################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AcsI 350 GAATTC [GA]^AATT[CT] AcyI 1237 GGCGCC G[GA]^CG[CT]C AlwNI 913 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 68 CCTCAGG CC^T[ACGT]AGG ApaI 890 GGGCCC GGGCC^C ApoI 350 GAATTC [GA]^AATT[CT] Asp700I 546 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 591 GAGCTC G[AT]GC[AT]^C AspI 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 315 GGACC G^G[AT]CC BbeI 1237 GGCGCC GGCGC^C BbsI 622 GTCTTC GAAGAC(2/6) BfaI 139 CTAG C^TAG BglII 44 AGATCT A^GATCT BlpI 1098 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1098 GCTAAGC GC^T[ACGT]AGC BpuAI 622 GTCTTC GAAGAC(2/6) BsaHI 1237 GGCGCC G[GA]^CG[CT]C BsiHKAI 591 GAGCTC G[AT]GC[AT]^C BspMI 965 ACCTGC ACCTGC(4/8) BsrBI 559 CCGCTC CCGCTC(-3/-3) BsrFI 613 GCCGGC [GA]^CCGG[CT] BstAPI 912 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 714 GGTCACC G^GT[ACGT]ACC BstPI 714 GGTCACC G^GT[ACGT]ACC BstXI 1154 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 44 AGATCT [GA]^GATC[CT] Bsu36I 68 CCTCAGG CC^T[ACGT]AGG CelII 1098 GCTAAGC GC^T[ACGT]AGC Cfr10I 613 GCCGGC [GA]^CCGG[CT] DsaI 970 CCGCGG C^C[GA][CT]GG Eco81I 68 CCTCAGG CC^T[ACGT]AGG EcoO65I 714 GGTCACC G^GT[ACGT]ACC EcoRI 350 GAATTC G^AATTC FauI 972 GCGGG CCCGC(4/6) Hin1I 1237 GGCGCC G[GA]^CG[CT]C HinfI 1050 GATTC G^A[ACGT]TC KasI 1237 GGCGCC G^GCGCC KspI 970 CCGCGG CCGC^GG MaeI 139 CTAG C^TAG MaeII 1013 ACGT A^CGT MflI 44 AGATCT [GA]^GATC[CT] MslI 231 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 613 GCCGGC GCC^GGC NarI 1237 GGCGCC GG^CGCC NgoMIV 613 GCCGGC G^CCGGC NlaIII 1161 CATG CATG^ PaeR7I 275 CTCGAG C^TCGAG PflFI 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 314 AGGACCT [GA]G^G[AT]CC[CT] SacI 591 GAGCTC GAGCT^C SacII 970 CCGCGG CCGC^GG SexAI 428 ACCAGGT A^CC[AT]GGT SfaNI 1140 GATGC GCATC(5/9) SfoI 1237 GGCGCC GGC^GCC TfiI 1050 GATTC G^A[AT]TC Tth111I 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 695 CCAGGGAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 275 CTCGAG C^TCGAG XhoII 44 AGATCT [GA]^GATC[CT] XmnI 546 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AhdI 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 943 GACAAGCTGTC AspEI 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 943 GACAAGCTGTC BalI 128 TGGCCA TGG^CCA 1151 TGGCCA BanI 229 GGCACC G^G[CT][GA]CC 1237 GGCGCC BpmI 391 CTGGAG CTGGAG(16/14) 541 CTGGAG BsaI 598 GAGACC GGTCTC(1/5) 732 GAGACC BsmAI 598 GAGAC GTCTC(1/5) 732 GAGAC BsmFI 341 GGGAC GGGAC(10/14) 679 GTCCC BsrDI 751 CATTGC GCAATG(2/0) 1127 GCAATG DpnII 45 GATC ^GATC 261 GATC DraII 314 AGGACCT [GA]G^G[ACGT]CC[CT] 889 GGGGCCC Eam1105I 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 943 GACAAGCTGTC EcoO109I 314 AGGACCT [GA]G^G[ACGT]CC[CT] 889 GGGGCCC HgaI 150 GACGC GACGC(5/10) 250 GACGC MboI 45 GATC ^GATC 261 GATC MluNI 128 TGGCCA TGG^CCA 1151 TGGCCA MscI 128 TGGCCA TGG^CCA 1151 TGGCCA MseI 820 TTAA T^TAA 904 TTAA NdeII 45 GATC ^GATC 261 GATC PstI 94 CTGCAG CTGCA^G 919 CTGCAG PvuII 502 CAGCTG CAG^CTG 1007 CAGCTG Sau3AI 45 GATC ^GATC 261 GATC SfcI 94 CTGCAG C^T[GA][CT]AG 919 CTGCAG SmaI 787 CCCGGG CCC^GGG 893 CCCGGG SmlI 275 CTCGAG C^T[CT][GA]AG 481 CTCAAG StuI 650 AGGCCT AGG^CCT 839 AGGCCT TaqI 276 TCGA T^CGA 308 TCGA Tru9I 820 TTAA T^TAA 904 TTAA Tsp509I 351 AATT ^AATT 674 AATT TthHB8I 276 TCGA T^CGA 308 TCGA XmaCI 787 CCCGGG C^CCGGG 893 CCCGGG XmaI 787 CCCGGG C^CCGGG 893 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AfaI AluI AvaI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI SapI Sau96I ScrFI StyI TseI Tsp45I TspRI