(Linear) (Six-base) MAPSORT of: KIEE1377.seq Check: 609 from: 1 to: 1578 KIEE1377.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 169 182 440 693 1380 1578 Size: 69 100 13 258 253 687 198 Fragments arranged by size: 687 258 253 198 100 69 13 AflII C'TTAA_G Cuts at: 0 1366 1578 Size: 1366 212 AhdI GACnn_n'nnGTC Cuts at: 0 1134 1578 Size: 1134 444 AlwNI CAG_nnn'CTG Cuts at: 0 26 1578 Size: 26 1552 ApaLI G'TGCA_C Cuts at: 0 1066 1578 Size: 1066 512 AvaI C'yCGr_G Cuts at: 0 1102 1187 1578 Size: 1102 85 391 Fragments arranged by size: 1102 391 85 AvrII C'CTAG_G Cuts at: 0 594 1578 Size: 594 984 BamHI G'GATC_C Cuts at: 0 899 1578 Size: 899 679 BanI G'GyrC_C Cuts at: 0 1206 1578 Size: 1206 372 BanII G_rGCy'C Cuts at: 0 75 341 461 717 776 1109 1154 1578 Size: 75 266 120 256 59 333 45 424 Fragments arranged by size: 424 333 266 256 120 75 59 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 987 1099 1226 1541 1578 Size: 987 112 127 315 37 Fragments arranged by size: 987 315 127 112 37 BbvCI CC'TCA_GC Cuts at: 0 908 1361 1578 Size: 908 453 217 Fragments arranged by size: 908 453 217 BglII A'GATC_T Cuts at: 0 704 1578 Size: 704 874 BlpI GC'TnA_GC Cuts at: 0 572 674 1174 1578 Size: 572 102 500 404 Fragments arranged by size: 572 500 404 102 Bme1580I G_kGCm'C Cuts at: 0 1070 1211 1578 Size: 1070 141 367 Fragments arranged by size: 1070 367 141 BmrI ACTGGGnnnn_n' Cuts at: 0 837 1578 Size: 837 741 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 253 948 1578 Size: 253 695 630 Fragments arranged by size: 695 630 253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 760 1168 1447 1578 Size: 325 435 408 279 131 Fragments arranged by size: 435 408 325 279 131 Bpu10I CC'TnA_GC Cuts at: 0 908 1361 1578 Size: 908 453 217 Fragments arranged by size: 908 453 217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1045 1578 Size: 1045 533 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 1136 1166 1578 Size: 91 30 1015 30 412 Fragments arranged by size: 1015 412 91 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 947 1578 Size: 947 631 BseYI C'CCAG_C Cuts at: 0 951 986 1578 Size: 951 35 592 Fragments arranged by size: 951 592 35 BsiEI CG_ry'CG Cuts at: 0 611 1578 Size: 611 967 BsiHKAI G_wGCw'C Cuts at: 0 776 1070 1154 1578 Size: 776 294 84 424 Fragments arranged by size: 776 424 294 84 Bsp1286I G_dGCh'C Cuts at: 0 75 341 461 717 776 1070 1109 1154 Size: 75 266 120 256 59 294 39 45 Cuts at: 1154 1211 1578 Size: 57 367 Fragments arranged by size: 367 294 266 256 120 75 59 57 45 39 BspHI T'CATG_A Cuts at: 0 1088 1578 Size: 1088 490 BsrDI GCAATG_nn' Cuts at: 0 377 825 1203 1578 Size: 377 448 378 375 Fragments arranged by size: 448 378 377 375 BsrGI T'GTAC_A Cuts at: 0 137 1578 Size: 137 1441 BssSI C'ACGA_G Cuts at: 0 441 1578 Size: 441 1137 BstEII G'GTnAC_C Cuts at: 0 1291 1578 Size: 1291 287 BstYI r'GATC_y Cuts at: 0 329 704 899 1578 Size: 329 375 195 679 Fragments arranged by size: 679 375 329 195 Bsu36I CC'TnA_GG Cuts at: 0 1022 1578 Size: 1022 556 BtgI C'CryG_G Cuts at: 0 833 1578 Size: 833 745 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 499 1578 Size: 499 1079 BtsI GCAGTG_nn' Cuts at: 0 218 1578 Size: 218 1360 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 593 628 1578 Size: 593 35 950 Fragments arranged by size: 950 593 35 DraIII CAC_nnn'GTG Cuts at: 0 738 1578 Size: 738 840 EaeI y'GGCC_r Cuts at: 0 437 578 1578 Size: 437 141 1000 Fragments arranged by size: 1000 437 141 EarI CTCTTCn'nnn_ Cuts at: 0 764 1010 1399 1578 Size: 764 246 389 179 Fragments arranged by size: 764 389 246 179 EcoICRI GAG'CTC Cuts at: 0 774 1152 1578 Size: 774 378 426 Fragments arranged by size: 774 426 378 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 169 182 325 440 693 760 1168 Size: 69 100 13 143 115 253 67 408 Cuts at: 1168 1380 1447 1578 Size: 212 67 131 Fragments arranged by size: 408 253 212 143 131 115 100 69 67 67 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 10 598 1578 Size: 10 588 980 Fragments arranged by size: 980 588 10 EcoO109I rG'GnC_Cy Cuts at: 0 99 1422 1578 Size: 99 1323 156 Fragments arranged by size: 1323 156 99 FspI TGC'GCA Cuts at: 0 1542 1578 Size: 1542 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 123 625 657 891 923 1120 1152 Size: 91 32 502 32 234 32 197 32 Cuts at: 1152 1578 Size: 426 Fragments arranged by size: 502 426 234 197 91 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 249 968 1578 Size: 249 719 610 Fragments arranged by size: 719 610 249 MscI TGG'CCA Cuts at: 0 439 580 1578 Size: 439 141 998 Fragments arranged by size: 998 439 141 MslI CAynn'nnrTG Cuts at: 0 445 838 1578 Size: 445 393 740 Fragments arranged by size: 740 445 393 MspA1I CmG'CkG Cuts at: 0 49 315 576 673 990 1578 Size: 49 266 261 97 317 588 Fragments arranged by size: 588 317 266 261 97 49 NspI r_CATG'y Cuts at: 0 138 1578 Size: 138 1440 PfoI T'CCnGG_A Cuts at: 0 95 109 1124 1578 Size: 95 14 1015 454 Fragments arranged by size: 1015 454 95 14 PpuMI rG'GwC_Cy Cuts at: 0 99 1422 1578 Size: 99 1323 156 Fragments arranged by size: 1323 156 99 PvuII CAG'CTG Cuts at: 0 315 576 673 990 1578 Size: 315 261 97 317 588 Fragments arranged by size: 588 317 315 261 97 SacI G_AGCT'C Cuts at: 0 776 1154 1578 Size: 776 378 424 Fragments arranged by size: 776 424 378 SapI GCTCTTCn'nnn_ Cuts at: 0 764 1010 1578 Size: 764 246 568 Fragments arranged by size: 764 568 246 SfcI C'TryA_G Cuts at: 0 1113 1547 1578 Size: 1113 434 31 Fragments arranged by size: 1113 434 31 SmaI CCC'GGG Cuts at: 0 1104 1578 Size: 1104 474 SmlI C'TyrA_G Cuts at: 0 1060 1366 1578 Size: 1060 306 212 Fragments arranged by size: 1060 306 212 SrfI GCCC'GGGC Cuts at: 0 1104 1578 Size: 1104 474 StuI AGG'CCT Cuts at: 0 175 1578 Size: 175 1403 StyI C'CwwG_G Cuts at: 0 594 1296 1316 1578 Size: 594 702 20 262 Fragments arranged by size: 702 594 262 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 699 705 1279 1578 Size: 699 6 574 299 Fragments arranged by size: 699 574 299 6 TatI w'GTAC_w Cuts at: 0 137 1076 1578 Size: 137 939 502 Fragments arranged by size: 939 502 137 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1477 1509 1578 Size: 1477 32 69 Fragments arranged by size: 1477 69 32 XmaI C'CCGG_G Cuts at: 0 1102 1578 Size: 1102 476 Enzymes that do cut: AcuI AflII AhdI AlwNI ApaLI AvaI AvrII BamHI BanI BanII BbsI BbvCI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MscI MslI MspA1I NspI PfoI PpuMI PvuII SacI SapI SfcI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI BaeI BbeI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstZ17I ClaI DraI DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI