT]GC[ACT]^C 633 GTGCAC BpmI 98 CTGGAG CTGGAG(16/14) 299 CTGGAG BseRI 924 CTCCTC GAGGAG(10/8) 934 GAGGAG BsmAI 214 GAGAC GTCTC(1/5) 922 GTCTC Bsp1286I 383 GTGCCC G[AGT]GC[ACT]^C 633 GTGCAC BsrDI 417 CATTGC GCAATG(2/0) 793 GCAATG BsrFI 697 GCCGGC [GA]^CCGG[CT] 760 GCCGGC Cfr10I 697 GCCGGC [GA]^CCGG[CT] 760 GCCGGC DdeI 202 CTGAG C^T[ACGT]AG 629 CTCAG HaeII 28 AGCGCT [GA]GCGC^[CT] 763 GGCGCC HinfI 302 GAGTC G^A[ACGT]TC 572 GAGTC MaeIII 505 GTGAC ^GT[ACGT]AC 837 GTCAC MluNI 362 TGGCCA TGG^CCA 594 TGGCCA MscI 362 TGGCCA TGG^CCA 594 TGGCCA MseI 431 TTAA T^TAA 833 TTAA MslI 354 CACGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 702 CACGGAGGTG NaeI 697 GCCGGC GCC^GGC 760 GCCGGC NgoMIV 697 GCCGGC G^CCGGC 760 GCCGGC PleI 302 GAGTC GAGTC(4/5) 572 GAGTC PvuII 253 CAGCTG CAG^CTG 664 CAGCTG RsaI 480 GTAC GT^AC 854 GTAC Tru9I 431 TTAA T^TAA 833 TTAA Tsp45I 505 GTGAC ^GT[GC]AC 837 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaI BbsI BbvI BcnI BpuAI BsaJI BsiYI BslI BsmFI BsoBI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I XhoII