################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AccII AccIII AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MfeI MflI MluI MroI MseI MunI MvnI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 625 GACGTC GACGT^C Acc65I 905 GGTACC G^GTACC AccI 706 GTCTAC GT^[AC][GT]AC AclI 789 AACGTT AA^CGTT AflIII 8 ACGTGT A^C[GA][CT]GT AlwI 965 GGATC GGATC(4/5) AocI 194 CCTCAGG CC^T[ACGT]AGG ApaI 504 GGGCCC GGGCC^C Asp718I 905 GGTACC G^GTACC BalI 527 TGGCCA TGG^CCA BbeI 133 GGCGCC GGCGC^C BbvCI 360 CCTCAGC CCTCAGC(-5/-2) BcgI 959 GCAACTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 748 CTAG C^TAG BsaBI 525 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 511 GGTCTC GGTCTC(1/5) BsaWI 260 ACCGGA [AT]^CCGG[AT] BsmBI 894 CGTCTC CGTCTC(1/5) BstXI 284 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 194 CCTCAGG CC^T[ACGT]AGG DpnII 966 GATC ^GATC EaeI 527 TGGCCA [CT]^GGCC[GA] EarI 937 CTCTTC CTCTTC(1/4) Eco81I 194 CCTCAGG CC^T[ACGT]AGG EcoNI 918 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 893 GCGTC GACGC(5/10) KasI 133 GGCGCC G^GCGCC KpnI 905 GGTACC GGTAC^C Ksp632I 937 CTCTTC CTCTTC(1/4) MaeI 748 CTAG C^TAG MamI 525 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 966 GATC ^GATC MluNI 527 TGGCCA TGG^CCA MscI 527 TGGCCA TGG^CCA NarI 133 GGCGCC GG^CGCC NdeI 401 CATATG CA^TATG NdeII 966 GATC ^GATC NspI 806 GCATGC [GA]CATG^[CT] Psp1406I 789 AACGTT AA^CGTT PstI 844 CTGCAG CTGCA^G Sau3AI 966 GATC ^GATC SfcI 844 CTGCAG C^T[GA][CT]AG SfiI 1017 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 133 GGCGCC GGC^GCC SgrAI 712 CACCGGCG C[GA]^CCGG[CT]G SphI 806 GCATGC GCATG^C XcmI 711 CCACCGGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 133 GGCGCC G[GA]^CG[CT]C 625 GACGTC AvaI 172 CCCGGG C^[CT]CG[GA]G 237 CCCGGG BanII 504 GGGCCC G[GA]GC[CT]^C 585 GGGCTC BsaHI 133 GGCGCC G[GA]^CG[CT]C 625 GACGTC BsmFI 409 GGGAC GGGAC(10/14) 425 GTCCC BsoBI 172 CCCGGG C^[CT]CG[GA]G 237 CCCGGG BssSI 64 CTCGTG CACGAG(-5/-1) 631 CTCGTG DrdI 537 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 886 GACACCAGCGTC EcoT14I 923 CCAAGG C^C[AT][AT]GG 1020 CCTTGG FauI 31 CCCGC CCCGC(4/6) 802 GCGGG HaeII 133 GGCGCC [GA]GCGC^[CT] 190 AGCGCC Hin1I 133 GGCGCC G[GA]^CG[CT]C 625 GACGTC HincII 313 GTCAAC GT[CT]^[GA]AC 343 GTCAAC HindII 313 GTCAAC GT[CT]^[GA]AC 343 GTCAAC MboII 987 GAAGA GAAGA(8/7) 938 TCTTC NaeI 57 GCCGGC GCC^GGC 136 GCCGGC NgoMIV 57 GCCGGC G^CCGGC 136 GCCGGC SfaNI 879 GATGC GCATC(5/9) 671 GCATC SmaI 172 CCCGGG CCC^GGG 237 CCCGGG StyI 923 CCAAGG C^C[AT][AT]GG 1020 CCTTGG TfiI 644 GAATC G^A[AT]TC 1035 GAATC XmaCI 172 CCCGGG C^CCGGG 237 CCCGGG XmaI 172 CCCGGG C^CCGGG 237 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI TaqI TseI Tsp45I TspRI TthHB8I