(Circular) (Six-base) MAPSORT of: pFN21AE4346.seq Check: 5360 from: 1 to: 5788 pFN21AE4346 5788 bp With 182 enzymes: SgfI * March 28, 2012 13:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2560 2842 2560 Size: 282 5506 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2634 3598 278 Size: 53 83 186 936 1098 964 2468 Fragments arranged by size: 2468 1098 964 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2712 3131 1937 Size: 775 419 4594 Fragments arranged by size: 4594 775 419 Acc65I G'GTAC_C Cuts at: 2910 3109 2910 Size: 199 5589 AclI AA'CG_TT Cuts at: 1563 2795 3915 4288 1563 Size: 1232 1120 373 3063 Fragments arranged by size: 3063 1232 1120 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3851 5235 5503 1632 Size: 2219 1384 268 1917 Fragments arranged by size: 2219 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 2013 4687 5664 1203 Size: 810 2674 977 1327 Fragments arranged by size: 2674 1327 977 810 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2900 1184 Size: 1716 4072 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaI G_GGCC'C Cuts at: 2513 2513 Size: 5788 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 3097 3323 3475 5584 1493 Size: 1604 226 152 2109 1697 Fragments arranged by size: 2109 1697 1604 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2242 3113 1870 Size: 73 234 65 871 4545 Fragments arranged by size: 4545 871 234 73 65 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2144 2910 2978 3109 Size: 325 217 627 351 6 766 68 131 Cuts at: 3109 4564 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 766 627 351 325 217 131 68 6 BanII G_rGCy'C Cuts at: 1391 2513 2594 3107 1391 Size: 1122 81 513 4072 Fragments arranged by size: 4072 1122 513 81 BbeI G_GCGC'C Cuts at: 2142 2142 Size: 5788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BbvCI CC'TCA_GC Cuts at: 2366 2366 Size: 5788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2953 2987 3979 4013 1903 Size: 34 1016 34 992 34 3678 Fragments arranged by size: 3678 1016 992 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2041 2204 2808 Size: 107 122 71 631 605 369 163 604 Cuts at: 2808 3029 4399 136 Size: 221 1370 1525 Fragments arranged by size: 1525 1370 631 605 604 369 221 163 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2249 2513 2741 3849 5005 1790 Size: 459 264 228 1108 1156 2573 Fragments arranged by size: 2573 1156 1108 459 264 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 2748 3008 4448 1239 Size: 1415 94 260 1440 2579 Fragments arranged by size: 2579 1440 1415 260 94 Bpu10I CC'TnA_GC Cuts at: 2366 2490 2366 Size: 124 5664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 4451 882 Size: 617 1023 1929 2219 Fragments arranged by size: 2219 1929 1023 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 2534 5782 2534 Size: 3248 2540 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2631 3595 3977 Size: 53 83 186 936 606 492 964 382 Cuts at: 3977 4612 275 Size: 635 1451 Fragments arranged by size: 1451 964 936 635 606 492 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 4221 4619 4893 5040 5451 2265 Size: 1956 398 274 147 411 2602 Fragments arranged by size: 2602 1956 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2694 2724 1178 Size: 30 165 30 1291 30 4242 Fragments arranged by size: 4242 1291 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2747 2930 1186 Size: 1084 477 183 4044 Fragments arranged by size: 4044 1084 477 183 BseYI C'CCAG_C Cuts at: 2122 2506 2544 2756 4991 5487 2122 Size: 384 38 212 2235 496 2423 Fragments arranged by size: 2423 2235 496 384 212 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3849 3934 5005 3849 Size: 85 1071 4632 Fragments arranged by size: 4632 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 BsmBI CGTCTCn'nnnn_ Cuts at: 2905 2905 Size: 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2249 2513 2594 2741 3107 3849 3934 Size: 399 459 264 81 147 366 742 85 Cuts at: 3934 5005 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 459 399 366 264 147 85 81 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 2842 2931 3143 844 Size: 1716 282 89 212 3489 Fragments arranged by size: 3489 1716 282 212 89 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2141 2718 4432 4619 1700 Size: 110 252 79 577 1714 187 2869 Fragments arranged by size: 2869 1714 577 252 187 110 79 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 2069 2636 3848 4860 2069 Size: 567 1212 1012 2997 Fragments arranged by size: 2997 1212 1012 567 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5788 BstYI r'GATC_y Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783 Size: 1146 367 385 17 1452 86 12 346 Cuts at: 5783 1972 Size: 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2200 2200 Size: 5788 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2548 2897 4795 809 Size: 507 1232 349 1898 1802 Fragments arranged by size: 1898 1802 1232 507 349 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 3235 4124 Size: 54 1425 50 275 18 702 703 889 Cuts at: 4124 5753 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 703 702 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2948 3718 1353 Size: 1595 770 3423 Fragments arranged by size: 3423 1595 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4375 4749 4895 387 Size: 1064 138 490 2296 374 146 1280 Fragments arranged by size: 2296 1280 1064 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 2748 3008 3851 4448 5235 5503 Size: 393 1022 94 260 843 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1022 843 787 597 393 268 260 94 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2927 Size: 5788 EcoO109I rG'GnC_Cy Cuts at: 2220 2510 3030 2220 Size: 290 520 4978 Fragments arranged by size: 4978 520 290 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2142 2199 4935 1678 Size: 464 57 2736 2531 Fragments arranged by size: 2736 2531 464 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2694 2726 4429 4461 4503 Size: 32 135 32 1122 32 1703 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 1703 1122 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 2350 3132 3378 669 Size: 988 281 382 30 782 246 3079 Fragments arranged by size: 3079 988 782 382 281 246 30 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KasI G'GCGC_C Cuts at: 2138 2138 Size: 5788 KpnI G_GTAC'C Cuts at: 2914 3113 2914 Size: 199 5589 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 4902 5086 1305 Size: 1104 2493 184 2007 Fragments arranged by size: 2493 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 10 Size: 54 1425 343 702 3264 Fragments arranged by size: 3264 1425 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2408 2744 2882 3746 4105 4264 Size: 648 1128 114 336 138 864 359 159 Cuts at: 4264 518 Size: 2042 Fragments arranged by size: 2042 1128 864 648 359 336 159 138 114 MspA1I CmG'CkG Cuts at: 1606 2122 2425 2506 2848 2917 3204 3881 5029 Size: 516 303 81 342 69 287 677 1148 Cuts at: 5029 5274 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 516 342 303 287 245 81 69 NaeI GCC'GGC Cuts at: 2064 2143 2064 Size: 79 5709 NarI GG'CG_CC Cuts at: 2139 2139 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 NdeI CA'TA_TG Cuts at: 387 2407 387 Size: 2020 3768 NgoMIV G'CCGG_C Cuts at: 2062 2141 2062 Size: 79 5709 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2815 3146 4691 1132 Size: 97 1586 331 1545 2229 Fragments arranged by size: 2229 1586 1545 331 97 PasI CC'CwG_GG Cuts at: 1348 2617 2695 1348 Size: 1269 78 4441 Fragments arranged by size: 4441 1269 78 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 2178 3857 3889 5370 5402 2146 Size: 32 1679 32 1481 32 2532 Fragments arranged by size: 2532 1679 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2509 2509 Size: 5788 PstI C_TGCA'G Cuts at: 830 2853 3140 830 Size: 2023 287 3478 Fragments arranged by size: 3478 2023 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2425 2506 2848 2917 2425 Size: 81 342 69 5296 Fragments arranged by size: 5296 342 81 69 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2849 3136 4271 4952 5143 826 Size: 219 1804 287 1135 681 191 1471 Fragments arranged by size: 1804 1471 1135 681 287 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3029 3029 Size: 5788 SfoI GGC'GCC Cuts at: 2140 2140 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 2718 1700 Size: 1018 4770 SmaI CCC'GGG Cuts at: 1872 2179 2244 3115 1872 Size: 307 65 871 4545 Fragments arranged by size: 4545 871 307 65 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 2815 3146 1132 Size: 1683 331 3774 Fragments arranged by size: 3774 1683 331 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 2928 3025 3227 513 Size: 552 939 924 97 202 3074 Fragments arranged by size: 3074 939 924 552 202 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2447 2687 2846 2966 3815 3832 3985 Size: 1255 867 240 159 120 849 17 153 Cuts at: 3985 4170 4615 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 867 849 445 240 185 159 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2449 4034 5746 Size: 274 80 33 51 1443 472 1585 1712 Cuts at: 5746 96 Size: 138 Fragments arranged by size: 1712 1585 1443 472 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2962 2994 5748 5780 2962 Size: 32 2754 32 2970 Fragments arranged by size: 2970 2754 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2723 2723 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2177 2242 3113 1870 Size: 307 65 871 4545 Fragments arranged by size: 4545 871 307 65 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2632 3596 276 Size: 53 83 186 936 1098 964 2468 Fragments arranged by size: 2468 1098 964 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI