(Linear) (Six-base) MAPSORT of: KIEE4407.seq Check: 448 from: 1 to: 1263 KIEE4407.orf With 182 enzymes: SgfI * March 22, 2012 17:56 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 541 550 1263 Size: 94 447 9 713 Fragments arranged by size: 713 447 94 9 AhdI GACnn_n'nnGTC Cuts at: 0 676 948 1263 Size: 676 272 315 Fragments arranged by size: 676 315 272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1225 1263 Size: 1225 38 AlwNI CAG_nnn'CTG Cuts at: 0 918 1263 Size: 918 345 ApaI G_GGCC'C Cuts at: 0 894 1263 Size: 894 369 ApoI r'AATT_y Cuts at: 0 350 1263 Size: 350 913 AvaI C'yCGr_G Cuts at: 0 275 787 893 1263 Size: 275 512 106 370 Fragments arranged by size: 512 370 275 106 BanI G'GyrC_C Cuts at: 0 229 1237 1263 Size: 229 1008 26 Fragments arranged by size: 1008 229 26 BanII G_rGCy'C Cuts at: 0 449 595 695 894 1263 Size: 449 146 100 199 369 Fragments arranged by size: 449 369 199 146 100 BbeI G_GCGC'C Cuts at: 0 1241 1263 Size: 1241 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 615 1263 Size: 615 648 BglII A'GATC_T Cuts at: 0 44 1263 Size: 44 1219 BlpI GC'TnA_GC Cuts at: 0 1099 1263 Size: 1099 164 Bme1580I G_kGCm'C Cuts at: 0 477 894 1214 1263 Size: 477 417 320 49 Fragments arranged by size: 477 417 320 49 BmrI ACTGGGnnnn_n' Cuts at: 0 896 1263 Size: 896 367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 562 1263 Size: 412 150 701 Fragments arranged by size: 701 412 150 Bpu10I CC'TnA_GC Cuts at: 0 89 1263 Size: 89 1174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 1263 Size: 466 797 BsaI GGTCTCn'nnnn_ Cuts at: 0 592 726 1263 Size: 592 134 537 Fragments arranged by size: 592 537 134 BsaHI Gr'CG_yC Cuts at: 0 1238 1263 Size: 1238 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 293 307 811 1099 1263 Size: 293 14 504 288 164 Fragments arranged by size: 504 293 288 164 14 BseYI C'CCAG_C Cuts at: 0 476 636 1263 Size: 476 160 627 Fragments arranged by size: 627 476 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1025 1045 1236 1263 Size: 1025 20 191 27 Fragments arranged by size: 1025 191 27 20 BsiHKAI G_wGCw'C Cuts at: 0 595 1263 Size: 595 668 Bsp1286I G_dGCh'C Cuts at: 0 449 477 595 695 894 1214 1263 Size: 449 28 118 100 199 320 49 Fragments arranged by size: 449 320 199 118 100 49 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 974 1263 Size: 974 289 BsrBI CCG'CTC Cuts at: 0 561 1263 Size: 561 702 BsrDI GCAATG_nn' Cuts at: 0 750 1134 1263 Size: 750 384 129 Fragments arranged by size: 750 384 129 BsrFI r'CCGG_y Cuts at: 0 613 1263 Size: 613 650 BstAPI GCAn_nnn'nTGC Cuts at: 0 918 1263 Size: 918 345 BstEII G'GTnAC_C Cuts at: 0 714 1263 Size: 714 549 BstXI CCAn_nnnn'nTGG Cuts at: 0 1161 1263 Size: 1161 102 BstYI r'GATC_y Cuts at: 0 44 1263 Size: 44 1219 Bsu36I CC'TnA_GG Cuts at: 0 69 1263 Size: 69 1194 BtgI C'CryG_G Cuts at: 0 970 1263 Size: 970 293 EaeI y'GGCC_r Cuts at: 0 128 410 615 928 1151 1263 Size: 128 282 205 313 223 112 Fragments arranged by size: 313 282 223 205 128 112 EarI CTCTTCn'nnn_ Cuts at: 0 160 583 626 1263 Size: 160 423 43 637 Fragments arranged by size: 637 423 160 43 EcoICRI GAG'CTC Cuts at: 0 593 1263 Size: 593 670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 412 541 550 562 1263 Size: 94 318 129 9 12 701 Fragments arranged by size: 701 318 129 94 12 9 EcoO109I rG'GnC_Cy Cuts at: 0 315 890 1263 Size: 315 575 373 Fragments arranged by size: 575 373 315 EcoRI G'AATT_C Cuts at: 0 350 1263 Size: 350 913 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 66 171 726 1263 Size: 66 105 555 537 Fragments arranged by size: 555 537 105 66 HaeII r_GCGC'y Cuts at: 0 275 1137 1241 1263 Size: 275 862 104 22 Fragments arranged by size: 862 275 104 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 662 694 934 966 1263 Size: 662 32 240 32 297 Fragments arranged by size: 662 297 240 32 32 KasI G'GCGC_C Cuts at: 0 1237 1263 Size: 1237 26 MscI TGG'CCA Cuts at: 0 130 1153 1263 Size: 130 1023 110 Fragments arranged by size: 1023 130 110 MslI CAynn'nnrTG Cuts at: 0 235 1263 Size: 235 1028 MspA1I CmG'CkG Cuts at: 0 504 972 1009 1263 Size: 504 468 37 254 Fragments arranged by size: 504 468 254 37 NaeI GCC'GGC Cuts at: 0 615 1263 Size: 615 648 NarI GG'CG_CC Cuts at: 0 1238 1263 Size: 1238 25 NgoMIV G'CCGG_C Cuts at: 0 613 1263 Size: 613 650 PasI CC'CwG_GG Cuts at: 0 682 695 1263 Size: 682 13 568 Fragments arranged by size: 682 568 13 PfoI T'CCnGG_A Cuts at: 0 626 1263 Size: 626 637 PpuMI rG'GwC_Cy Cuts at: 0 315 1263 Size: 315 948 PspOMI G'GGCC_C Cuts at: 0 890 1263 Size: 890 373 PspXI vC'TCGA_Gb Cuts at: 0 275 1263 Size: 275 988 PstI C_TGCA'G Cuts at: 0 98 923 1263 Size: 98 825 340 Fragments arranged by size: 825 340 98 PvuII CAG'CTG Cuts at: 0 504 1009 1263 Size: 504 505 254 Fragments arranged by size: 505 504 254 SacI G_AGCT'C Cuts at: 0 595 1263 Size: 595 668 SacII CC_GC'GG Cuts at: 0 973 1263 Size: 973 290 SapI GCTCTTCn'nnn_ Cuts at: 0 160 583 626 1263 Size: 160 423 43 637 Fragments arranged by size: 637 423 160 43 SexAI A'CCwGG_T Cuts at: 0 428 1263 Size: 428 835 SfcI C'TryA_G Cuts at: 0 94 919 1263 Size: 94 825 344 Fragments arranged by size: 825 344 94 SfoI GGC'GCC Cuts at: 0 1239 1263 Size: 1239 24 SmaI CCC'GGG Cuts at: 0 789 895 1263 Size: 789 106 368 Fragments arranged by size: 789 368 106 SmlI C'TyrA_G Cuts at: 0 275 481 1263 Size: 275 206 782 Fragments arranged by size: 782 275 206 StuI AGG'CCT Cuts at: 0 652 841 1263 Size: 652 189 422 Fragments arranged by size: 652 422 189 StyI C'CwwG_G Cuts at: 0 77 836 1121 1263 Size: 77 759 285 142 Fragments arranged by size: 759 285 142 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 891 969 1263 Size: 891 78 294 Fragments arranged by size: 891 294 78 TatI w'GTAC_w Cuts at: 0 296 1263 Size: 296 967 Tth111I GACn'n_nGTC Cuts at: 0 712 1263 Size: 712 551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 702 1263 Size: 702 561 XhoI C'TCGA_G Cuts at: 0 275 1263 Size: 275 988 XmaI C'CCGG_G Cuts at: 0 787 893 1263 Size: 787 106 370 Fragments arranged by size: 787 370 106 XmnI GAAnn'nnTTC Cuts at: 0 550 1263 Size: 550 713 Enzymes that do cut: AcuI AhdI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI ZraI