Restriction map for
pFN21AB5403
(Linear) (Six-base) MAPSORT of: KIBB5403.seq Check: 3772 from: 1 to: 2739
KIBB5403.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2030 2739
Size: 2030 709
AccI GT'mk_AC
Cuts at: 0 2054 2739
Size: 2054 685
Acc65I G'GTAC_C
Cuts at: 0 1745 2739
Size: 1745 994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 2739
Size: 527 2212
AfeI AGC'GCT
Cuts at: 0 1462 2739
Size: 1462 1277
AflIII A'CryG_T
Cuts at: 0 1696 2205 2426 2739
Size: 1696 509 221 313
Fragments arranged by size:
1696 509 313 221
AgeI A'CCGG_T
Cuts at: 0 19 2739
Size: 19 2720
AhdI GACnn_n'nnGTC
Cuts at: 0 33 1908 2739
Size: 33 1875 831
Fragments arranged by size:
1875 831 33
AleI CACnn'nnGTG
Cuts at: 0 1837 2739
Size: 1837 902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1525 1855 2739
Size: 1525 330 884
Fragments arranged by size:
1525 884 330
AlwNI CAG_nnn'CTG
Cuts at: 0 1311 1947 2175 2562 2739
Size: 1311 636 228 387 177
Fragments arranged by size:
1311 636 387 228 177
ApaLI G'TGCA_C
Cuts at: 0 2452 2739
Size: 2452 287
ApoI r'AATT_y
Cuts at: 0 1360 2739
Size: 1360 1379
AseI AT'TA_AT
Cuts at: 0 2480 2739
Size: 2480 259
AvaI C'yCGr_G
Cuts at: 0 487 2308 2739
Size: 487 1821 431
Fragments arranged by size:
1821 487 431
BamHI G'GATC_C
Cuts at: 0 1688 2739
Size: 1688 1051
BanI G'GyrC_C
Cuts at: 0 777 1745 2000 2635 2739
Size: 777 968 255 635 104
Fragments arranged by size:
968 777 635 255 104
BanII G_rGCy'C
Cuts at: 0 65 254 1585 2546 2739
Size: 65 189 1331 961 193
Fragments arranged by size:
1331 961 193 189 65
BbeI G_GCGC'C
Cuts at: 0 2004 2739
Size: 2004 735
BbsI GAAGACnn'nnnn_
Cuts at: 0 692 2254 2739
Size: 692 1562 485
Fragments arranged by size:
1562 692 485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 512 2739
Size: 478 34 2227
Fragments arranged by size:
2227 478 34
BclI T'GATC_A
Cuts at: 0 2126 2474 2739
Size: 2126 348 265
Fragments arranged by size:
2126 348 265
BglI GCCn_nnn'nGGC
Cuts at: 0 2224 2705 2739
Size: 2224 481 34
Fragments arranged by size:
2224 481 34
BlpI GC'TnA_GC
Cuts at: 0 112 2739
Size: 112 2627
Bme1580I G_kGCm'C
Cuts at: 0 1483 2456 2638 2739
Size: 1483 973 182 101
Fragments arranged by size:
1483 973 182 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 699 2739
Size: 699 2040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1602 2739
Size: 1602 1137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1489 2056 2065 2098 2739
Size: 1489 567 9 33 641
Fragments arranged by size:
1489 641 567 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 2739
Size: 744 1995
BsaI GGTCTCn'nnnn_
Cuts at: 0 2651 2739
Size: 2651 88
BsaAI yAC'GTr
Cuts at: 0 1407 2739
Size: 1407 1332
BsaBI GATnn'nnATC
Cuts at: 0 1228 2739
Size: 1228 1511
BsaHI Gr'CG_yC
Cuts at: 0 1106 2001 2739
Size: 1106 895 738
Fragments arranged by size:
1106 895 738
BsaWI w'CCGG_w
Cuts at: 0 19 191 1685 2739
Size: 19 172 1494 1054
Fragments arranged by size:
1494 1054 172 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1091 1121 1378 1408 1636 1666 2404 2434
Size: 1091 30 257 30 228 30 738 30
Cuts at: 2434 2739
Size: 305
Fragments arranged by size:
1091 738 305 257 228 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 187 538 742 886 889 892 1486
Size: 73 114 351 204 144 3 3 594
Cuts at: 1486 1504 1575 2500 2739
Size: 18 71 925 239
Fragments arranged by size:
925 594 351 239 204 144 114 73
71 18 3 3
BseYI C'CCAG_C
Cuts at: 0 1845 2178 2552 2586 2739
Size: 1845 333 374 34 153
Fragments arranged by size:
1845 374 333 153 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1477 1507 1717 1834 1861 2008 2739
Size: 1276 201 30 210 117 27 147 731
Fragments arranged by size:
1276 731 210 201 147 117 30 27
BsiHKAI G_wGCw'C
Cuts at: 0 65 254 418 1415 2456 2739
Size: 65 189 164 997 1041 283
Fragments arranged by size:
1041 997 283 189 164 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2407 2739
Size: 2407 332
Bsp1286I G_dGCh'C
Cuts at: 0 65 254 418 1415 1483 1585 2456 2546
Size: 65 189 164 997 68 102 871 90
Cuts at: 2546 2638 2739
Size: 92 101
Fragments arranged by size:
997 871 189 164 102 101 92 90
68 65
BspEI T'CCGG_A
Cuts at: 0 191 2739
Size: 191 2548
BspHI T'CATG_A
Cuts at: 0 1769 2739
Size: 1769 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1298 1517 2030 2739
Size: 1298 219 513 709
Fragments arranged by size:
1298 709 513 219
BsrBI CCG'CTC
Cuts at: 0 1998 2739
Size: 1998 741
BsrFI r'CCGG_y
Cuts at: 0 19 2739
Size: 19 2720
BssSI C'ACGA_G
Cuts at: 0 1016 2739
Size: 1016 1723
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 864 1190 1915 2076 2235 2409 2739
Size: 237 627 326 725 161 159 174 330
Fragments arranged by size:
725 627 330 326 237 174 161 159
BstYI r'GATC_y
Cuts at: 0 431 1004 1688 1817 2081 2345 2439 2739
Size: 431 573 684 129 264 264 94 300
Fragments arranged by size:
684 573 431 300 264 264 129 94
Bsu36I CC'TnA_GG
Cuts at: 0 1577 2739
Size: 1577 1162
BtgI C'CryG_G
Cuts at: 0 1320 1835 2700 2739
Size: 1320 515 865 39
Fragments arranged by size:
1320 865 515 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1119 2123 2739
Size: 1119 1004 616
Fragments arranged by size:
1119 1004 616
BtsI GCAGTG_nn'
Cuts at: 0 156 1309 2394 2546 2739
Size: 156 1153 1085 152 193
Fragments arranged by size:
1153 1085 193 156 152
DraIII CAC_nnn'GTG
Cuts at: 0 233 1315 2739
Size: 233 1082 1424
Fragments arranged by size:
1424 1082 233
EaeI y'GGCC_r
Cuts at: 0 1917 2093 2576 2739
Size: 1917 176 483 163
Fragments arranged by size:
1917 483 176 163
EarI CTCTTCn'nnn_
Cuts at: 0 236 546 1271 2348 2380 2739
Size: 236 310 725 1077 32 359
Fragments arranged by size:
1077 725 359 310 236 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 932 1852 2739
Size: 932 920 887
Fragments arranged by size:
932 920 887
EcoICRI GAG'CTC
Cuts at: 0 63 252 2739
Size: 63 189 2487
Fragments arranged by size:
2487 189 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1489 2056 2065 2098 2739
Size: 527 962 567 9 33 641
Fragments arranged by size:
962 641 567 527 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 80 868 1099 1876 2739
Size: 80 788 231 777 863
Fragments arranged by size:
863 788 777 231 80
EcoO109I rG'GnC_Cy
Cuts at: 0 1944 2010 2182 2298 2366 2739
Size: 1944 66 172 116 68 373
Fragments arranged by size:
1944 373 172 116 68 66
EcoRV GAT'ATC
Cuts at: 0 1230 2739
Size: 1230 1509
FspI TGC'GCA
Cuts at: 0 1516 1957 2739
Size: 1516 441 782
Fragments arranged by size:
1516 782 441
HaeII r_GCGC'y
Cuts at: 0 864 1464 2004 2739
Size: 864 600 540 735
Fragments arranged by size:
864 735 600 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 25 51 57 442 474 646 678
Size: 19 6 26 6 385 32 172 32
Cuts at: 678 706 738 838 870 1091 1123 1378 1410
Size: 28 32 100 32 221 32 255 32
Cuts at: 1410 1894 1926 2111 2143 2404 2436 2739
Size: 484 32 185 32 261 32 303
Fragments arranged by size:
484 385 303 261 255 221 185 172
100 32 32 32 32 32 32 32
32 32 28 26 19 6 6
KasI G'GCGC_C
Cuts at: 0 2000 2739
Size: 2000 739
KpnI G_GTAC'C
Cuts at: 0 1749 2739
Size: 1749 990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 743 1239 2739
Size: 60 683 496 1500
Fragments arranged by size:
1500 683 496 60
MslI CAynn'nnrTG
Cuts at: 0 1768 1837 2233 2407 2739
Size: 1768 69 396 174 332
Fragments arranged by size:
1768 396 332 174 69
MspA1I CmG'CkG
Cuts at: 0 1084 1566 1845 2151 2574 2702 2739
Size: 1084 482 279 306 423 128 37
Fragments arranged by size:
1084 482 423 306 279 128 37
NarI GG'CG_CC
Cuts at: 0 2001 2739
Size: 2001 738
NcoI C'CATG_G
Cuts at: 0 1835 2739
Size: 1835 904
NdeI CA'TA_TG
Cuts at: 0 267 2739
Size: 267 2472
NspI r_CATG'y
Cuts at: 0 1700 1801 2209 2430 2697 2739
Size: 1700 101 408 221 267 42
Fragments arranged by size:
1700 408 267 221 101 42
PciI A'CATG_T
Cuts at: 0 1696 2205 2426 2739
Size: 1696 509 221 313
Fragments arranged by size:
1696 509 313 221
PfoI T'CCnGG_A
Cuts at: 0 2075 2348 2739
Size: 2075 273 391
Fragments arranged by size:
2075 391 273
PpuMI rG'GwC_Cy
Cuts at: 0 2010 2182 2298 2366 2739
Size: 2010 172 116 68 373
Fragments arranged by size:
2010 373 172 116 68
PstI C_TGCA'G
Cuts at: 0 1967 2573 2739
Size: 1967 606 166
Fragments arranged by size:
1967 606 166
PvuII CAG'CTG
Cuts at: 0 1566 1845 2151 2574 2739
Size: 1566 279 306 423 165
Fragments arranged by size:
1566 423 306 279 165
SacI G_AGCT'C
Cuts at: 0 65 254 2739
Size: 65 189 2485
Fragments arranged by size:
2485 189 65
SacII CC_GC'GG
Cuts at: 0 2703 2739
Size: 2703 36
SanDI GG'GwC_CC
Cuts at: 0 2182 2739
Size: 2182 557
SapI GCTCTTCn'nnn_
Cuts at: 0 546 2739
Size: 546 2193
SbfI CC_TGCA'GG
Cuts at: 0 1967 2739
Size: 1967 772
SexAI A'CCwGG_T
Cuts at: 0 2057 2739
Size: 2057 682
SfcI C'TryA_G
Cuts at: 0 1009 1963 2382 2569 2739
Size: 1009 954 419 187 170
Fragments arranged by size:
1009 954 419 187 170
SfoI GGC'GCC
Cuts at: 0 2002 2739
Size: 2002 737
SmlI C'TyrA_G
Cuts at: 0 759 2739
Size: 759 1980
SphI G_CATG'C
Cuts at: 0 1801 2739
Size: 1801 938
SspI AAT'ATT
Cuts at: 0 1269 2739
Size: 1269 1470
StuI AGG'CCT
Cuts at: 0 1871 2739
Size: 1871 868
StyI C'CwwG_G
Cuts at: 0 155 404 956 1670 1835 1866 1877 1925
Size: 155 249 552 714 165 31 11 48
Cuts at: 1925 2222 2739
Size: 297 517
Fragments arranged by size:
714 552 517 297 249 165 155 48
31 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 988 2227 2739
Size: 795 193 1239 512
Fragments arranged by size:
1239 795 512 193
TatI w'GTAC_w
Cuts at: 0 1658 2739
Size: 1658 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 1636 1668 2739
Size: 1301 32 303 32 1071
Fragments arranged by size:
1301 1071 303 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 568 2320 2418 2739
Size: 568 1752 98 321
Fragments arranged by size:
1752 568 321 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 1842 1932 2739
Size: 285 1557 90 807
Fragments arranged by size:
1557 807 285 90
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbeI BbsI BcgI BclI BglI BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I KasI KpnI MmeI MslI MspA1I NarI NcoI
NdeI NspI PciI PfoI PpuMI PstI PvuII SacI
SacII SanDI SapI SbfI SexAI SfcI SfoI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AclI AflII AloI ApaI AscI AsiSI AvrII
BaeI BbvCI BciVI BfrBI BglII BmgBI BmtI Bpu10I
BsiEI BsiWI BsmI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoRI
FalI FseI FspAI HincII HindIII HpaI MfeI MluI
MscI NaeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XhoI
XmaI XmnI ZraI