################################## # NO CUT ENZYMES ( 216 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I TseI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflIII 216 ACATGT A^C[GA][CT]GT AluI 345 AGCT AG^CT AlwI 269 GGATC GGATC(4/5) AvaII 3 GGTCC G^G[AT]CC BfaI 59 CTAG C^TAG BmyI 144 GTGCCC G[AGT]GC[ACT]^C BseRI 292 GAGGAG GAGGAG(10/8) BsiYI 285 CCTTATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 285 CCTTATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 66 GTCCC GGGAC(10/14) Bsp1286I 144 GTGCCC G[AGT]GC[ACT]^C BspLU11I 216 ACATGT A^CATGT Cac8I 276 GCGTGC GC[ACGT]^[ACGT]GC DpnII 270 GATC ^GATC DraII 226 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoNI 285 CCTTATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 226 AGGGCCT [GA]G^G[ACGT]CC[CT] HaeIII 228 GGCC GG^CC HinfI 336 GAGTC G^A[ACGT]TC MaeI 59 CTAG C^TAG MaeII 24 ACGT A^CGT MaeIII 263 GTGAC ^GT[ACGT]AC MboI 270 GATC ^GATC NdeII 270 GATC ^GATC NlaIV 238 GGTTCC GG[ACGT]^[ACGT]CC PleI 336 GAGTC GAGTC(4/5) Sau3AI 270 GATC ^GATC Tsp45I 263 GTGAC ^GT[GC]AC Tsp509I 87 AATT ^AATT ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsaJI 75 CCCTGG C^C[ACGT][ACGT]GG 147 CCCAGG BssKI 76 CCTGG ^CC[ACGT]GG 148 CCAGG BstNI 76 CCTGG CC^[AT]GG 148 CCAGG Cfr13I 3 GGTCC G^G[ACGT]CC 227 GGGCC EcoRII 76 CCTGG ^CC[AT]GG 148 CCAGG MvaI 76 CCTGG CC^[AT]GG 148 CCAGG NspI 133 GCATGT [GA]CATG^[CT] 216 ACATGT Sau96I 3 GGTCC G^G[ACGT]CC 227 GGGCC ScrFI 76 CCTGG CC^[ACGT]GG 148 CCAGG TaqI 159 TCGA T^CGA 290 TCGA TthHB8I 159 TCGA T^CGA 290 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI NlaIII