(Circular) (Six-base) MAPSORT of: pFN21AE3913.seq Check: 2388 from: 1 to: 5335 pFN21AE3913 5335 bp With 182 enzymes: SgfI * March 28, 2012 11:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2099 3145 278 Size: 53 83 186 936 563 1046 2468 Fragments arranged by size: 2468 1046 936 563 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 1937 Size: 741 4594 Acc65I G'GTAC_C Cuts at: 2337 2368 2656 2337 Size: 31 288 5016 Fragments arranged by size: 5016 288 31 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4782 5050 1632 Size: 1766 1384 268 1917 Fragments arranged by size: 1917 1766 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 4234 5211 1203 Size: 3031 977 1327 Fragments arranged by size: 3031 1327 977 AgeI A'CCGG_T Cuts at: 2331 4166 2331 Size: 1835 3500 AhdI GACnn_n'nnGTC Cuts at: 4064 4064 Size: 5335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2213 1184 Size: 1029 4306 AlwNI CAG_nnn'CTG Cuts at: 3078 4650 3078 Size: 1572 3763 ApaLI G'TGCA_C Cuts at: 2198 3392 4548 2198 Size: 1194 1156 2985 Fragments arranged by size: 2985 1194 1156 ApoI r'AATT_y Cuts at: 1493 2644 2870 3022 5131 1493 Size: 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 226 152 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2132 2510 2660 1870 Size: 73 189 378 150 4545 Fragments arranged by size: 4545 378 189 150 73 BamHI G'GATC_C Cuts at: 2665 3032 2665 Size: 367 4968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2337 2368 2656 4111 Size: 325 217 627 248 302 31 288 1455 Cuts at: 4111 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 302 288 248 217 31 BanII G_rGCy'C Cuts at: 1391 2654 1391 Size: 1263 4072 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 5335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2099 2104 2542 928 Size: 971 200 5 438 3721 Fragments arranged by size: 3721 971 438 200 5 BbvCI CC'TCA_GC Cuts at: 2576 2576 Size: 5335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3526 3560 1903 Size: 34 1589 34 3678 Fragments arranged by size: 3678 1589 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 1548 Size: 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2123 2570 Size: 107 122 71 631 605 337 114 447 Cuts at: 2570 3946 136 Size: 1376 1525 Fragments arranged by size: 1525 1376 631 605 447 337 122 114 107 71 BglII A'GATC_T Cuts at: 5330 5330 Size: 5335 BlpI GC'TnA_GC Cuts at: 2752 2752 Size: 5335 Bme1580I G_kGCm'C Cuts at: 1790 2202 3396 4552 1790 Size: 412 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3066 4024 442 Size: 416 53 83 399 24 1649 958 1753 Fragments arranged by size: 1753 1649 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3995 1239 Size: 2756 2579 Bpu10I CC'TnA_GC Cuts at: 2576 2576 Size: 5335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3460 4325 4623 3460 Size: 865 298 4172 Fragments arranged by size: 4172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 882 Size: 617 2499 2219 Fragments arranged by size: 2499 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2096 2268 3142 Size: 53 83 186 936 503 60 172 874 Cuts at: 3142 3524 4159 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 874 635 503 382 186 172 83 60 53 BsaWI w'CCGG_w Cuts at: 2331 3768 4166 4440 4587 4998 2331 Size: 1437 398 274 147 411 2668 Fragments arranged by size: 2668 1437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5110 Fragments arranged by size: 5110 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 1186 Size: 859 4476 BseYI C'CCAG_C Cuts at: 4538 5034 4538 Size: 496 4839 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4762 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 2785 3546 4574 1540 Size: 275 186 589 195 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 275 195 186 BsiHKAI G_wGCw'C Cuts at: 2202 2519 3396 3481 4552 2202 Size: 317 877 85 1071 2985 Fragments arranged by size: 2985 1071 877 317 85 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2519 2654 3396 3481 4552 1391 Size: 399 412 317 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 317 135 85 BspEI T'CCGG_A Cuts at: 4998 4998 Size: 5335 BspHI T'CATG_A Cuts at: 3224 4954 3224 Size: 1730 3605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 2409 2690 844 Size: 1340 225 281 3489 Fragments arranged by size: 3489 1340 281 225 BsrBI CCG'CTC Cuts at: 1394 1823 2161 3222 1394 Size: 429 338 1061 3507 Fragments arranged by size: 3507 1061 429 338 BsrDI GCAATG_nn' Cuts at: 65 1248 3830 4004 65 Size: 1183 2582 174 1396 Fragments arranged by size: 2582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2331 3979 4166 1700 Size: 110 521 1648 187 2869 Fragments arranged by size: 2869 1648 521 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5335 BssSI C'ACGA_G Cuts at: 3395 4407 3395 Size: 1012 4323 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5335 BstBI TT'CG_AA Cuts at: 4139 4139 Size: 5335 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 3890 BstYI r'GATC_y Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330 Size: 693 367 385 17 1452 86 12 346 Cuts at: 5330 1972 Size: 1977 Fragments arranged by size: 1977 1452 693 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2148 2490 2584 513 Size: 552 957 126 342 94 3264 Fragments arranged by size: 3264 957 552 342 126 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4596 Fragments arranged by size: 4596 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3645 3665 2851 Size: 794 20 4521 Fragments arranged by size: 4521 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2137 2172 529 Size: 35 1573 35 3692 Fragments arranged by size: 3692 1573 35 35 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2273 4342 809 Size: 507 957 2069 1802 Fragments arranged by size: 2069 1802 957 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2782 3671 5300 Size: 54 1425 50 275 18 952 889 1629 Cuts at: 5300 8 Size: 43 Fragments arranged by size: 1629 1425 952 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2782 1537 Size: 275 970 4090 Fragments arranged by size: 4090 970 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3265 1353 Size: 1912 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2620 3922 4296 4442 387 Size: 1064 138 1031 1302 374 146 1280 Fragments arranged by size: 1302 1280 1064 1031 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3398 3995 4782 5050 1239 Size: 393 1766 597 787 268 1524 Fragments arranged by size: 1766 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2464 2405 Size: 59 5276 EcoO109I rG'GnC_Cy Cuts at: 2124 2124 Size: 5335 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FspI TGC'GCA Cuts at: 2405 3841 2405 Size: 1436 3899 HaeII r_GCGC'y Cuts at: 1678 2039 4482 1678 Size: 361 2443 2531 Fragments arranged by size: 2531 2443 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373 Size: 32 135 32 2404 32 42 32 2626 Fragments arranged by size: 2626 2404 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 2679 2925 669 Size: 988 281 235 506 246 3079 Fragments arranged by size: 3079 988 506 281 246 235 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KasI G'GCGC_C Cuts at: 2035 2035 Size: 5335 KpnI G_GTAC'C Cuts at: 2341 2372 2660 2341 Size: 31 288 5016 Fragments arranged by size: 5016 288 31 MfeI C'AATT_G Cuts at: 2934 2934 Size: 5335 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4449 4633 1305 Size: 3144 184 2007 Fragments arranged by size: 3144 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 3293 3652 3811 518 Size: 648 1215 912 359 159 2042 Fragments arranged by size: 2042 1215 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2586 2751 3428 4576 4821 1606 Size: 418 562 165 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 562 418 245 165 NarI GG'CG_CC Cuts at: 2036 2036 Size: 5335 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4783 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2782 2782 Size: 5335 NspI r_CATG'y Cuts at: 1132 1229 2693 4238 1132 Size: 97 1464 1545 2229 Fragments arranged by size: 2229 1545 1464 97 PasI CC'CwG_GG Cuts at: 1348 2614 1348 Size: 1266 4069 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 3964 PfoI T'CCnGG_A Cuts at: 2553 2553 Size: 5335 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4917 4949 3404 Size: 32 1481 32 3790 Fragments arranged by size: 3790 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PspXI vC'TCGA_Gb Cuts at: 2510 2510 Size: 5335 PstI C_TGCA'G Cuts at: 830 2687 830 Size: 1857 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 RsrII CG'GwC_CG Cuts at: 2052 2052 Size: 5335 SacII CC_GC'GG Cuts at: 2025 2587 2025 Size: 562 4773 SalI G'TCGA_C Cuts at: 1936 2677 1936 Size: 741 4594 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 3583 5295 3583 Size: 1712 3623 SfcI C'TryA_G Cuts at: 826 1045 2683 3818 4499 4690 826 Size: 219 1638 1135 681 191 1471 Fragments arranged by size: 1638 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2037 2037 Size: 5335 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2662 1872 Size: 790 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 3439 4340 4602 820 Size: 197 926 567 929 901 262 1553 Fragments arranged by size: 1553 929 926 901 567 262 197 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2693 1132 Size: 1561 3774 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2774 513 Size: 552 1709 3074 Fragments arranged by size: 3074 1709 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3362 3379 3532 3717 4162 325 Size: 1255 1782 17 153 185 445 1498 Fragments arranged by size: 1782 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3581 5293 96 Size: 274 80 33 51 1443 1604 1712 138 Fragments arranged by size: 1712 1604 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5295 5327 5295 Size: 32 5303 Tth111I GACn'n_nGTC Cuts at: 1316 2537 1316 Size: 1221 4114 XbaI T'CTAG_A Cuts at: 2671 2671 Size: 5335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2380 Size: 5335 XhoI C'TCGA_G Cuts at: 1943 2510 1943 Size: 567 4768 XmaI C'CCGG_G Cuts at: 1870 2660 1870 Size: 790 4545 XmnI GAAnn'nnTTC Cuts at: 2354 3464 2354 Size: 1110 4225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2097 3143 276 Size: 53 83 186 936 563 1046 2468 Fragments arranged by size: 2468 1046 936 563 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII SacI SanDI SapI SexAI SfiI SrfI SwaI