(Circular) (Six-base) MAPSORT of: pFN21AE4407.seq Check: 9463 from: 1 to: 5968 pFN21AE4407 5968 bp With 182 enzymes: SgfI * March 28, 2012 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2546 2555 4031 5415 5683 1632 Size: 467 447 9 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 467 447 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 2681 2953 4697 2681 Size: 272 1744 3952 Fragments arranged by size: 3952 1744 272 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3230 1184 Size: 2046 3922 AlwNI CAG_nnn'CTG Cuts at: 2923 3711 5283 2923 Size: 788 1572 3608 Fragments arranged by size: 3608 1572 788 ApaI G_GGCC'C Cuts at: 2899 2899 Size: 5968 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2355 3277 3503 3655 5764 1493 Size: 862 922 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 862 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2280 2792 2898 3293 1870 Size: 73 337 512 106 395 4545 Fragments arranged by size: 4545 512 395 337 106 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 3242 3289 4744 618 Size: 325 217 627 447 1008 47 1455 1842 Fragments arranged by size: 1842 1455 1008 627 447 325 217 47 BanII G_rGCy'C Cuts at: 1391 2454 2600 2700 2899 3287 1391 Size: 1063 146 100 199 388 4072 Fragments arranged by size: 4072 1063 388 199 146 100 BbeI G_GCGC'C Cuts at: 3246 3246 Size: 5968 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2620 928 Size: 971 721 4276 Fragments arranged by size: 4276 971 721 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 5070 3860 Size: 1210 4758 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2049 5963 2049 Size: 3914 2054 BlpI GC'TnA_GC Cuts at: 3104 3385 3104 Size: 281 5687 Bme1580I G_kGCm'C Cuts at: 1790 2482 2899 3219 4029 5185 1790 Size: 692 417 320 810 1156 2573 Fragments arranged by size: 2573 1156 810 692 417 320 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2901 3699 4657 Size: 416 53 83 399 24 1484 798 958 Cuts at: 4657 442 Size: 1753 Fragments arranged by size: 1753 1484 958 798 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2567 4628 1239 Size: 1178 150 2061 2579 Fragments arranged by size: 2579 2061 1178 150 Bpu10I CC'TnA_GC Cuts at: 2094 2094 Size: 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 4093 4958 5256 2471 Size: 1622 865 298 3183 Fragments arranged by size: 3183 1622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 2731 4631 882 Size: 617 1098 134 1900 2219 Fragments arranged by size: 2219 1900 1098 617 134 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3243 3775 4157 4792 Size: 53 83 186 936 1710 532 382 635 Cuts at: 4792 275 Size: 1451 Fragments arranged by size: 1710 1451 936 635 532 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4401 4799 5073 5220 5631 4401 Size: 398 274 147 411 4738 Fragments arranged by size: 4738 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2298 2312 2816 3104 1186 Size: 1112 14 504 288 4050 Fragments arranged by size: 4050 1112 504 288 14 BseYI C'CCAG_C Cuts at: 2481 2641 5171 5667 2481 Size: 160 2530 496 2782 Fragments arranged by size: 2782 2530 496 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3030 3050 3241 1163 Size: 573 1294 20 191 3890 Fragments arranged by size: 3890 1294 573 191 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2600 4029 4114 5185 2600 Size: 1429 85 1071 3383 Fragments arranged by size: 3383 1429 1071 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2454 2482 2600 2700 2899 3219 3287 Size: 399 664 28 118 100 199 320 68 Cuts at: 3287 4029 4114 5185 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 320 199 118 100 85 68 28 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2979 3323 844 Size: 2135 344 3489 Fragments arranged by size: 3489 2135 344 BsrBI CCG'CTC Cuts at: 1394 1823 2566 3855 1394 Size: 429 743 1289 3507 Fragments arranged by size: 3507 1289 743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2755 3139 4463 4637 65 Size: 1183 1507 384 1324 174 1396 Fragments arranged by size: 1507 1396 1324 1183 384 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2618 4612 4799 1700 Size: 110 808 1994 187 2869 Fragments arranged by size: 2869 1994 808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 2923 794 Size: 2129 3839 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 2719 1184 Size: 1535 4433 BstXI CCAn_nnnn'nTGG Cuts at: 3166 3166 Size: 5968 BstYI r'GATC_y Cuts at: 1972 2049 3298 3665 4050 4067 5519 5605 5617 Size: 77 1249 367 385 17 1452 86 12 Cuts at: 5617 5963 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1249 385 367 346 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 2074 2074 Size: 5968 BtgI C'CryG_G Cuts at: 513 1065 2975 513 Size: 552 1910 3506 Fragments arranged by size: 3506 1910 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 3484 4278 4298 3484 Size: 794 20 5154 Fragments arranged by size: 5154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2415 2620 Size: 54 1425 50 275 18 303 282 205 Cuts at: 2620 2933 3156 3415 4304 5933 8 Size: 313 223 259 889 1629 43 Fragments arranged by size: 1629 1425 889 313 303 282 275 259 223 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2588 2631 3898 1353 Size: 812 423 43 1267 3423 Fragments arranged by size: 3423 1267 812 423 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2598 2598 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2417 2546 2555 2567 4031 4628 Size: 393 467 318 129 9 12 1464 597 Cuts at: 4628 5415 5683 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1464 787 597 467 393 318 268 129 12 9 EcoO109I rG'GnC_Cy Cuts at: 2320 2895 2320 Size: 575 5393 EcoRI G'AATT_C Cuts at: 2355 3277 3655 2355 Size: 922 378 4668 Fragments arranged by size: 4668 922 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 2176 2731 2071 Size: 105 555 5308 Fragments arranged by size: 5308 555 105 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 2280 3142 3246 5115 1678 Size: 602 862 104 1869 2531 Fragments arranged by size: 2531 1869 862 602 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 2939 2971 4609 Size: 32 135 32 1095 32 240 32 1638 Cuts at: 4609 4641 4683 4715 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1638 1095 240 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3558 669 Size: 988 281 1374 246 3079 Fragments arranged by size: 3079 1374 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KasI G'GCGC_C Cuts at: 3242 3242 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5082 5266 1305 Size: 3777 184 2007 Fragments arranged by size: 3777 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 3158 10 Size: 54 1425 343 303 1023 2820 Fragments arranged by size: 2820 1425 1023 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 3926 4285 4444 518 Size: 648 1074 1686 359 159 2042 Fragments arranged by size: 2042 1686 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2509 2977 3014 3384 4061 5209 5454 1606 Size: 903 468 37 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 903 677 468 370 245 37 NaeI GCC'GGC Cuts at: 2620 2620 Size: 5968 NarI GG'CG_CC Cuts at: 3243 3243 Size: 5968 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5416 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NgoMIV G'CCGG_C Cuts at: 2618 2618 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 3326 4871 1132 Size: 97 2097 1545 2229 Fragments arranged by size: 2229 2097 1545 97 PasI CC'CwG_GG Cuts at: 1348 2687 2700 1348 Size: 1339 13 4616 Fragments arranged by size: 4616 1339 13 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5968 PfoI T'CCnGG_A Cuts at: 2631 2631 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2320 Size: 5968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 2895 2895 Size: 5968 PspXI vC'TCGA_Gb Cuts at: 2280 2280 Size: 5968 PstI C_TGCA'G Cuts at: 830 2103 2928 3320 830 Size: 1273 825 392 3478 Fragments arranged by size: 3478 1273 825 392 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2509 3014 2509 Size: 505 5463 SacI G_AGCT'C Cuts at: 2600 2600 Size: 5968 SacII CC_GC'GG Cuts at: 2978 2978 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2165 2588 2631 2165 Size: 423 43 5502 Fragments arranged by size: 5502 423 43 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SexAI A'CCwGG_T Cuts at: 2433 2433 Size: 5968 SfcI C'TryA_G Cuts at: 826 1045 2099 2924 3316 4451 5132 5323 826 Size: 219 1054 825 392 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 825 681 392 219 191 SfoI GGC'GCC Cuts at: 3244 3244 Size: 5968 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 2794 2900 3295 1872 Size: 922 106 395 4545 Fragments arranged by size: 4545 922 395 106 SmlI C'TyrA_G Cuts at: 820 1017 1943 2280 2486 4072 4973 5235 820 Size: 197 926 337 206 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 337 262 206 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 2657 2846 1517 Size: 1140 189 4639 Fragments arranged by size: 4639 1140 189 StyI C'CwwG_G Cuts at: 513 1065 2082 2841 3126 3407 513 Size: 552 1017 759 285 281 3074 Fragments arranged by size: 3074 1017 759 552 285 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2896 2974 3995 4012 4165 4350 4795 Size: 1255 1316 78 1021 17 153 185 445 Cuts at: 4795 325 Size: 1498 Fragments arranged by size: 1498 1316 1255 1021 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4214 5926 Size: 274 80 33 51 1443 324 1913 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1913 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5928 5960 5928 Size: 32 5936 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 4567 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 2707 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 2280 1943 Size: 337 5631 XmaI C'CCGG_G Cuts at: 1870 2792 2898 3293 1870 Size: 922 106 395 4545 Fragments arranged by size: 4545 922 395 106 XmnI GAAnn'nnTTC Cuts at: 2555 4097 2555 Size: 1542 4426 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SfiI SrfI SwaI