(Circular) (Six-base) MAPSORT of: pFN21AE4571.seq Check: 5757 from: 1 to: 6916 pFN21AE4571 6916 bp With 182 enzymes: SgfI * March 28, 2012 14:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3331 3331 Size: 6916 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4726 278 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4259 1937 Size: 2322 4594 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 5043 5416 1563 Size: 3480 373 3063 Fragments arranged by size: 3480 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2268 2726 3086 3863 3908 4979 6363 Size: 506 130 458 360 777 45 1071 1384 Cuts at: 6363 6631 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1071 777 506 458 360 268 130 45 AfeI AGC'GCT Cuts at: 3863 3863 Size: 6916 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6719 AflIII A'CryG_T Cuts at: 1203 3222 5815 6792 1203 Size: 2019 2593 977 1327 Fragments arranged by size: 2593 2019 1327 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2253 1184 Size: 1069 5847 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2367 2399 2441 2473 3482 3514 2367 Size: 32 42 32 1009 32 5769 Fragments arranged by size: 5769 1009 42 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2836 3064 3886 4063 4085 4659 6231 2836 Size: 228 822 177 22 574 1572 3521 Fragments arranged by size: 3521 1572 822 574 228 177 22 ApaI G_GGCC'C Cuts at: 3327 3922 4190 3327 Size: 595 268 6053 Fragments arranged by size: 6053 595 268 ApaLI G'TGCA_C Cuts at: 4973 6129 4973 Size: 1156 5760 ApoI r'AATT_y Cuts at: 1493 2717 2984 4225 4451 4603 6712 1493 Size: 1224 267 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 1224 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 5470 160 Size: 872 4438 1606 Fragments arranged by size: 4438 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 2745 2976 3807 3959 4130 4241 1870 Size: 73 802 231 831 152 171 111 4545 Fragments arranged by size: 4545 831 802 231 171 152 111 73 AvrII C'CTAG_G Cuts at: 2386 2386 Size: 6916 BamHI G'GATC_C Cuts at: 2904 4246 4613 2904 Size: 1342 367 5207 Fragments arranged by size: 5207 1342 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3795 4237 5692 618 Size: 325 217 627 2008 442 1455 1842 Fragments arranged by size: 2008 1842 1455 627 442 325 217 BanII G_rGCy'C Cuts at: 1391 2870 2894 3327 3922 4190 4235 1391 Size: 1479 24 433 595 268 45 4072 Fragments arranged by size: 4072 1479 595 433 268 45 24 BbeI G_GCGC'C Cuts at: 3799 3799 Size: 6916 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5945 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3367 3401 3917 3951 5107 5141 1903 Size: 34 1430 34 516 34 1156 34 3678 Fragments arranged by size: 3678 1430 1156 516 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4808 6018 4808 Size: 1210 5706 BclI T'GATC_A Cuts at: 1548 3432 1548 Size: 1884 5032 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2552 4044 5527 Size: 107 122 71 631 605 880 1492 1483 Cuts at: 5527 136 Size: 1525 Fragments arranged by size: 1525 1492 1483 880 631 605 122 107 71 BglII A'GATC_T Cuts at: 3582 6911 3582 Size: 3329 3587 BlpI GC'TnA_GC Cuts at: 2192 2403 4333 2192 Size: 211 1930 4775 Fragments arranged by size: 4775 1930 211 Bme1580I G_kGCm'C Cuts at: 1790 3327 3922 4190 4977 6133 1790 Size: 1537 595 268 787 1156 2573 Fragments arranged by size: 2573 1537 1156 787 595 268 BmgBI CAC'GTC Cuts at: 1318 3616 4054 1318 Size: 2298 438 4180 Fragments arranged by size: 4180 2298 438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2011 2501 2836 Size: 416 53 83 399 24 594 490 335 Cuts at: 2836 4185 4647 5605 442 Size: 1349 462 958 1753 Fragments arranged by size: 1753 1349 958 594 490 462 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 2753 2810 3305 3499 3599 3686 3764 Size: 1247 267 57 495 194 100 87 78 Cuts at: 3764 5576 1239 Size: 1812 2579 Fragments arranged by size: 2579 1812 1247 495 267 194 100 87 78 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5041 5906 6204 5041 Size: 865 298 5753 Fragments arranged by size: 5753 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3476 3554 5579 882 Size: 617 1977 78 2025 2219 Fragments arranged by size: 2219 2025 1977 617 78 BsaAI yAC'GTr Cuts at: 493 1204 3625 4707 493 Size: 711 2421 1082 2702 Fragments arranged by size: 2702 2421 1082 711 BsaBI GATnn'nnATC Cuts at: 6910 6910 Size: 6916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4076 4723 5105 Size: 53 83 186 936 2263 280 647 382 Cuts at: 5105 5740 275 Size: 635 1451 Fragments arranged by size: 2263 1451 936 647 635 382 280 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 5349 5747 6021 6168 6579 3834 Size: 1515 398 274 147 411 4171 Fragments arranged by size: 4171 1515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 3110 3140 3757 Size: 30 165 30 1038 30 639 30 617 Cuts at: 3757 3787 1178 Size: 30 4307 Fragments arranged by size: 4307 1038 639 617 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2279 2994 3302 4154 1186 Size: 1090 3 715 308 852 3948 Fragments arranged by size: 3948 1090 852 715 308 3 BseYI C'CCAG_C Cuts at: 3229 3361 6119 6615 3229 Size: 132 2758 496 3530 Fragments arranged by size: 3530 2758 496 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 3335 3359 4076 1163 Size: 573 477 1122 24 717 4003 Fragments arranged by size: 4003 1122 717 573 477 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4366 5127 6155 1540 Size: 275 186 1822 543 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 543 275 186 BsiHKAI G_wGCw'C Cuts at: 4977 5062 6133 4977 Size: 85 1071 5760 Fragments arranged by size: 5760 1071 85 BsmI GAATG_Cn' Cuts at: 3407 4427 4520 3407 Size: 1020 93 5803 Fragments arranged by size: 5803 1020 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3437 3437 Size: 6916 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2870 2894 3327 3922 4190 4235 4977 Size: 399 1080 24 433 595 268 45 742 Cuts at: 4977 5062 6133 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1080 1071 742 595 433 399 268 85 45 24 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 4805 6535 4805 Size: 1730 5186 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2450 3331 4271 844 Size: 1606 881 940 3489 Fragments arranged by size: 3489 1606 940 881 BsrBI CCG'CTC Cuts at: 1394 1823 3037 3477 4803 1394 Size: 429 1214 440 1326 3507 Fragments arranged by size: 3507 1326 1214 440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5411 5585 65 Size: 1183 4163 174 1396 Fragments arranged by size: 4163 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3167 5560 5747 1700 Size: 110 1357 2393 187 2869 Fragments arranged by size: 2869 2393 1357 187 110 BsrGI T'GTAC_A Cuts at: 96 3306 96 Size: 3210 3706 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6916 BssSI C'ACGA_G Cuts at: 3353 4976 5988 3353 Size: 1623 1012 4281 Fragments arranged by size: 4281 1623 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3358 794 Size: 2564 4352 BstBI TT'CG_AA Cuts at: 5720 5720 Size: 6916 BstEII G'GTnAC_C Cuts at: 1184 2740 1184 Size: 1556 5360 BstXI CCAn_nnnn'nTGG Cuts at: 3374 3374 Size: 6916 BstYI r'GATC_y Cuts at: 1972 2904 3582 3831 4143 4246 4613 4998 5015 Size: 932 678 249 312 103 367 385 17 Cuts at: 5015 6467 6553 6565 6911 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 932 678 385 367 346 312 249 103 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2166 3681 3977 2166 Size: 1515 296 5105 Fragments arranged by size: 5105 1515 296 BtgI C'CryG_G Cuts at: 513 1065 2004 2292 2535 3367 513 Size: 552 939 288 243 832 4062 Fragments arranged by size: 4062 939 832 552 288 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3493 487 Size: 683 56 2267 3910 Fragments arranged by size: 3910 2267 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4432 5226 5246 3379 Size: 1053 794 20 5049 Fragments arranged by size: 5049 1053 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6916 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6881 DraI TTT'AAA Cuts at: 4220 4220 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5923 809 Size: 507 4607 1802 Fragments arranged by size: 4607 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3906 4158 4207 Size: 54 1425 50 275 18 2076 252 49 Cuts at: 4207 4363 5252 6881 8 Size: 156 889 1629 43 Fragments arranged by size: 2076 1629 1425 889 275 252 156 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4363 1537 Size: 275 2551 4090 Fragments arranged by size: 4090 2551 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2609 2702 3389 3519 4846 1353 Size: 854 402 93 687 130 1327 3423 Fragments arranged by size: 3423 1327 854 687 402 130 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2063 3803 5503 5877 6023 Size: 1064 138 435 39 1740 1700 374 146 Cuts at: 6023 387 Size: 1280 Fragments arranged by size: 1740 1700 1280 1064 435 374 146 138 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2268 2486 2726 2753 2810 3086 Size: 393 506 130 218 240 27 57 276 Cuts at: 3086 3305 3499 3599 3686 3764 3863 3908 4979 Size: 219 194 100 87 78 99 45 1071 Cuts at: 4979 5576 6363 6631 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1071 787 597 506 393 276 268 240 219 218 194 130 100 99 87 78 57 45 27 EcoNI CCTnn'n_nnAGG Cuts at: 2258 2771 3317 3926 2258 Size: 513 546 609 5248 Fragments arranged by size: 5248 609 546 513 EcoO109I rG'GnC_Cy Cuts at: 2170 2215 2428 2918 3301 3323 3324 3919 4186 Size: 45 213 490 383 22 1 595 267 Cuts at: 4186 4187 4198 2170 Size: 1 11 4888 Fragments arranged by size: 4888 595 490 383 267 213 45 22 11 1 1 EcoRI G'AATT_C Cuts at: 2717 4225 4603 2717 Size: 1508 378 5030 Fragments arranged by size: 5030 1508 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3295 3295 Size: 6916 FspI TGC'GCA Cuts at: 5422 5422 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 2098 3799 3865 6063 1678 Size: 420 1701 66 2198 2531 Fragments arranged by size: 2531 2198 1701 420 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3110 3142 3454 3486 3532 Size: 32 135 32 1538 32 312 32 46 Cuts at: 3532 3564 4187 4219 5557 5589 5631 5663 1373 Size: 32 623 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1538 1338 623 312 135 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4260 4506 669 Size: 988 281 2322 246 3079 Fragments arranged by size: 3079 2322 988 281 246 HindIII A'AGCT_T Cuts at: 748 3272 748 Size: 2524 4392 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KasI G'GCGC_C Cuts at: 3795 3795 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4515 4515 Size: 6916 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 4116 6030 6214 1305 Size: 1026 1785 1914 184 2007 Fragments arranged by size: 2007 1914 1785 1026 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4160 4209 10 Size: 54 1425 343 2328 49 2717 Fragments arranged by size: 2717 2328 1425 343 54 49 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2235 3372 4874 5233 5392 518 Size: 648 876 193 1137 1502 359 159 2042 Fragments arranged by size: 2042 1502 1137 876 648 359 193 159 MspA1I CmG'CkG Cuts at: 1606 2227 2294 2788 3061 3265 4082 4156 4332 Size: 621 67 494 273 204 817 74 176 Cuts at: 4332 5009 6157 6402 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 817 677 621 494 273 245 204 176 74 67 NaeI GCC'GGC Cuts at: 3169 3169 Size: 6916 NarI GG'CG_CC Cuts at: 3796 3796 Size: 6916 NcoI C'CATG_G Cuts at: 513 1065 2004 2535 3367 513 Size: 552 939 531 832 4062 Fragments arranged by size: 4062 939 832 552 531 NdeI CA'TA_TG Cuts at: 387 3708 387 Size: 3321 3595 NgoMIV G'CCGG_C Cuts at: 3167 3167 Size: 6916 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2240 4274 5819 1132 Size: 97 1011 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 1011 97 PasI CC'CwG_GG Cuts at: 1348 2863 1348 Size: 1515 5401 PciI A'CATG_T Cuts at: 5815 5815 Size: 6916 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 4124 1108 Size: 1668 1348 3900 Fragments arranged by size: 3900 1668 1348 PfoI T'CCnGG_A Cuts at: 2907 2907 Size: 6916 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2441 2473 4985 5017 6498 6530 2441 Size: 32 2512 32 1481 32 2827 Fragments arranged by size: 2827 2512 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 3301 2215 Size: 1086 5830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PspOMI G'GGCC_C Cuts at: 3323 3918 4186 3323 Size: 595 268 6053 Fragments arranged by size: 6053 595 268 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2261 2293 2261 Size: 32 6884 PstI C_TGCA'G Cuts at: 830 4268 830 Size: 3438 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 2227 2788 3061 4082 4156 2227 Size: 561 273 1021 74 4987 Fragments arranged by size: 4987 1021 561 273 74 SacII CC_GC'GG Cuts at: 2295 2295 Size: 6916 SalI G'TCGA_C Cuts at: 1936 4258 1936 Size: 2322 4594 SapI GCTCTTCn'nnn_ Cuts at: 2609 2702 3389 2609 Size: 93 687 6136 Fragments arranged by size: 6136 687 93 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 5164 6876 5164 Size: 1712 5204 SfcI C'TryA_G Cuts at: 826 1045 4264 5399 6080 6271 826 Size: 219 3219 1135 681 191 1471 Fragments arranged by size: 3219 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3797 3797 Size: 6916 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 2747 3809 4243 1872 Size: 875 1062 434 4545 Fragments arranged by size: 4545 1062 875 434 SmlI C'TyrA_G Cuts at: 820 1017 1943 5020 5921 6183 820 Size: 197 926 3077 901 262 1553 Fragments arranged by size: 3077 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 4274 1132 Size: 3142 3774 SspI AAT'ATT Cuts at: 5 52 4840 5 Size: 47 4788 2081 Fragments arranged by size: 4788 2081 47 StuI AGG'CCT Cuts at: 1517 2069 2139 2253 2804 3686 1517 Size: 552 70 114 551 882 4747 Fragments arranged by size: 4747 882 552 551 114 70 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2386 2471 2535 2799 3367 Size: 552 939 357 25 85 64 264 568 Cuts at: 3367 4355 513 Size: 988 3074 Fragments arranged by size: 3074 988 939 568 552 357 264 85 64 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2101 2427 2876 4943 4960 5113 Size: 1255 450 71 326 449 2067 17 153 Cuts at: 5113 5298 5743 325 Size: 185 445 1498 Fragments arranged by size: 2067 1498 1255 450 449 445 326 185 153 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 3306 5162 Size: 274 80 33 51 1443 42 1287 1856 Cuts at: 5162 6874 96 Size: 1712 138 Fragments arranged by size: 1856 1712 1443 1287 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6876 6908 6876 Size: 32 6884 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6916 XbaI T'CTAG_A Cuts at: 3856 4252 3856 Size: 396 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 3901 2368 Size: 1533 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6916 XmaI C'CCGG_G Cuts at: 1870 2745 3807 4241 1870 Size: 875 1062 434 4545 Fragments arranged by size: 4545 1062 875 434 XmnI GAAnn'nnTTC Cuts at: 5045 5045 Size: 6916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4724 276 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SanDI SexAI SfiI SrfI SwaI