(Circular) (Six-base) MAPSORT of: pFN21AE4333.seq Check: 6748 from: 1 to: 5734 pFN21AE4333 5734 bp With 182 enzymes: SgfI * March 28, 2012 13:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 2754 3055 2754 Size: 301 5433 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2923 3797 5181 5449 1632 Size: 743 548 874 1384 268 1917 Fragments arranged by size: 1917 1384 874 743 548 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2603 4633 5610 1203 Size: 1400 2030 977 1327 Fragments arranged by size: 2030 1400 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaI G_GGCC'C Cuts at: 2311 2726 2797 2311 Size: 415 71 5248 Fragments arranged by size: 5248 415 71 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2576 3059 1870 Size: 73 633 483 4545 Fragments arranged by size: 4545 633 483 73 BamHI G'GATC_C Cuts at: 2844 3064 3431 2844 Size: 220 367 5147 Fragments arranged by size: 5147 367 220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 2754 2881 3055 4510 Size: 325 217 627 794 173 127 174 1455 Cuts at: 4510 618 Size: 1842 Fragments arranged by size: 1842 1455 794 627 325 217 174 173 127 BanII G_rGCy'C Cuts at: 1391 2311 2396 2726 2797 3053 1391 Size: 920 85 330 71 256 4072 Fragments arranged by size: 4072 920 330 256 85 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BbvCI CC'TCA_GC Cuts at: 2966 2966 Size: 5734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2140 2174 3925 3959 1903 Size: 34 203 34 1751 34 3678 Fragments arranged by size: 3678 1751 203 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 2589 1548 Size: 1041 4693 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2792 4345 136 Size: 107 122 71 631 605 1120 1553 1525 Fragments arranged by size: 1553 1525 1120 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3023 3151 3023 Size: 128 5606 Bme1580I G_kGCm'C Cuts at: 1790 2311 2726 2797 2886 3795 4951 1790 Size: 521 415 71 89 909 1156 2573 Fragments arranged by size: 2573 1156 909 521 415 89 71 BmgBI CAC'GTC Cuts at: 1318 2604 1318 Size: 1286 4448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2812 2852 4394 1239 Size: 1130 443 40 1542 2579 Fragments arranged by size: 2579 1542 1130 443 40 Bpu10I CC'TnA_GC Cuts at: 2966 2966 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 4397 882 Size: 617 1254 1644 2219 Fragments arranged by size: 2219 1644 1254 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 2337 2555 2628 Size: 53 83 186 936 669 135 218 73 Cuts at: 2628 2700 3541 3923 4558 275 Size: 72 841 382 635 1451 Fragments arranged by size: 1451 936 841 669 635 382 218 186 135 83 73 72 53 BsaWI w'CCGG_w Cuts at: 2076 4167 4565 4839 4986 5397 2076 Size: 2091 398 274 147 411 2413 Fragments arranged by size: 2413 2091 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2312 2342 1178 Size: 30 165 30 909 30 4570 Fragments arranged by size: 4570 909 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2654 2999 1186 Size: 1219 249 345 3921 Fragments arranged by size: 3921 1219 345 249 BseYI C'CCAG_C Cuts at: 2038 2267 2302 2375 2520 2775 2901 2950 4937 Size: 229 35 73 145 255 126 49 1987 Cuts at: 4937 5433 2038 Size: 496 2339 Fragments arranged by size: 2339 1987 496 255 229 145 126 73 49 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2175 2491 1163 Size: 573 439 316 4406 Fragments arranged by size: 4406 573 439 316 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 3184 3945 4973 1540 Size: 275 186 80 1103 761 1028 2301 Fragments arranged by size: 2301 1103 1028 761 275 186 80 BsiHKAI G_wGCw'C Cuts at: 2277 3033 3795 3880 4951 2277 Size: 756 762 85 1071 3060 Fragments arranged by size: 3060 1071 762 756 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 BsmBI CGTCTCn'nnnn_ Cuts at: 2594 2594 Size: 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2311 2396 2726 2797 2886 3033 Size: 399 487 34 85 330 71 89 147 Cuts at: 3033 3053 3795 3880 4951 1391 Size: 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 330 147 89 85 85 71 34 20 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2170 3089 844 Size: 1326 919 3489 Fragments arranged by size: 3489 1326 919 BsrBI CCG'CTC Cuts at: 1394 1823 2093 3621 1394 Size: 429 270 1528 3507 Fragments arranged by size: 3507 1528 429 270 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 4229 4403 65 Size: 1183 1158 1823 174 1396 Fragments arranged by size: 1823 1396 1183 1158 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 4378 4565 1700 Size: 110 352 2216 187 2869 Fragments arranged by size: 2869 2216 352 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 2814 96 Size: 2520 198 3016 Fragments arranged by size: 3016 2520 198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 2225 2874 3794 4806 2225 Size: 649 920 1012 3153 Fragments arranged by size: 3153 1012 920 649 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 4286 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2213 2213 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2844 3064 3431 3816 3833 5285 5371 5383 Size: 872 220 367 385 17 1452 86 12 Cuts at: 5383 5729 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 872 385 367 346 220 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 5734 BtgI C'CryG_G Cuts at: 513 1065 2004 3017 513 Size: 552 939 1013 3230 Fragments arranged by size: 3230 1013 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2243 2257 2503 2564 3250 4044 4064 2158 Size: 85 14 246 61 686 794 20 3828 Fragments arranged by size: 3828 794 686 246 85 61 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2902 2937 529 Size: 35 2338 35 3326 Fragments arranged by size: 3326 2338 35 35 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2342 4741 809 Size: 507 1026 2399 1802 Fragments arranged by size: 2399 1802 1026 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 3181 4070 Size: 54 1425 50 275 18 219 1132 889 Cuts at: 4070 5699 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1132 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3664 1353 Size: 2311 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2375 2812 2852 2923 3797 4394 Size: 393 737 6 437 40 71 874 597 Cuts at: 4394 5181 5449 1239 Size: 787 268 1524 Fragments arranged by size: 1524 874 787 737 597 437 393 268 71 40 6 EcoNI CCTnn'n_nnAGG Cuts at: 2387 2387 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2020 2027 2307 2722 2793 2020 Size: 7 280 415 71 4961 Fragments arranged by size: 4961 415 280 71 7 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 4375 4407 4449 Size: 32 135 32 740 32 2031 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 740 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 2758 3059 2758 Size: 301 5433 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 4848 5032 1305 Size: 1138 2405 184 2007 Fragments arranged by size: 2405 2007 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2051 10 Size: 54 1425 343 219 3693 Fragments arranged by size: 3693 1425 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 3692 4051 4210 518 Size: 648 1045 1481 359 159 2042 Fragments arranged by size: 2042 1481 1045 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2252 3150 3827 4975 5220 1606 Size: 432 214 898 677 1148 245 2120 Fragments arranged by size: 2120 1148 898 677 432 245 214 NcoI C'CATG_G Cuts at: 513 1065 2004 3017 513 Size: 552 939 1013 3230 Fragments arranged by size: 3230 1013 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 2398 2956 1348 Size: 1050 558 4126 Fragments arranged by size: 4126 1050 558 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PfoI T'CCnGG_A Cuts at: 2385 2840 2385 Size: 455 5279 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2020 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2307 2722 2793 2307 Size: 415 71 5248 Fragments arranged by size: 5248 415 71 PspXI vC'TCGA_Gb Cuts at: 2576 2576 Size: 5734 PstI C_TGCA'G Cuts at: 830 2476 2571 3086 830 Size: 1646 95 515 3478 Fragments arranged by size: 3478 1646 515 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2038 2252 2038 Size: 214 5520 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2472 2567 3082 4217 4898 5089 826 Size: 219 1427 95 515 1135 681 191 1471 Fragments arranged by size: 1471 1427 1135 681 515 219 191 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 3838 4739 5001 820 Size: 197 926 633 1262 901 262 1553 Fragments arranged by size: 1553 1262 926 901 633 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 2806 1517 Size: 1289 4445 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3017 3173 513 Size: 552 939 336 677 156 3074 Fragments arranged by size: 3074 939 677 552 336 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 3761 3778 3931 4116 4561 325 Size: 1255 650 1531 17 153 185 445 1498 Fragments arranged by size: 1531 1498 1255 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 2814 3980 Size: 274 80 33 51 1443 639 198 1166 Cuts at: 3980 5692 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1166 639 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2654 2686 5694 5726 2654 Size: 32 3008 32 2662 Fragments arranged by size: 3008 2662 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2213 2714 2213 Size: 501 5233 XhoI C'TCGA_G Cuts at: 1943 2576 1943 Size: 633 5101 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI