(Circular) (Six-base) MAPSORT of: pFN21AB6876.seq Check: 2901 from: 1 to: 5305 pFN21AB6876 5305 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3115 278 Size: 53 83 186 936 1579 2468 Fragments arranged by size: 2468 1579 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2648 1937 Size: 711 4594 Acc65I G'GTAC_C Cuts at: 2097 2626 2097 Size: 529 4776 AclI AA'CG_TT Cuts at: 1563 3432 3805 1563 Size: 1869 373 3063 Fragments arranged by size: 3063 1869 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 3368 4752 5020 1632 Size: 500 1236 1384 268 1917 Fragments arranged by size: 1917 1384 1236 500 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5108 AflIII A'CryG_T Cuts at: 1203 4204 5181 1203 Size: 3001 977 1327 Fragments arranged by size: 3001 1327 977 AgeI A'CCGG_T Cuts at: 4136 4136 Size: 5305 AhdI GACnn_n'nnGTC Cuts at: 4034 4034 Size: 5305 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5305 AlwNI CAG_nnn'CTG Cuts at: 2308 3048 4620 2308 Size: 740 1572 2993 Fragments arranged by size: 2993 1572 740 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 5305 ApaLI G'TGCA_C Cuts at: 3362 4518 3362 Size: 1156 4149 ApoI r'AATT_y Cuts at: 1493 2516 2547 2614 2840 2992 5101 1493 Size: 1023 31 67 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 226 152 67 31 AseI AT'TA_AT Cuts at: 160 1032 3859 160 Size: 872 2827 1606 Fragments arranged by size: 2827 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 AvaI C'yCGr_G Cuts at: 1870 1943 2408 2630 1870 Size: 73 465 222 4545 Fragments arranged by size: 4545 465 222 73 BamHI G'GATC_C Cuts at: 2635 3002 2635 Size: 367 4938 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2240 2626 4081 618 Size: 325 217 627 310 143 386 1455 1842 Fragments arranged by size: 1842 1455 627 386 325 310 217 143 BanII G_rGCy'C Cuts at: 1391 2029 2624 1391 Size: 638 595 4072 Fragments arranged by size: 4072 638 595 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3496 3530 1903 Size: 34 1559 34 3678 Fragments arranged by size: 3678 1559 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3197 4407 3197 Size: 1210 4095 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 4453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3916 136 Size: 107 122 71 631 605 2244 1525 Fragments arranged by size: 2244 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2466 5300 2466 Size: 2834 2471 BlpI GC'TnA_GC Cuts at: 2722 2722 Size: 5305 Bme1580I G_kGCm'C Cuts at: 1790 2029 3366 4522 1790 Size: 239 1337 1156 2573 Fragments arranged by size: 2573 1337 1156 239 BmgBI CAC'GTC Cuts at: 1318 2335 2416 1318 Size: 1017 81 4207 Fragments arranged by size: 4207 1017 81 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3036 3994 442 Size: 416 53 83 399 24 1619 958 1753 Fragments arranged by size: 1753 1619 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5305 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2107 2107 Size: 5305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2330 2393 3965 1239 Size: 827 264 63 1572 2579 Fragments arranged by size: 2579 1572 827 264 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3430 4295 4593 3430 Size: 865 298 4142 Fragments arranged by size: 4142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3968 882 Size: 617 2469 2219 Fragments arranged by size: 2469 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3096 493 Size: 711 1892 2702 Fragments arranged by size: 2702 1892 711 BsaBI GATnn'nnATC Cuts at: 5299 5299 Size: 5305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3112 3494 4129 275 Size: 53 83 186 936 1579 382 635 1451 Fragments arranged by size: 1579 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3738 4136 4410 4557 4968 3738 Size: 398 274 147 411 4075 Fragments arranged by size: 4075 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 1178 Size: 30 165 30 669 30 4381 Fragments arranged by size: 4381 669 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2093 2096 1186 Size: 877 30 3 4395 Fragments arranged by size: 4395 877 30 3 BseYI C'CCAG_C Cuts at: 2139 2202 4508 5004 2139 Size: 63 2306 496 2440 Fragments arranged by size: 2440 2306 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 1163 Size: 573 522 4210 Fragments arranged by size: 4210 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2131 2755 3516 4544 1540 Size: 275 186 130 624 761 1028 2301 Fragments arranged by size: 2301 1028 761 624 275 186 130 BsiHKAI G_wGCw'C Cuts at: 2093 3366 3451 4522 2093 Size: 1273 85 1071 2876 Fragments arranged by size: 2876 1273 1071 85 BsmI GAATG_Cn' Cuts at: 2816 2909 2816 Size: 93 5212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2093 2624 3366 3451 4522 1391 Size: 399 239 64 531 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 239 85 64 BspEI T'CCGG_A Cuts at: 4968 4968 Size: 5305 BspHI T'CATG_A Cuts at: 3194 4924 3194 Size: 1730 3575 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2660 844 Size: 1816 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3192 1394 Size: 429 1369 3507 Fragments arranged by size: 3507 1369 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3800 3974 65 Size: 1183 2552 174 1396 Fragments arranged by size: 2552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 3949 4136 1700 Size: 110 316 1823 187 2869 Fragments arranged by size: 2869 1823 316 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5305 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5305 BssSI C'ACGA_G Cuts at: 3365 4377 3365 Size: 1012 4293 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5305 BstBI TT'CG_AA Cuts at: 4109 4109 Size: 5305 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5305 BstYI r'GATC_y Cuts at: 1972 2040 2466 2635 3002 3387 3404 4856 4942 Size: 68 426 169 367 385 17 1452 86 Cuts at: 4942 4954 5300 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 169 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2286 513 Size: 552 939 282 3532 Fragments arranged by size: 3532 939 552 282 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2251 487 Size: 683 56 1025 3541 Fragments arranged by size: 3541 1025 683 56 BtsI GCAGTG_nn' Cuts at: 2821 3615 3635 2821 Size: 794 20 4491 Fragments arranged by size: 4491 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5270 DraI TTT'AAA Cuts at: 2609 2609 Size: 5305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4312 809 Size: 507 2996 1802 Fragments arranged by size: 2996 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2535 2752 Size: 54 1425 50 275 18 298 407 217 Cuts at: 2752 3641 5270 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 407 298 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2128 2752 1537 Size: 275 316 624 4090 Fragments arranged by size: 4090 624 316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3235 1353 Size: 1882 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3892 4266 4412 387 Size: 1064 138 2303 374 146 1280 Fragments arranged by size: 2303 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2132 2330 2393 3368 3965 4752 Size: 393 434 66 198 63 975 597 787 Cuts at: 4752 5020 1239 Size: 268 1524 Fragments arranged by size: 1524 975 787 597 434 393 268 198 66 63 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 5305 EcoO109I rG'GnC_Cy Cuts at: 2025 2026 2025 Size: 1 5304 EcoRI G'AATT_C Cuts at: 2516 2614 2992 2516 Size: 98 378 4829 Fragments arranged by size: 4829 378 98 FspI TGC'GCA Cuts at: 3811 3811 Size: 5305 HaeII r_GCGC'y Cuts at: 1678 4452 1678 Size: 2774 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373 Size: 32 135 32 2374 32 42 32 2626 Fragments arranged by size: 2626 2374 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2649 2895 669 Size: 988 281 711 246 3079 Fragments arranged by size: 3079 988 711 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5305 HpaI GTT'AAC Cuts at: 2895 2895 Size: 5305 KpnI G_GTAC'C Cuts at: 2101 2630 2101 Size: 529 4776 MfeI C'AATT_G Cuts at: 2904 2904 Size: 5305 MluI A'CGCG_T Cuts at: 5181 5181 Size: 5305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 4419 4603 1305 Size: 948 2166 184 2007 Fragments arranged by size: 2166 2007 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 10 Size: 54 1425 343 705 2778 Fragments arranged by size: 2778 1425 705 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3263 3622 3781 518 Size: 648 2097 359 159 2042 Fragments arranged by size: 2097 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2288 2721 3398 4546 4791 1606 Size: 453 229 433 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 453 433 245 229 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3814 Fragments arranged by size: 3814 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5305 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5305 NotI GC'GGCC_GC Cuts at: 2752 2752 Size: 5305 NspI r_CATG'y Cuts at: 1132 1229 2210 2663 4208 1132 Size: 97 981 453 1545 2229 Fragments arranged by size: 2229 1545 981 453 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5305 PciI A'CATG_T Cuts at: 4204 4204 Size: 5305 PflMI CCAn_nnn'nTGG Cuts at: 1108 2308 1108 Size: 1200 4105 PfoI T'CCnGG_A Cuts at: 2043 2043 Size: 5305 PmeI GTTT'AAAC Cuts at: 2609 2609 Size: 5305 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3374 3406 4887 4919 3374 Size: 32 1481 32 3760 Fragments arranged by size: 3760 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5305 PsiI TTA'TAA Cuts at: 2875 2875 Size: 5305 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 5305 PstI C_TGCA'G Cuts at: 830 2058 2657 830 Size: 1228 599 3478 Fragments arranged by size: 3478 1228 599 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5305 PvuII CAG'CTG Cuts at: 2059 2059 Size: 5305 SacII CC_GC'GG Cuts at: 2289 2289 Size: 5305 SalI G'TCGA_C Cuts at: 1936 2647 1936 Size: 711 4594 SbfI CC_TGCA'GG Cuts at: 2657 2657 Size: 5305 ScaI AGT'ACT Cuts at: 2477 3553 5265 2477 Size: 1076 1712 2517 Fragments arranged by size: 2517 1712 1076 SfcI C'TryA_G Cuts at: 826 1045 2054 2653 3788 4469 4660 826 Size: 219 1009 599 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 599 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5305 SmaI CCC'GGG Cuts at: 1872 2632 1872 Size: 760 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3409 4310 4572 820 Size: 197 926 1466 901 262 1553 Fragments arranged by size: 1553 1466 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3096 493 Size: 2603 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5305 SphI G_CATG'C Cuts at: 1132 2663 1132 Size: 1531 3774 SspI AAT'ATT Cuts at: 5 52 3229 5 Size: 47 3177 2081 Fragments arranged by size: 3177 2081 47 StuI AGG'CCT Cuts at: 1517 2195 1517 Size: 678 4627 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2190 2744 513 Size: 552 939 30 156 554 3074 Fragments arranged by size: 3074 939 554 552 156 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 3332 3349 3502 3687 4132 325 Size: 1255 573 1179 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1179 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 3551 5263 Size: 274 80 33 51 1443 498 1076 1712 Cuts at: 5263 96 Size: 138 Fragments arranged by size: 1712 1443 1076 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 5265 5297 2072 Size: 32 3161 32 2080 Fragments arranged by size: 3161 2080 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5305 XbaI T'CTAG_A Cuts at: 2641 2641 Size: 5305 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2041 2305 2041 Size: 264 5041 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5305 XmaI C'CCGG_G Cuts at: 1870 2630 1870 Size: 760 4545 XmnI GAAnn'nnTTC Cuts at: 2516 3434 2516 Size: 918 4387 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3113 276 Size: 53 83 186 936 1579 2468 Fragments arranged by size: 2468 1579 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI