(Linear) (Six-base) MAPSORT of: KIEE4333.seq Check: 9367 from: 1 to: 1029 KIEE4333.orf With 182 enzymes: SgfI * March 22, 2012 17:45 .. Acc65I G'GTAC_C Cuts at: 0 749 1029 Size: 749 280 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 918 1029 Size: 370 548 111 Fragments arranged by size: 548 370 111 AflIII A'CryG_T Cuts at: 0 598 1029 Size: 598 431 ApaI G_GGCC'C Cuts at: 0 306 721 792 1029 Size: 306 415 71 237 Fragments arranged by size: 415 306 237 71 AvaI C'yCGr_G Cuts at: 0 571 1029 Size: 571 458 BamHI G'GATC_C Cuts at: 0 839 1029 Size: 839 190 BanI G'GyrC_C Cuts at: 0 576 749 876 1029 Size: 576 173 127 153 Fragments arranged by size: 576 173 153 127 BanII G_rGCy'C Cuts at: 0 306 391 721 792 1029 Size: 306 85 330 71 237 Fragments arranged by size: 330 306 237 85 71 BbvCI CC'TCA_GC Cuts at: 0 961 1029 Size: 961 68 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 135 169 1029 Size: 135 34 860 Fragments arranged by size: 860 135 34 BclI T'GATC_A Cuts at: 0 584 1029 Size: 584 445 BglI GCCn_nnn'nGGC Cuts at: 0 787 1029 Size: 787 242 BlpI GC'TnA_GC Cuts at: 0 1018 1029 Size: 1018 11 Bme1580I G_kGCm'C Cuts at: 0 306 721 792 881 1029 Size: 306 415 71 89 148 Fragments arranged by size: 415 306 148 89 71 BmgBI CAC'GTC Cuts at: 0 599 1029 Size: 599 430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 807 847 1029 Size: 364 443 40 182 Fragments arranged by size: 443 364 182 40 Bpu10I CC'TnA_GC Cuts at: 0 961 1029 Size: 961 68 BsaI GGTCTCn'nnnn_ Cuts at: 0 748 1029 Size: 748 281 BsaHI Gr'CG_yC Cuts at: 0 197 332 550 623 695 1029 Size: 197 135 218 73 72 334 Fragments arranged by size: 334 218 197 135 73 72 BsaWI w'CCGG_w Cuts at: 0 71 1029 Size: 71 958 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 307 337 1029 Size: 307 30 692 Fragments arranged by size: 692 307 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 400 649 994 1029 Size: 400 249 345 35 Fragments arranged by size: 400 345 249 35 BseYI C'CCAG_C Cuts at: 0 33 262 297 370 515 770 896 945 Size: 33 229 35 73 145 255 126 49 Cuts at: 945 1029 Size: 84 Fragments arranged by size: 255 229 145 126 84 73 49 35 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 486 1029 Size: 170 316 543 Fragments arranged by size: 543 316 170 BsiEI CG_ry'CG Cuts at: 0 76 1029 Size: 76 953 BsiHKAI G_wGCw'C Cuts at: 0 272 1028 1029 Size: 272 756 1 Fragments arranged by size: 756 272 1 BsmBI CGTCTCn'nnnn_ Cuts at: 0 589 1029 Size: 589 440 Bsp1286I G_dGCh'C Cuts at: 0 272 306 391 721 792 881 1028 1029 Size: 272 34 85 330 71 89 147 1 Fragments arranged by size: 330 272 147 89 85 71 34 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 165 1029 Size: 165 864 BsrBI CCG'CTC Cuts at: 0 88 1029 Size: 88 941 BsrDI GCAATG_nn' Cuts at: 0 401 1029 Size: 401 628 BsrFI r'CCGG_y Cuts at: 0 157 1029 Size: 157 872 BsrGI T'GTAC_A Cuts at: 0 611 809 1029 Size: 611 198 220 Fragments arranged by size: 611 220 198 BssSI C'ACGA_G Cuts at: 0 220 869 1029 Size: 220 649 160 Fragments arranged by size: 649 220 160 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 1029 Size: 237 792 BstXI CCAn_nnnn'nTGG Cuts at: 0 208 1029 Size: 208 821 BstYI r'GATC_y Cuts at: 0 839 1029 Size: 839 190 Bsu36I CC'TnA_GG Cuts at: 0 632 1029 Size: 632 397 BtgI C'CryG_G Cuts at: 0 1012 1029 Size: 1012 17 BtsI GCAGTG_nn' Cuts at: 0 153 238 252 498 559 1029 Size: 153 85 14 246 61 470 Fragments arranged by size: 470 246 153 85 61 14 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 897 932 1029 Size: 897 35 97 Fragments arranged by size: 897 97 35 DrdI GACnn_nn'nnGTC Cuts at: 0 337 1029 Size: 337 692 EaeI y'GGCC_r Cuts at: 0 44 1029 Size: 44 985 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 370 807 847 918 1029 Size: 364 6 437 40 71 111 Fragments arranged by size: 437 364 111 71 40 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 382 1029 Size: 382 647 EcoO109I rG'GnC_Cy Cuts at: 0 15 22 302 717 788 1029 Size: 15 7 280 415 71 241 Fragments arranged by size: 415 280 241 71 15 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 307 339 1029 Size: 307 32 690 Fragments arranged by size: 690 307 32 KpnI G_GTAC'C Cuts at: 0 753 1029 Size: 753 276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 438 1029 Size: 438 591 MscI TGG'CCA Cuts at: 0 46 1029 Size: 46 983 MslI CAynn'nnrTG Cuts at: 0 206 1029 Size: 206 823 MspA1I CmG'CkG Cuts at: 0 33 247 1029 Size: 33 214 782 Fragments arranged by size: 782 214 33 NcoI C'CATG_G Cuts at: 0 1012 1029 Size: 1012 17 PasI CC'CwG_GG Cuts at: 0 393 951 1029 Size: 393 558 78 Fragments arranged by size: 558 393 78 PfoI T'CCnGG_A Cuts at: 0 380 835 1029 Size: 380 455 194 Fragments arranged by size: 455 380 194 PpuMI rG'GwC_Cy Cuts at: 0 15 1029 Size: 15 1014 PspOMI G'GGCC_C Cuts at: 0 302 717 788 1029 Size: 302 415 71 241 Fragments arranged by size: 415 302 241 71 PspXI vC'TCGA_Gb Cuts at: 0 571 1029 Size: 571 458 PstI C_TGCA'G Cuts at: 0 471 566 1029 Size: 471 95 463 Fragments arranged by size: 471 463 95 PvuII CAG'CTG Cuts at: 0 33 247 1029 Size: 33 214 782 Fragments arranged by size: 782 214 33 SfcI C'TryA_G Cuts at: 0 467 562 1029 Size: 467 95 467 Fragments arranged by size: 467 467 95 SmlI C'TyrA_G Cuts at: 0 571 1029 Size: 571 458 StuI AGG'CCT Cuts at: 0 801 1029 Size: 801 228 StyI C'CwwG_G Cuts at: 0 335 1012 1029 Size: 335 677 17 Fragments arranged by size: 677 335 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 225 1029 Size: 225 804 TatI w'GTAC_w Cuts at: 0 611 809 1029 Size: 611 198 220 Fragments arranged by size: 611 220 198 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 649 681 1029 Size: 649 32 348 Fragments arranged by size: 649 348 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 208 709 1029 Size: 208 501 320 Fragments arranged by size: 501 320 208 XhoI C'TCGA_G Cuts at: 0 571 1029 Size: 571 458 Enzymes that do cut: Acc65I AcuI AflIII ApaI AvaI BamHI BanI BanII BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI MscI MslI MspA1I NcoI PasI PfoI PpuMI PspOMI PspXI PstI PvuII SfcI SmlI StuI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BfrBI BglII BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI ZraI