(Linear) (Six-base) MAPSORT of: KIEE4571.seq Check: 4081 from: 1 to: 2211 KIEE4571.orf With 182 enzymes: SgfI * March 22, 2012 18:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1326 2211 Size: 1326 885 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 263 721 1081 1858 1903 2211 Size: 133 130 458 360 777 45 308 Fragments arranged by size: 777 458 360 308 133 130 45 AfeI AGC'GCT Cuts at: 0 1858 2211 Size: 1858 353 AflIII A'CryG_T Cuts at: 0 1217 2211 Size: 1217 994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 248 2211 Size: 248 1963 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 362 394 436 468 1477 1509 2211 Size: 362 32 42 32 1009 32 702 Fragments arranged by size: 1009 702 362 42 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 831 1059 1881 2058 2080 2211 Size: 831 228 822 177 22 131 Fragments arranged by size: 831 822 228 177 131 22 ApaI G_GGCC'C Cuts at: 0 1322 1917 2185 2211 Size: 1322 595 268 26 Fragments arranged by size: 1322 595 268 26 ApoI r'AATT_y Cuts at: 0 712 979 2211 Size: 712 267 1232 Fragments arranged by size: 1232 712 267 AvaI C'yCGr_G Cuts at: 0 740 971 1802 1954 2125 2211 Size: 740 231 831 152 171 86 Fragments arranged by size: 831 740 231 171 152 86 AvrII C'CTAG_G Cuts at: 0 381 2211 Size: 381 1830 BamHI G'GATC_C Cuts at: 0 899 2211 Size: 899 1312 BanI G'GyrC_C Cuts at: 0 1790 2211 Size: 1790 421 BanII G_rGCy'C Cuts at: 0 865 889 1322 1917 2185 2211 Size: 865 24 433 595 268 26 Fragments arranged by size: 865 595 433 268 26 24 BbeI G_GCGC'C Cuts at: 0 1794 2211 Size: 1794 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1362 1396 1912 1946 2211 Size: 1362 34 516 34 265 Fragments arranged by size: 1362 516 265 34 34 BclI T'GATC_A Cuts at: 0 1427 2211 Size: 1427 784 BglI GCCn_nnn'nGGC Cuts at: 0 547 2039 2211 Size: 547 1492 172 Fragments arranged by size: 1492 547 172 BglII A'GATC_T Cuts at: 0 1577 2211 Size: 1577 634 BlpI GC'TnA_GC Cuts at: 0 187 398 2211 Size: 187 211 1813 Fragments arranged by size: 1813 211 187 Bme1580I G_kGCm'C Cuts at: 0 1322 1917 2185 2211 Size: 1322 595 268 26 Fragments arranged by size: 1322 595 268 26 BmgBI CAC'GTC Cuts at: 0 1611 2049 2211 Size: 1611 438 162 Fragments arranged by size: 1611 438 162 BmrI ACTGGGnnnn_n' Cuts at: 0 6 496 831 2180 2211 Size: 6 490 335 1349 31 Fragments arranged by size: 1349 490 335 31 6 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 748 805 1300 1494 1594 1681 1759 Size: 481 267 57 495 194 100 87 78 Cuts at: 1759 2211 Size: 452 Fragments arranged by size: 495 481 452 267 194 100 87 78 57 BsaI GGTCTCn'nnnn_ Cuts at: 0 1471 1549 2211 Size: 1471 78 662 Fragments arranged by size: 1471 662 78 BsaAI yAC'GTr Cuts at: 0 1620 2211 Size: 1620 591 BsaHI Gr'CG_yC Cuts at: 0 1791 2071 2211 Size: 1791 280 140 Fragments arranged by size: 1791 280 140 BsaWI w'CCGG_w Cuts at: 0 1829 2211 Size: 1829 382 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 436 466 1105 1135 1752 1782 2211 Size: 436 30 639 30 617 30 429 Fragments arranged by size: 639 617 436 429 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 274 989 1297 2149 2211 Size: 271 3 715 308 852 62 Fragments arranged by size: 852 715 308 271 62 3 BseYI C'CCAG_C Cuts at: 0 1224 1356 2211 Size: 1224 132 855 Fragments arranged by size: 1224 855 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 1330 1354 2071 2211 Size: 208 1122 24 717 140 Fragments arranged by size: 1122 717 208 140 24 BsiEI CG_ry'CG Cuts at: 0 1818 2211 Size: 1818 393 BsmI GAATG_Cn' Cuts at: 0 1402 2211 Size: 1402 809 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1432 2211 Size: 1432 779 Bsp1286I G_dGCh'C Cuts at: 0 865 889 1322 1917 2185 2211 Size: 865 24 433 595 268 26 Fragments arranged by size: 865 595 433 268 26 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 445 1326 2211 Size: 445 881 885 Fragments arranged by size: 885 881 445 BsrBI CCG'CTC Cuts at: 0 1032 1472 2211 Size: 1032 440 739 Fragments arranged by size: 1032 739 440 BsrFI r'CCGG_y Cuts at: 0 1162 2211 Size: 1162 1049 BsrGI T'GTAC_A Cuts at: 0 1301 2211 Size: 1301 910 BssSI C'ACGA_G Cuts at: 0 1348 2211 Size: 1348 863 BstAPI GCAn_nnn'nTGC Cuts at: 0 1353 2211 Size: 1353 858 BstEII G'GTnAC_C Cuts at: 0 735 2211 Size: 735 1476 BstXI CCAn_nnnn'nTGG Cuts at: 0 1369 2211 Size: 1369 842 BstYI r'GATC_y Cuts at: 0 899 1577 1826 2138 2211 Size: 899 678 249 312 73 Fragments arranged by size: 899 678 312 249 73 Bsu36I CC'TnA_GG Cuts at: 0 161 1676 1972 2211 Size: 161 1515 296 239 Fragments arranged by size: 1515 296 239 161 BtgI C'CryG_G Cuts at: 0 287 530 1362 2211 Size: 287 243 832 849 Fragments arranged by size: 849 832 287 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1488 2211 Size: 1488 723 BtsI GCAGTG_nn' Cuts at: 0 1374 2211 Size: 1374 837 EaeI y'GGCC_r Cuts at: 0 1901 2153 2202 2211 Size: 1901 252 49 9 Fragments arranged by size: 1901 252 49 9 EarI CTCTTCn'nnn_ Cuts at: 0 202 604 697 1384 1514 2211 Size: 202 402 93 687 130 697 Fragments arranged by size: 697 687 402 202 130 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 58 1798 2211 Size: 19 39 1740 413 Fragments arranged by size: 1740 413 39 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 263 481 721 748 805 1081 1300 Size: 133 130 218 240 27 57 276 219 Cuts at: 1300 1494 1594 1681 1759 1858 1903 2211 Size: 194 100 87 78 99 45 308 Fragments arranged by size: 308 276 240 219 218 194 133 130 100 99 87 78 57 45 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 253 766 1312 1921 2211 Size: 253 513 546 609 290 Fragments arranged by size: 609 546 513 290 253 EcoO109I rG'GnC_Cy Cuts at: 0 165 210 423 913 1296 1318 1319 1914 Size: 165 45 213 490 383 22 1 595 Cuts at: 1914 2181 2182 2193 2211 Size: 267 1 11 18 Fragments arranged by size: 595 490 383 267 213 165 45 22 18 11 1 1 EcoRI G'AATT_C Cuts at: 0 712 2211 Size: 712 1499 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1290 2211 Size: 1290 921 HaeII r_GCGC'y Cuts at: 0 93 1794 1860 2211 Size: 93 1701 66 351 Fragments arranged by size: 1701 351 93 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1105 1137 1449 1481 1527 1559 2182 2211 Size: 1105 32 312 32 46 32 623 29 Fragments arranged by size: 1105 623 312 46 32 32 32 29 HindIII A'AGCT_T Cuts at: 0 1267 2211 Size: 1267 944 KasI G'GCGC_C Cuts at: 0 1790 2211 Size: 1790 421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 326 2111 2211 Size: 326 1785 100 Fragments arranged by size: 1785 326 100 MscI TGG'CCA Cuts at: 0 2155 2204 2211 Size: 2155 49 7 Fragments arranged by size: 2155 49 7 MslI CAynn'nnrTG Cuts at: 0 37 230 1367 2211 Size: 37 193 1137 844 Fragments arranged by size: 1137 844 193 37 MspA1I CmG'CkG Cuts at: 0 222 289 783 1056 1260 2077 2151 2211 Size: 222 67 494 273 204 817 74 60 Fragments arranged by size: 817 494 273 222 204 74 67 60 NaeI GCC'GGC Cuts at: 0 1164 2211 Size: 1164 1047 NarI GG'CG_CC Cuts at: 0 1791 2211 Size: 1791 420 NcoI C'CATG_G Cuts at: 0 530 1362 2211 Size: 530 832 849 Fragments arranged by size: 849 832 530 NdeI CA'TA_TG Cuts at: 0 1703 2211 Size: 1703 508 NgoMIV G'CCGG_C Cuts at: 0 1162 2211 Size: 1162 1049 NspI r_CATG'y Cuts at: 0 235 2211 Size: 235 1976 PasI CC'CwG_GG Cuts at: 0 858 2211 Size: 858 1353 PflMI CCAn_nnn'nTGG Cuts at: 0 771 2119 2211 Size: 771 1348 92 Fragments arranged by size: 1348 771 92 PfoI T'CCnGG_A Cuts at: 0 902 2211 Size: 902 1309 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 436 468 2211 Size: 436 32 1743 Fragments arranged by size: 1743 436 32 PpuMI rG'GwC_Cy Cuts at: 0 210 1296 2211 Size: 210 1086 915 Fragments arranged by size: 1086 915 210 PspOMI G'GGCC_C Cuts at: 0 1318 1913 2181 2211 Size: 1318 595 268 30 Fragments arranged by size: 1318 595 268 30 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 256 288 2211 Size: 256 32 1923 Fragments arranged by size: 1923 256 32 PvuII CAG'CTG Cuts at: 0 222 783 1056 2077 2151 2211 Size: 222 561 273 1021 74 60 Fragments arranged by size: 1021 561 273 222 74 60 SacII CC_GC'GG Cuts at: 0 290 2211 Size: 290 1921 SapI GCTCTTCn'nnn_ Cuts at: 0 604 697 1384 2211 Size: 604 93 687 827 Fragments arranged by size: 827 687 604 93 SfoI GGC'GCC Cuts at: 0 1792 2211 Size: 1792 419 SmaI CCC'GGG Cuts at: 0 742 1804 2211 Size: 742 1062 407 Fragments arranged by size: 1062 742 407 StuI AGG'CCT Cuts at: 0 64 134 248 799 1681 2211 Size: 64 70 114 551 882 530 Fragments arranged by size: 882 551 530 114 70 64 StyI C'CwwG_G Cuts at: 0 356 381 466 530 794 1362 2211 Size: 356 25 85 64 264 568 849 Fragments arranged by size: 849 568 356 264 85 64 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 96 422 871 2211 Size: 25 71 326 449 1340 Fragments arranged by size: 1340 449 326 71 25 TatI w'GTAC_w Cuts at: 0 14 1301 2211 Size: 14 1287 910 Fragments arranged by size: 1287 910 14 XbaI T'CTAG_A Cuts at: 0 1851 2211 Size: 1851 360 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 363 1896 2211 Size: 363 1533 315 Fragments arranged by size: 1533 363 315 XmaI C'CCGG_G Cuts at: 0 740 1802 2211 Size: 740 1062 409 Fragments arranged by size: 1062 740 409 Enzymes that do cut: AarI AcuI AfeI AflIII AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI PvuII SacII SapI SfoI SmaI StuI StyI TaqII TatI XbaI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI ApaLI AscI AseI AsiSI BaeI BbsI BbvCI BciVI BfrBI BmtI BplI Bpu10I BpuEI BsaBI BsiHKAI BsiWI BspEI BspHI BsrDI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI XmnI ZraI